Umfang:
Online-Ressource (XLVIII, 493 S.)
Ausgabe:
Online-Ausg. 2009 Electronic reproduction; Available via World Wide Web
ISBN:
9780123743121
Serie:
Methods in enzymology 442
Inhalt:
The 2002 Nobel Prize in Physiology or Medicine was awarded to Sydney Brenner (United Kingdom), H. Robert Horvitz (US) and John E. Sulston (UK) for their discoveries concerning genetic regulation of organ development and programmed cell death. Cell death is a fundamental aspect of embryonic development, normal cellular turnover and maintenance of homeostasis (maintaining a stable, constant environment) on the one hand, and aging and disease on the other. This volume addresses the significant advances with the techniques that are being used to analyze cell death. * This volume provides the necessary, trusted methods to carry out this research on the latest techniques. Once researchers understand the molecular mechanisms of the apoptotic pathways, they can begin to develop new therapies. * Presents key methods on studying tumors and how these cancer cells evade cell death. * Eliminates searching through many different sources to avoid pitfalls so the same mistakes are not made over and over.
Anmerkung:
Includes bibliographical references and index
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Front Cover; Methods in Enzymology; Copyright Page; Contents; Contributors; Preface; Volumes in Series; Chapter 1: Analyzing Morphological and Ultrastructural Features in Cell Death; 1. Introduction; 2. The Contribution of Morphological Analyses; 3. Light Microscopy; 4. Sample Processing for Light Microscopy; 5. Electron Microscopy; 6. Scanning Electron Microscopy (SEM); References; Chapter 2: Evaluation of Some Cell Death Features by Real Time Real Space Microscopy; 1. Mitochondria and Cell Death; 2. Bax, Mitochondria, and Calcium Signaling
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3. Calcium Signaling and Neurodegenerative ProcessesAcknowledgments; References; Chapter 3: Analysis of Apoptotic Pathways by Multiparametric Flow Cytometry: Application to HIV Infection; 1. Introduction; 2. Intracellular Expression of Bcl-2 Family Members and Relationship with Apoptosis; 3. Analysis of Mitochondrial Transmembrane Potential by DiOC6(3) Staining; 4. Analysis of DNA Fragmentation by ISNT and TUNEL Assays; 5. Analysis of the Loss of Plasma Membrane Asymmetry with the Annexin-V Assay; 6. Analysis of the Loss of Plasma Membrane Integrity with the 7-Amino Actinomycin D Assay
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7. A Flow Cytometry Assay to Quantify Cell-Mediated Cytotoxicity8. Conclusions; Acknowledgments; References; Chapter 4: Methods to Analyze the Palmitoylated CD95 High Molecular Weight Death-Inducing Signaling Complex; 1. Introduction; 2. Use of Sucrose Density Gradients for Analysis of hiDISC; 3. Association of CD95hi with Active Caspase-8 (Reverse DISC-IP); 4. Role of Palmitoylation for CD95 Signaling; 5. Conclusions; Acknowledgments; References; Chapter 5: Immunomagnetic Isolation of Tumor Necrosis Factor Receptosomes; 1. Introduction; 2. Compartmentalization of TNF-R1 Apoptosis Signaling
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3. Methods for Magnetic Labeling and Isolation of Subcellular Signaling Structures4. Experimental Procedures; 5. Conclusions; Acknowledgments; References; Chapter 6: Analyzing Lipid Raft Dynamics during Cell Apoptosis; 1. Introduction; 2. The Contribution of Biochemical Approaches; 3. The Contribution of Morphological Analyses; References; Chapter 7: Apoptosome Assembly; 1. Introduction; 2. Apoptosome for Caspase-9 Activation; 3. Dark Apoptosome for Dronc Activation; 4. CED-4 Apoptosome for CED-3 Activation; 5. Conclusion; Acknowledgments; References
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Chapter 8: Caspases: Determination of Their Activities in Apoptotic Cells1. Introduction; 2. Methods for Analysis of Caspase Processing, Activation, and Substrate Cleavage; 3. Conclusion; Acknowledgments; References; Chapter 9: Lysosomes in Apoptosis; 1. Introduction; 2. Lysosomal Membrane Permeabilization Induction; 3. Methods Used for the Detection of Lysosome Integrity; 4. Staining for Acid Compartments/Vesicles; 5. Validation of Lysosomal Membrane Permeabilization; 6. Preparation of Cytosolic Extract; 7. Lysosomal Cathepsins; 8. Synthetic Cathepsin Substrates
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9. Cleavage of Protein Substrates
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Electronic reproduction; Available via World Wide Web
In:
Pt. A
Weitere Ausg.:
Erscheint auch als Online-Ausgabe Programmed cell death, general principles for studying cell death ; Pt. A Amsterdam [u.a.] : Elsevier, Acad. Press, 2008 ISBN 9780123743121
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Programmed cell death, general principles for studying cell death ; Pt. A Amsterdam [u.a.] : Elsevier, Acad. Press, 2008 ISBN 9780123743121
Sprache:
Englisch
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