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Umfang: 1 online resource (432 p.)

ISBN: 9786611055523 , 9781281055521 , 1281055522 , 9780080554884 , 0080554881

Serie: Methods in enzymology ; v. 432

Inhalt: This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species within Cells; Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry; Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry; Structure-specific, quantitative methods for ""lipidomic"" ana

Anmerkung: Description based upon print version of record. , Front Cover; Lipidomics and Bioactive Lipids: Mass-Spectrometry-Based Lipid Analysis; Copyright Page; Contents; Contributors; Preface; Volumes in Series; Chapter 1: Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species Within Cells; 1. Introduction; 2. Reagents; 2.1. Cell culture; 2.2. Standards; 2.3. Extraction and purification; 3. Methods; 3.1. Cell culture; 4. Results; 4.1. Qualitative analysis; 4.2. Quantitative analysis; 5. Conclusions; Acknowledgments; References , Chapter 2: Glycerophospholipid Identification and Quantitation by Electrospray Ionization Mass Spectrometry1. Introduction; 2. Nomenclature; 3. Mass Spectrometry; 4. General Strategy for Phospholipid Isolation and Mass Spectral Analysis; 5. Extraction and Mass Spectral Analysis of Global Glycerophospholipids; 5.1. Phospholipid extraction from cultured cells; 5.2. Phospholipid extraction from tissue; 5.3. Direct infusion mass spectrometry of phospholipid extracts; 5.4. LC-MS analysis (quantitation) of phospholipid extracts; 6. Polyphosphoinositide Extraction and Mass Spectral Analysis , 6.1. Extraction of polyphosphoinositides from cultured cells6.2. Extraction of polyphosphoinositides from tissue; 6.3. Direct-infusion mass spectral analysis of polyphosphoinositides; 6.4. Deacylation of GPInsPn lipids; 6.5. LC-MS analysis of deacylated GPInsPn compounds; 7. Computational Analysis of Mass Spectral Data; 7.1. Direct infusion (intra-source separation); 7.2. LC-MS data analysis; Acknowledgments; References; Chapter 3: Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry; 1. Introduction; 2. Methods , 2.1. Sample collection2.2. Eicosanoid isolation; 2.3. Reverse-phase liquid chromatography; 2.4. Chiral chromatography; 2.5. Mass spectrometry; 2.6. Quantitation; 3. Results and Discussion; 3.1. MRM transition selection; 3.2. Stereoisomer detection; 3.3. Lower limit of detection; 3.4. Recoveries; 3.5. Miscellany; 3.6. Summary; Acknowledgments; References; Chapter 4: Structure-Specific, Quantitative Methods for Analysis of Sphingolipids by Liquid Chromatography-Tandem Mass Spectrometry: ''Inside-Out'' Sphingolipidomics; 1. Introduction: An Overview of Sphingolipid Structures and Nomenclature , 2. Analysis of Sphingolipids by Mass Spectrometry2.1. Electron ionization-mass spectrometry; 2.2. Fast atom bombardment and liquid secondary ionization mass spectrometry; 2.3. Electrospray ionization; 2.4. Liquid chromatography, electrospray ionization, mass spectrometry and tandem mass spectrometry; 2.5. MALDI, mass spectrometry, and tandem mass spectrometry; 3. Analysis of Sphingolipids by ''OMIC'' Approaches; 4. Materials and Methods; 5. Materials; 5.1. Biological samples; 6. Extraction; 6.1. Preparation of samples for LC-MS/MS , 7. Identification of the Molecular Species by Tandem Mass Spectrometry , English

Weitere Ausg.: ISBN 9780123738950

Weitere Ausg.: ISBN 0123738954

Sprache: Englisch

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Umfang: Online-Ressource (387 S.)

Ausgabe: Online-Ausg. 2009 Electronic reproduction; Available via World Wide Web

ISBN: 9780123738950

Serie: Methods in enzymology; 432

Inhalt: This volume in the well-established Methods in Enzymologyseries features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species within Cells, Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry, Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry, Structure-specific, quantitative methods for lipidomic analysis of sphingolipids by tandem mass spectrometry, Analysis of Ubiquinones, Dolichols and Dolichol Diphosphate-Oligosaccharides by Liquid Chromatography Electrospray Ionization Mass Spectrometry, Extraction and Analysis of Sterols in Biological Matrices by High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry, The Lipid Maps Initiative in Lipidomics, Basic analytical systems for lipidomics by mass spectrometry in Japan, The European Lipidomics Initiative Enabling technologies, Lipidomic analysis of Signalling Pathways, Bioinformatics for Lipidomics, Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins and Protectins, A guide to biochemical systems modeling of sphingolipids for the biochemist, and Quantitation and Standardization of Lipid Internal Standards for Mass Spectroscopy.

Anmerkung: Includes bibliographical references and indexes , Electronic reproduction; Available via World Wide Web

Weitere Ausg.: Erscheint auch als Druck-Ausgabe Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis Amsterdam [u.a.] : Elsevier, Acad. Press, 2007 ISBN 9780123738950

Sprache: Englisch

Fachgebiete: Biologie

Schlagwort(e): Lipidstoffwechsel ; Massenspektrometrie ; Labortechnik

URL: Volltext

URL: Inhaltsverzeichnis

UID:

Umfang: 1 Online-Ressource

ISBN: 9780123738950 , 0123738954

Serie: Methods in enzymology 432

Sprache: Englisch

Fachgebiete: Biologie

Schlagwort(e): Lipidstoffwechsel ; Massenspektrometrie ; Labortechnik

URL: Volltext