UID:
almafu_9958131692902883
Umfang:
1 online resource (610 p.)
ISBN:
9786611279462
,
9781281279460
,
1281279463
,
9780080570075
,
0080570070
Serie:
Methods in enzymology, v. 439
Inhalt:
The critically acclaimed laboratory standard for more than 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect - full-text online of volume 1
Anmerkung:
Description based upon print version of record.
,
Cover; Contents; Contributors; Methods in Enzymology; Chapter 1: Human Pancreatic Duct Epithelial Cell Model for KRAS Transformation; 1. Introduction; 2. Description of Methods; 3. Concluding Remarks; Acknowledgment; References; Chapter 2: Mouse Model for NRAS-Induced Leukemogenesis; 1. Introduction; 2. Modeling NRAS Leukemogenesis in Mice Using the BMT Method; 3. Characterization of Diseased NRAS Mice; Acknowledgment; References; Chapter 3: Inducible BRAF Suppression Models for Melanoma Tumorigenesis; 1. Introduction; 2. Materials and Methods; 3. Conclusion; Acknowledgment; References
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Chapter 4: A Method to Generate Genetically Defined Tumors in Pigs1. Introduction; 2. Overview; 3. Derivation of Cell Line from Swine Ear Notches; 4. Creation of Six Gene-Transformed Cells; 5. Injecting Cells into Pigs; 6. Concluding Remarks; References; Chapter 5: Tools to Study the Function of the Ras-Related, Estrogen-Regulated Growth Inhibitor in Breast Cancer; 1. Introduction; 2. Molecular Constructs; 3. Cell Culture and Expression Vector Transfection and Infection; 4. Cell Growth and Invasion Assays
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5. Generation and Validation of Rerg Antibodies for Western Blotting and Immunohistochemistry6. Transient and Stable Expression of Wild-Type and Mutant Rerg Proteins; 7. Establishment of a Tamoxifen-Inducible Rerg Protein Expression System; 8. Suppression of Rerg Expression by RNA Interference; 9. Summary; Acknowledgments; References; Chapter 6: K-Ras-Driven Pancreatic Cancer Mouse Model for Anticancer Inhibitor Analyses; 1. Introduction; 2. Pancreatic Ductal Adenocarcinoma Models; 3. Imaging; 4. Drug Delivery; 5. End Point Analysis; 6. Model Fidelity for Preclinical Testing
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7. Novel Therapeutics and Preclinical Trial Design8. Conclusions; References; Chapter 7: Analysis of K-Ras Phosphorylation, Translocation, and Induction of Apoptosis; 1. Introduction; 2. Analysis of K-Ras Phosphorylation by Metabolic Labeling with 32P; 3. Phoshorylation of Endogenous K-Ras in T Cells; 4. Analysis of K-Ras Farnesyl-Electrostatic Switch by Live Cell Imaging; 5. Targeting of PKC to Activated K-Ras with the Ras Binding Domain of Raf-1 (RBD); 6. Single-Cell Fluorescent Analysis of Apoptosis: YFP-Caspase Sensor; References
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Chapter 8: Regulation of RhoBTB2 by the Cul3 Ubiquitin Ligase Complex1. Introduction; 2. Methods; References; Chapter 9: Characterization of EHT 1864, a Novel Small Molecule Inhibitor of Rac Family Small GTPases; 1. Introduction; 2. Experimental Procedures; 3. Concluding Remarks; Acknowledgments; References; Chapter 10: Analysis of Rho-GTPase Mimicry by a Family of Bacterial Type III Effector Proteins; 1. Introduction; 2. Analysis of WxxxE Effectors as Rho-GTPase Mimics in Mammalian Cells; 3. Analysis of Small G-Protein Mimicry Using Yeast as a Model System; 4. Concluding Remarks
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Acknowledgments
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English
Weitere Ausg.:
ISBN 9780123743114
Weitere Ausg.:
ISBN 0123743117
Sprache:
Englisch
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