UID:
almafu_9958130424702883
Format:
1 online resource (673 p.)
ISBN:
9786611189068
,
9781281189066
,
1281189065
,
9780080560441
,
008056044X
Series Statement:
Methods in enzymology ; v. 436
Content:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect - full-text online of volume
Note:
Description based upon print version of record.
,
Front Cover; Globins and Other Nitric Oxide-Reactive Proteins; Copyright Page; Contents; Contributors; Volumes in Series; Section I: Nitric Oxide: Chemical and Analytical Methods; Chapter 1: Chemistry of Nitric Oxide and Related Species; 1. Introduction; 2. Chemistry of NO; 2.1. Properties of the NO molecule; 2.2. Reaction of NO with oxygen; 3. Compounds Related to NO; 3.1. Nitroxyl HNO; 3.2. Reactions of HNO; 4. Nitrous Acid, Nitrosation, and S-Nitrosothiols; 7. Nitrosative Stress and Reactive Nitrogen Species; 10. Scavenging NO with Metal Complexes
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Chapter 2: Delivery of Nitric Oxide for Analysis of the Function of Cytochrome c'Chapter 3: The Preparation and Purification of NO Gas and the Use of NO Releasers: The Application of NO Donors and Other Agents of Nitrosative Stres...; 1. Introduction; 2. Supply of NO; 2.1. NO from cylinders and lecture bottles; 2.2. Generation of NO in the laboratory; 2.3. Determination of NO concentration; 3. NO Releasers; 3.1. S-nitrosothiols; 3.2. Metal nitrosyl complexes; 3.3. NOR, NOC, and NONOate compounds; 4.1. Cellular fate of NO2 and the level of nitrosative stress
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4.2. Microbes and nitrosative stressChapter 4: The Chemistry of Peroxynitrite: Implications for Biological Activity; 1. Introduction; 2. Mechanism of Peroxynitrite Decomposition in Aqueous Solutions; 4. The Radical Model In Vivo; 5. Nitration of Tyrosine; 6. Summary; Chapter 5: Nitric Oxide Selective Electrodes; 1. Significance of NO in Life Science; 3. Advantages of Electrochemical Sensors for Determination of NO; 5.1. Clark-type NO electrodes; 5.2. Modified carbon-fiber NO microelectrodes; 5.3. Integrated NO microelectrodes; 5.4. Other NO electrodes
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6.1. Calibration using an NO standard solution6.2. Calibration based on decomposition of SNAP; 6.3. Calibration based on chemical generation of NO; 7.1. Sensitivity and detection limit; 7.2. Selectivity; 7.3. Response time; 7.4. Effect of temperature and pH on NO electrodes; 8. Selected Applications of NO Electrodes; 9. Concluding Remarks and Other Directions; Acknowledgments; References; Chapter 6: NO, N2O, and O2 Reaction Kinetics: Scope and Limitations of the Clark Electrode; 1. Introduction; 2. Materials; 5.1. Determination of NO reduction activity of Nor
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5.2. Determination of N2O reduction activity of N2OR5.3. O2 measurements; Chapter 7: Chemiluminescence Quantification of NO and Its Derivatives in Liquid Samples; 1. Introduction; 1.1. The myriad roles of NO; 1.2. The chemistry of NO; 1.3. The NO-ozone reaction and the chemiluminescence analyzer; 2. Methods; 2.1. Determination of NO-2 concentration in liquid samples; 2.2. Determination of NO-2 , NO-3 , and S-nitrosothiol concentrations in liquid samples; 2.3. Determination of SNO concentration in liquid samples; 3. Applications; 3.1. Measurement of nitrate/nitrite in cell supernatants
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3.2. Measurement of SNO in murine macrophage cell lysates
Additional Edition:
ISBN 9780123742773
Additional Edition:
ISBN 0123742773
Language:
English
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