UID:
almafu_9958127188202883
Umfang:
1 online resource (519 p.)
Ausgabe:
1st ed.
ISBN:
9786613920805
,
9781283608350
,
1283608359
,
9780123812735
,
0123812739
Serie:
Methods in enzymology, v. 514
Inhalt:
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers ghrelin, and has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice. Contains quality chapters authored by leaders in the field Has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays an
Anmerkung:
Description based upon print version of record.
,
Front Cover; Ghrelin; Copyright; Contents; Contributors; Preface; Methods in Enzymology; Section 1: History of GHS and Ghrelin; Chapter One: History to the Discovery of Ghrelin; 1. Introduction/Pre-GHRP Studies; 2. GHRP Beginning; 3. GHRP-6 Active in Vitro and in Vivo (1982); 4. GHRH Isolation and Identification (1982); 5. GHRP Versus GHRH (1981-1984); 6. GHRP Reflects Activity of a New Hormone and New Receptor-Hypothalamic-Pituitary Models (1984-1991); 7. GHRP/GHS Receptor Assays (1989-2000); 8. GHS/GHRP Receptor (1996-1998); 9. Clinical Results in Humans
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9.1. GHRP-6+GHRH synergistic effect in humans (1989)9.2. 24-h Continuous infusion; 9.3. GHRP administration in children; 9.4. Effect of GHRP-2, GHRH, GHRP-2+GHRH, ghrelin, and ghrelin+GHRH in humans; 10. Summary/Conclusion; Acknowledgments; References; Chapter Two: Orphan GPCRs and Methods for Identifying Their Ligands; 1. Introduction; 2. Strategies for Identifying Ligands of Orphan GPCRs; 3. Selection of Target Orphan GPCRs; 4. Recombinant Expression Systems for Orphan GPCRs; 5. Sources of Potential Ligands of Orphan GPCRs; 5.1. Tissue extracts; 5.2. Bioinformatics
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6. Functional Assays for Screening Candidate Ligands of Orphan GPCRs7. Summary; Acknowledgments; References; Chapter Three: Purification of Rat and Human Ghrelins; 1. Introduction; 2. GHS and its Receptor; 2.1. History; 2.2. The GHS-R superfamily; 3. Purification of Rat Ghrelin; 3.1. Construction of GHS-R-expressing cells; 3.1.1. Construction of the GHS-R-expressing cell line; 3.2. Monitoring intracellular calcium via a fluorometric imaging plate reader-based assay; 3.2.1. FLIPR assay; 3.3. Purification of rat ghrelin; 3.3.1. Purification of ghrelin from rat stomach tissue
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3.4. Structural determination of rat ghrelin4. Purification of Human Ghrelin; 4.1. Purification of human ghrelin from stomach tissue; 4.2. Mass spectrometric analysis of human ghrelins; 4.3. Molecular forms of human ghrelin; 5. Purification of Rat des-Gln14-ghrelin; 5.1. Purification of des-Gln14-ghrelin from rat stomach tissue; 6. Obestatin, an Anorexigenic Hormone or a Digested Peptide Fragment from a Ghrelin Precursor?; 7. Conclusion; References; Chapter Four: Variety of Acyl Modifications in Mammalian Ghrelins; 1. Introduction; 2. Purification of Mammalian Ghrelin
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2.1. Purification of feline ghrelin2.1.1. Calcium mobilization assays; 2.1.2. Purification of feline ghrelin; 2.1.3. Cloning of feline ghrelin cDNA; 2.1.4. Structural determination of feline ghrelin; 2.2. Purification of caprine ghrelin; 3. GH-Releasing Activity of Ghrelin in Mammals; 3.1. GH-releasing activity of ghrelin in cats; 3.1.1. Preparation of plasma samples; 3.1.2. Radioiodination of GH; 3.1.3. Radioimmunoassay of GH; 3.2. GH-releasing activity of ghrelin in goats; 4. Summary; Acknowledgments; References; Chapter Five: Determination of Nonmammalian Ghrelin; 1. Introduction
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2. Purification of Ghrelin
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English
Weitere Ausg.:
ISBN 9780123812728
Weitere Ausg.:
ISBN 0123812720
Sprache:
Englisch
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