UID:
almafu_9958088077102883
Umfang:
1 online resource (742 p.)
Ausgabe:
4th ed.
ISBN:
9786612329982
,
9781282329980
,
1282329987
,
9780080884950
,
0080884954
Inhalt:
This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the b
Anmerkung:
Description based upon print version of record.
,
Front Cover; RNA Methodologies: A Laboratory Guide for Isolation and Characterization; Copyright Page; Contents; Preface; Chapter 1 RNA and the Cellular Biochemistry Revisited; Why Study RNA?; What is RNA?; Assembly of Polynucleotides; Types of RNA; Transcription and the Central Dogma; Important Plant and Animal Models for Studying Transcription; Promoters and Regulatory Elements; Gene and Genome Organization Affect Transcription; RNA Polymerases and the Products of Transcription; Messenger RNA; Topology of a Typical mRNA Molecule; 5' Cap; Leader Sequence; Coding Region; Trailer Sequence
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Poly(A) TailOrganellar mRNAs; mRNA Stability, Transport, and Turnover; Bicistronic mRNAs; Prokaryotic mRNAs; mRNA Sequence and Structure Affect Translation; Levels of Gene Regulation; Alternative Splicing of mRNA from a Single Genetic Locus; Trans-splicing: mRNA Repair; Overview of Small RNAs; miRNA Regulation of Gene Expression; References; Chapter 2 RNA Isolation Strategies; Rationale; Goals in the Purification of RNA; Lysis Buffer Formulations; Gentle Lysis Buffers; Protocol: Isolation of Cytoplasmic RNA by Hypotonic Lysis; Chaotropic Lysis Buffers
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Isolation of RNA with Guanidinium BuffersGuanidinium-Acid-Phenol Extraction Techniques; Protocol: Guanidinium-Acid-Phenol Extraction; Density Gradient Centrifugation; Cesium Chloride; Protocol: Cesium Chloride (CsCl) Gradients; Cesium Trifluoroacetate (CsTFA); Protocol: Cesium Trifluoroacetate (CsTFA) Gradients; Simultaneous Isolation of RNA and DNA; Protocol: Simultaneous Isolation of RNA and DNA; The Word on Kits; Silica Technology; Isolation of Cytoplasmic RNA on a Silica Column; Affinity Matrices; Other Methods; Protocol: Rapid Isolation of RNA with SDS and Potassium Acetate Reagents
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Protocol: Isolation of Prokaryotic RNAProtocol: Isolation of RNA from Yeast; Short- and Long-Term Storage of Purified RNA; References; Chapter 3 The Truth about Tissues; Rationale; Tissue Culture or Tissue?; Advantages of Cell Culture; Advantages of Tissue Samples; Homogenization Methods; Polytron Disruption; Dounce Homogenization; BeadBeaterTM Technique; RNA Isolation Strategies for Various Organs and Tissues; Fresh Tissue; Frozen Tissue; Fixed Tissue; Protocol: LiCl-Urea Method for RNA Isolation from Tissue; Protocol: RNA Isolation from Lipid-Enriched Samples
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Purification of Polysome-Engaged mRNAProtocol: Isolation of Polysomal mRNA; Collecting Samples in the Field; RNA ""Clean-Up"" Methods; Troubleshooting RNA Isolation from Tissue; References; Chapter 4 Going Green: RNA and the Molecular Biology of Plants; Rationale; RNA Isolation and the Peculiarities of Plants; Types of RNA Produced in Plant Cells; Protocol: RNA Isolation from Leaf; Protocol: RNA Isolation from Bark; Protocol: RNA Isolation from Fruit; Strategies for RNA Isolation from Other Plant Tissues; Troubleshooting RNA Isolation from Plant Tissue; References and Suggested Reading
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Chapter 5 Isolation of Polyadenylated RNA
,
English
Weitere Ausg.:
ISBN 9780123747273
Weitere Ausg.:
ISBN 0123747279
Sprache:
Englisch
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