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Umfang: 1 online resource (745 p.)

Ausgabe: 1st ed.

ISBN: 9786612770098 , 9781282770096 , 1282770098 , 9780123810083 , 0123810086

Serie: Methods in cell biology, v. 96

Inhalt: The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and

Anmerkung: Description based upon print version of record. , Methods in Cell Biology; Copyright; Contents; Contributors; Preface; Acknowledgments; Chapter 1. Electron Microscopy of Viruses; I. Introduction; II. Rationale; III. Methods; IV. Instrumentation and Materials; References; Chapter 2. Bacterial TEM: New Insights from Cryo-Microscopy; I. Introduction; II. Methods Involving Dehydration and Metal Stains; III. Cryo-Electron Microscopy; References; Chapter 3. Analysis of the Ultrastructure of Archaea by Electron Microscopy; I. Introduction; II. Growth of Cells for Electron Microscopy; III. Preparation for Transmission Electron Microscopy , IV. Preparation for Scanning Electron MicroscopyV. Instrumentation and Methods; VI. Conclusions; References; Chapter 4. Chlamydomonas: Cryopreparation Methods for the 3-D Analysis of Cellular Organelles; I. Introduction and Historical Perspectives; II. Rationale; III. Methods; IV. Instrumentation and Materials; V. Summary; References; Chapter 5. Ultrastructure of the Asexual Blood Stages of Plasmodium falciparum; I. Introduction; II. Rationale; III. Methods; IV. Materials; References; Chapter 6. Electron Tomography and Immuno-labeling of Tetrahymena thermophila Basal Bodies; I. Introduction , II. RationaleIII. Methods; IV. Instrumentation and Materials; V. Discussion; References; Chapter 7. Electron Microscopy of Paramecium (Ciliata); I. Introduction; II. Methods; III. Final Remarks; References; Chapter 8. Ultrastructural Investigation Methods for Trypanosoma brucei; I. Introduction; II. Rationale; III. Methods; IV. Materials; V. Discussion; VI. Summary; References; Chapter 9. Dictyostelium discoideum: A Model System for Ultrastructural Analyses of Cell Motility and Development; I. Introduction; II. Current Approaches; III. Future Challenges; IV. Summary Comments; References , Chapter 10. Toward Sub-second Correlative Lightand Electron Microscopy of Saccharomyces cerevisiaeI. Introduction; II. Rationale; III. Methods; IV. Materials; V. Results; VI. Discussion; References; Chapter 11. Fission Yeast: A Cellular Model Well Suited for Electron Microscopy Investigations; I. Introduction; II. Rationale; III. EM Methods; IV. Instrumentation, Materials, and Reagents; V. Schizosaccharomyces pombe: Some Major Advances using EM; VI. Discussion and Outlook; VII. Summary; References; Chapter 12. Electron Microscopy and High-Pressure Freezing of Arabidopsis; I. Introduction , II. RationaleIII. Methods; IV. Materials; V. Discussion; VI. Concluding Remarks; References; Chapter 13. Preparation Techniques for Transmission Electron Microscopy of Hydra; I. Introduction; II. Rationale; III. Methods; IV. Materials; V. Results and Discussion; VI. Concluding Remarks; References; Chapter 14. Electron Microscopy of Flatworms: Standard and Cryo-Preparation Methods; I. Introduction; II. Rationale; III. Methods; IV. Materials; V. Results and Discussion; VI. Concluding Remarks; References , Chapter 15. Three-Dimensional Reconstruction Methods for Caenorhabditis elegans Ultrastructure , English

Weitere Ausg.: ISBN 9780123810076

Weitere Ausg.: ISBN 0123810078

Sprache: Englisch