UID:
almafu_9958131518702883
Format:
1 online resource (501 p.)
Edition:
1st ed.
ISBN:
9786612169519
,
9781282169517
,
1282169513
,
9780080956961
,
0080956963
Series Statement:
Methods in enzymology ; v. 461
Content:
The understanding of chemokines, the proteins that control the migration of cells, and their receptors, is critical to the study of causes and therapies for a wide range of human diseases and infections, including certain types of cancer, inflammatory diseases, HIV, and malaria. This volume, focusing on chemokine structure and function, as well as signaling, and its companion volume (Methods in Enzymology volume 461, focusing on chemokines as potential targets for disease intervention) provide a comprehensive overview and time-tested protocols in this field, making it an essential refer
Note:
Description based upon print version of record.
,
Front Cover; Methods in Enzymology: Chemokines, Part B; Copyright Page; Contents; Contributors; Preface; Methods in Enzymology; Chapter 1: Chapter One Isolation, Identification, and Production of Posttranslationally Modified Chemokines; 1. Introduction; 3. Concentration of Isolated Proteins; 6. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE); 10. Edman Degradation; 11. Total Protein Quantification Methods; 14. Citrullination of Chemokines; 15. Identification of Enzymes Generating the Natural Posttranslationally Modified Chemokines, as Exemplified by Aminopeptidase N(A
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16. Comparison of the Heparin-Binding Properties of Chemokine IsoformsChapter 2: Chapter Two Homo- and Hetero-Oligomerization of Chemokines; 1. Introduction; 2. Methods to Detect and Quantify Oligomerization; 3. Methods for Collecting Residue-Specific Information on Chemokine Oligomers; 4. Conclusions; Acknowledgments; Chapter 3: Chapter Three Lymphotactin Structural Dynamics; 1. Introduction; 3. Ltn Folds into Two Unrelated Native State Structures; 4. Kinetics of Interconversion in the Ltn Conformational Equilibrium; 5. Engineering Conformationally Restricted Lymphotactin Variants
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6. Functional Analysis of Distinct Ltn Native State Conformations7. GAG Binding Residues are Linked to the Ltn Conformational Equilibrium; References; Chapter 4: Chapter Four Interactions of Chemokines with Glycosaminoglycans; 1. Introduction; 4. Summary; Acknowledgments; References; Chapter 5: Chapter Five Multiple Approaches to the Study of Chemokine Receptor Homo- and Heterodimerization; 1. Introduction; 3. Resonance Energy Transfer (RET) Techniques; 4. Sequential BRET-FRET (SRET) Technology; 5. Conclusion
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Chapter 6: Chapter Six Plasmon Resonance Methods in Membrane Protein Biology: Applications to GPCR Signaling4. Lipid Bilayer Deposition; 6. Membrane Protein Insertion; 7. Ligand and G-Protein Binding by GPCRs; . Conflicts of Interests; Chapter 7: Chapter 7 Tyrosine Sulfation of HIV-1 Coreceptors and Other Chemokine Receptors; 1. Introduction; 6. Conclusions; Chapter 8: Chapter 8 Activation Mechanisms of Chemokine Receptors; 1. Introduction; 2. Current Models for 7TM Receptor Activation; 3. Constitutive Activity of 7TM Receptors; 4. Activation of Chemokine Receptors by Small-Molecule Agonists
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ReferencesChapter 9: Chapter 9 The Duffy Antigen Receptor for Chemokines; 3. Methods for the Study of DARC as a Chemokine Sink; 6. The Assay of Chemokine Transcytosis by DARC; References; Chapter 10: Chapter Ten Hetero-Oligomerization of Chemokine Receptors; 3. Resonance Energy Transfer Techniques; 4. Developing Techniques; References; Chapter 11: Chapter 11 Subsecond Analyses of G-Protein Coupled-Receptor; 3. Small-Volume Rapid Mix Device Flow Cytometry; 6. Modular Molecular Assemblies of GPCR Ternary Complexes on Beads; Acknowledgments
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Chapter 12: Chapter 12 The Use of Receptor Homology Modeling to Facilitate the Design of Selective Chemokine Receptor Antagonists
Additional Edition:
ISBN 9780123749079
Additional Edition:
ISBN 0123749077
Language:
English
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