UID:
almafu_9958129695902883
Format:
1 online resource (679 p.)
ISBN:
9786612879258
,
9781282879256
,
1282879251
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9780123810021
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0123810027
Series Statement:
Methods in enzymology ; v. 478
Content:
In this 3 volume collection focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers' own research efforts Each chapter has been designed so that enough scientific background will be given in each chapter for further development of methods by readers themselves. Useful for all levels of scientists starting from the last years of colleges, graduate students, postdoctoral fellows to professors and to all levels of scientists in research institutes including industry.
Note:
Description based upon print version of record.
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Front Cover; Methods in Enzymology: Glycomics; Copyright Page; Contents; Contributors; Preface; Methods in Enzymology; Section One: Glycomics Revealed by Mass Spectrometric Analysis; Chapter One: Mass Spectrometric Analysis of Sulfated N- and O-Glycans; 1. Introduction and Overview; 2. Sample Preparation; 3. MS Analyses and Data Interpretation; 4. Future Perspectives; Acknowledgments; References; Chapter Two: Mass Spectrometric Analysis of Mutant Mice; 1. Overview; 2. Methods; 3. Interpretation of Glycomic Data
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4. Example Project: Characterization of Pancreatic Tissue from Wild-Type and Mgat4a Knockout Mice5. Summary of Glycan Structural Observations in Murine Tissues, Cells, and Knockouts; Acknowledgments; References; Chapter Three: Glycosaminoglycan Characterization by Electrospray Ionization Mass Spectrometry Including Fourier Transform Mass S; 1. Overview; 2. Preparation of Crude PG/GAG; 3. Disaccharide Profiling Using Ion-Pairing Reverse-Phase (IP RP) HPLC with MS Detection; 4. CS/DS Disaccharide Preparation; 5. ESI IP RP LC MS Analysis of CS/DS Disaccharides; 6. HS Disaccharide Preparation
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7. ESI IP RP LC MS Analysis of HS Disaccharides8. Direct Infusion ESI FTMS Analysis of Oligosaccharides and Polysaccharides Separated by Preparative Continuous-Elution PAGE.; 9. Preparation of Bikunin GAG; 10. An Approach to FTMS Data Interpretation; 11. Direct Infusion ESI FT-ICR MS Analysis of Bikunin GAG Mixture; 12. Structural Characterization of GAG Oligosaccharides by Tandem Mass Spectrometry; 13. Summary; Acknowledgments; References; Chapter Four: Large-Scale Glycomics for Discovering Cancer-Associated N-Glycans by Integrating Glycoblotting and Mass Spectromet; 1. Introduction
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2. Overview of Glycoblotting Utilized by BlotGlyco BeadsReferences; Chapter Five: In Vitro and In Vivo Enzymatic Syntheses and Mass Spectrometric Database for N-Glycans and O-Glycans; 1. Overview; 2. In Vitro Syntheses of N- and O-Linked Glycan Libraries Using Recombinant Human Glycosyltransferases; 3. In Vivo Production of Mammalian-Type O-Linked Glycopeptides in Yeast; 4. An Application of the Library to Glycan Analysis; 5. Glycan Analysis Using the Glycan Mass Spectral Database ``GMDB ́ ́; 6. Navigator System for Glycan Analysis Using the Glycan Mass Spectral Database; Acknowledgments
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ReferencesSection Two: Glycomics Revealed by Carbohydrate-Binding Proteins; Chapter Six: Identification of Fucosylated Haptoglobin as a Novel Tumor Marker for Pancreatic Cancer and Its Possible Applicati; 1. Introduction; 2. Discovery of Fucosylated Haptoglobin as a Marker for Pancreatic Cancer; 3. Mass Spectrometry Analysis of the Oligosaccharide Structure on Haptoglobin; 4. Identification of an Inducible Factor that Stimulates the Production of Haptoglobin; 5. Clinical Application of Lectin-Antibody ELISA to Measure Fucosylated Haptoglobin; 6. Closing Remarks; Acknowledgments; References
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Chapter Seven: Differential Glycan Profiling by Lectin Microarray Targeting Tissue Specimens
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English
Additional Edition:
ISBN 9780123810014
Additional Edition:
ISBN 0123810019
Language:
English
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