Kooperativer Bibliotheksverbund

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Umfang: 1 online resource (519 p.)

Ausgabe: 1st ed.

ISBN: 9786613920805 , 9781283608350 , 1283608359 , 9780123812735 , 0123812739

Serie: Methods in enzymology, v. 514

Inhalt: This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers ghrelin, and has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice. Contains quality chapters authored by leaders in the field Has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays an

Anmerkung: Description based upon print version of record. , Front Cover; Ghrelin; Copyright; Contents; Contributors; Preface; Methods in Enzymology; Section 1: History of GHS and Ghrelin; Chapter One: History to the Discovery of Ghrelin; 1. Introduction/Pre-GHRP Studies; 2. GHRP Beginning; 3. GHRP-6 Active in Vitro and in Vivo (1982); 4. GHRH Isolation and Identification (1982); 5. GHRP Versus GHRH (1981-1984); 6. GHRP Reflects Activity of a New Hormone and New Receptor-Hypothalamic-Pituitary Models (1984-1991); 7. GHRP/GHS Receptor Assays (1989-2000); 8. GHS/GHRP Receptor (1996-1998); 9. Clinical Results in Humans , 9.1. GHRP-6+GHRH synergistic effect in humans (1989)9.2. 24-h Continuous infusion; 9.3. GHRP administration in children; 9.4. Effect of GHRP-2, GHRH, GHRP-2+GHRH, ghrelin, and ghrelin+GHRH in humans; 10. Summary/Conclusion; Acknowledgments; References; Chapter Two: Orphan GPCRs and Methods for Identifying Their Ligands; 1. Introduction; 2. Strategies for Identifying Ligands of Orphan GPCRs; 3. Selection of Target Orphan GPCRs; 4. Recombinant Expression Systems for Orphan GPCRs; 5. Sources of Potential Ligands of Orphan GPCRs; 5.1. Tissue extracts; 5.2. Bioinformatics , 6. Functional Assays for Screening Candidate Ligands of Orphan GPCRs7. Summary; Acknowledgments; References; Chapter Three: Purification of Rat and Human Ghrelins; 1. Introduction; 2. GHS and its Receptor; 2.1. History; 2.2. The GHS-R superfamily; 3. Purification of Rat Ghrelin; 3.1. Construction of GHS-R-expressing cells; 3.1.1. Construction of the GHS-R-expressing cell line; 3.2. Monitoring intracellular calcium via a fluorometric imaging plate reader-based assay; 3.2.1. FLIPR assay; 3.3. Purification of rat ghrelin; 3.3.1. Purification of ghrelin from rat stomach tissue , 3.4. Structural determination of rat ghrelin4. Purification of Human Ghrelin; 4.1. Purification of human ghrelin from stomach tissue; 4.2. Mass spectrometric analysis of human ghrelins; 4.3. Molecular forms of human ghrelin; 5. Purification of Rat des-Gln14-ghrelin; 5.1. Purification of des-Gln14-ghrelin from rat stomach tissue; 6. Obestatin, an Anorexigenic Hormone or a Digested Peptide Fragment from a Ghrelin Precursor?; 7. Conclusion; References; Chapter Four: Variety of Acyl Modifications in Mammalian Ghrelins; 1. Introduction; 2. Purification of Mammalian Ghrelin , 2.1. Purification of feline ghrelin2.1.1. Calcium mobilization assays; 2.1.2. Purification of feline ghrelin; 2.1.3. Cloning of feline ghrelin cDNA; 2.1.4. Structural determination of feline ghrelin; 2.2. Purification of caprine ghrelin; 3. GH-Releasing Activity of Ghrelin in Mammals; 3.1. GH-releasing activity of ghrelin in cats; 3.1.1. Preparation of plasma samples; 3.1.2. Radioiodination of GH; 3.1.3. Radioimmunoassay of GH; 3.2. GH-releasing activity of ghrelin in goats; 4. Summary; Acknowledgments; References; Chapter Five: Determination of Nonmammalian Ghrelin; 1. Introduction , 2. Purification of Ghrelin , English

Weitere Ausg.: ISBN 9780123812728

Weitere Ausg.: ISBN 0123812720

Sprache: Englisch

UID:

Umfang: LVII, 452 S., [8] Taf. , Ill.

Ausgabe: 1. ed.

Ausgabe: Online-Ausg. [S.l.] Elsevier Online-Ressource

ISBN: 9780123812728 , 0123812720

Serie: Methods in enzymology 514

Inhalt: This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers ghrelin, and has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice. Contains quality chapters authored by leaders in the field Has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice

Anmerkung: Includes bibliographical references and indexes. - Print version record , History to the discovery of ghrelin / Cyril Y. BowersOrphan GPCRs and methods for identifying their ligands / Morikatsu Yoshida, Mikiya Miyazato, and Kenji Kangawa -- Purification of rat and human ghrelins / Masayasu Kojima, Hiroshi Hosoda, and Kenji Kangawa -- Variety of acyl modifications in mammalian ghrelins / Takanori Ida -- Determination of nonmammalian ghrelin / Hiroyuki Kaiya [and others] -- Morphological analysis of ghrelin neurons in the hypothalamus / Haruaki Kageyama, Fumiko Takenoya, and Seiji Shioda -- High-performance liquid chromatography analysis of hypothalamic ghrelin / Takahiro Sato and Masayasu Kojima -- Standard sample collections for blood ghrelin measurements / Hiroshi Hosoda and Kenji Kangawa -- From ghrelin to ghrelin's O-acyl transferase / Jesus A. Gutierrez [and others] -- Enzymatic characterization of GOAT, ghrelin O-acyltransferase / Hideko Ohgusu, Tomoko Takahashi, and Masayasu Kojima -- The study of ghrelin deacylation enzymes / Motoyasu Satou and Hiroyuki Sugimoto -- Synthesis of ghrelin : chemical synthesis and semisynthesis for large-scale preparation of modified peptides / Tomohiro Makino, Masaru Matsumoto, and Yoshiharu Minamitake -- Ghrelin O-acyltransferase assays and inhibition / Martin S. Taylor [and others] -- Model-based evaluation of growth hormone secretion / Johannes D. Veldhuis and Daniel M. Keenan -- Ghrelin in the control of energy, lipid, and glucose metabolism / Kristy M. Heppner [and others] -- Ghrelin and the vagus nerve / Yukari Date -- Measurement of AMP-activated protein kinase activity and expression in response to ghrelin / Chung Thong [and others] -- Ghrelin and gastrointestinal movement / Naoki Fujitsuka [and others] -- Ghrelin acylation by ingestion of medium-chain fatty acids / Yoshihiro Nishi, Hiroharu Mifune, and Masayasu Kojima -- Islet [beta]-cell ghrelin signaling for inhibition of insulin secretion / Katsuya Dezaki and Toshihiko Yada -- Rikkunshito as a ghrelin enhancer / Hiroshi Takeda [and others] -- Thermogenic characterization of ghrelin receptor null mice / Ligen Lin and Yuxiang Sun -- Transgenic mice overexpressing ghrelin or ghrelin analog / Hiroyuki Ariyasu [and others] -- Therapeutic potential of ghrelin in restricting-type anorexia nervosa / Mari Hotta [and others] -- Clinical application of ghrelin for chronic respiratory diseases / Nobuhiro Matsumoto and Masamitsu Nakazato -- Clinical trial of ghrelin synthesis administration for upper GI surgery / Shuji Takiguchi [and others].

Sprache: Englisch

Fachgebiete: Biologie

Schlagwort(e): Electronic books

URL: Volltext

URL: Volltext

URL: Volltext

URL: Volltext

UID:

Umfang: 1 Online-Ressource

Ausgabe: 1. ed.

ISBN: 9780123812728 , 9780123812735

Serie: Methods in enzymology 514

Sprache: Englisch

Fachgebiete: Biologie

Schlagwort(e): Ghrelin ; Molekularbiologische Methode ; Aufsatzsammlung

URL: Volltext