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Format: 1 online resource (411 p.)

ISBN: 9786612738050 , 9781282738058 , 1282738054 , 9780123813466 , 0123813468

Series Statement: Methods in enzymology, v. 473

Content: This volume, along with its companion (volume 475), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. This first installment of Cadenas and Packer's two-volume treatment specifically deals with glutathionylation and dethiolation, and peroxide removal by peroxiredoxins/thioredoxins and glutathione peroxidases. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has bee

Note: Description based upon print version of record. , Front Cover; Methods in Enzymology: Thiol Redox Transitions in Cell Signaling, Part A; Copyright Page; Contents; Contributors; Preface; Volumes in Series; Chapter 1: Changing Paradigms in Thiology: From Antioxidant Defense Toward Redox Regulation; 1. Introduction; 2. Early Concepts, Misconceptions, Unsettled Battles, and Persistent Confusion; 3. The Discovery of ""Natural"" Free Radicals; 4. Love Affairs Between Hydroperoxides and Thiolates or Selenolates; 4.1. Glutathione peroxidases; 4.2. Peroxiredoxins; 4.3. Other thiol peroxidases; 4.4. Redoxins , 5. Toward Regulatory Circuits with Puzzle Stones from Redox Biochemistry5.1. Triggering signals; 5.2. Hydroperoxide sensors; 5.3. Signal transducers; 5.4. Targets; 5.5. Shut-off switches and restoration of starting conditions; 5.6. Signaling versus defense or modulation of redox signaling by competition; 6. Conclusions and Perspectives; References; Chapter 2: Mass Spectrometry-Based Methods for the Determination of Sulfur and Related Metabolite Concentrations in Cell Extracts; 1. Introduction; 2. Analytical Methods for the Determination of Sulfur and Amino Acid Metabolites; 3. Procedures , 3.1. Cell growth and 15N metabolic labeling3.2. Metabolite extraction; 4. Absolute LC-ESI-MS/MS Quantification of Thiol and Amino Acid Metabolites in Yeast Extracts; 4.1. Sample preparation; 4.2. Liquid chromatography; 4.3. Mass spectrometry; 4.4. Data processing; 4.5. Method validation; 5. Qualitative and Quantitative Determination of Thiol and Amino Acid Metabolites in Yeast Extracts by Using an LTQ-Orbitrap Mass Spectrometer; 5.1. Sample preparation; 5.2. Direct introduction; 5.3. LC/MS; 5.4. Data processing; 6. Discussion; 7. Summary; Acknowledgments; References , Chapter 3: Use of Dimedone-Based Chemical Probes for Sulfenic Acid Detection: Evaluation of Conditions Affecting Probe Incorporation into Redox-Sensitive Proteins1. Introduction; 2. Materials; 2.1. Solutions; 2.2. Chemical modification agents; 2.3. Proteins; 3. Methods; 3.1. Characterization of ""DCP""-linked compounds; 3.2. Protocols for labeling cysteine sulfenic acids within cellular proteins; 4. Summary; Acknowledgments; References; Chapter 4: Use of Dimedone-Based Chemical Probes for Sulfenic Acid Detection: Methods to Visualize and Identify Labeled Proteins; 1. Introduction , 2. Biotin-Based Affinity Capture to Identify Proteins Containing Cysteine Sulfenic Acids2.1. Materials; 2.2. Methods; 3. Detection Methods to Identify Oxidized Proteins and Cysteines; 3.1. Targeted approaches: Western blot of affinity-enriched proteins to analyze proteins of interest; 3.2. Targeted approaches: Immunoprecipitation of protein of interest followed by Western blot to detect biotin; 3.3. Controls for endogenous biotinylation; 3.4. Global approaches: Identification of overall sulfenic acid levels for cellular proteins in response to stimuli , 3.5. Global approaches: Identification of oxidized proteins by mass spectrometry after biotin affinity capture , English

Additional Edition: ISBN 9780123813459

Additional Edition: ISBN 012381345X

Language: English

UID:

Format: 1 Online-Ressource

Edition: 1. ed.

ISBN: 9780123813459

Series Statement: Methods in enzymology 473

In: 1

Language: English

Subjects: Chemistry/Pharmacy , Biology

Keywords: Thiole ; Labortechnik

URL: Volltext

UID:

Format: Online-Ressource (359 S.)

Edition: 1. ed.

Edition: Online-Ausg. 2011 Electronic reproduction; Available via World Wide Web

ISBN: 9780123813459

Series Statement: Thiol redox transitions in cell signaling / ed. by Enrique Cadenas and Lester Packer Pt. A

Content: This volume, along with its companion (volume 474), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. This first installment of Cadenas and Packer's two-volume treatment specifically deals with glutathionylation and dethiolation,and peroxide removal by peroxiredoxins/thioredoxins and glutathione peroxidases. The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has ...

Note: Includes bibliographical references and indexes , Changing paradigms in thiology: from antioxidant defense toward redox regulation / Leopold FlohéMass spectrometry-based methods for the determination of sulfur and related metabolite concentrations in cell extracts / Emmanuel Godat ... [et al.] -- Use of dimedone-based chemical probes for sulfenic acid detection: evaluation of conditions affecting probe incorporation into redox-sensitive proteins / Chananat Klomsiri ... [et al.] -- Use of dimedone-based chemical probes for sulfenic acid detection: methods to visualize and identify labeled proteins / Kimberly J. Nelson ... [et al.] -- Formation and reactions of sulfenic acid in human serum albumin / Beatriz Alvarez ... [et al.] -- Determination of GSH, GSSG, and GSNO using HPLC with electrochemical detection / Li-Peng Yap ... [et al.] -- Measurement of mixed disulfides including glutathionylated proteins / Raffaella Priora ... [et al.] -- Detection and quantification of protein disulfides in biological tissues: a fluorescence-based proteomic approach / Viviana I. Pérez ... [et al.] -- Measurement and identification of S-glutathiolated proteins / Bradford G. Hill ... [et al.] -- Proteome screens for Cys residues oxidation: the redoxome / Giovanni Chiappetta ... [et al.] -- Identification by MS/MS of disulfides produced by a functional redox transition / Pierluigi Mauri ... [et al.] -- Mass spectrometry approaches for the redox characterization of protein cysteine residues: the case of the transcription factor Pax-8 / Andrea Scaloni and Gianluca Tell -- A simple method to systematically study oxidatively modified proteins in biological samples and its applications / Byoung-Joon Song, Soo-Kyung Suh and Kwan-Hoon Moon -- Direct and indirect detection methods for the analysis of S-nitrosylated peptides and proteins / Federico Torta, Lisa Elviri and Angela Bachi -- A rapid approach for the detection, quantification, and discovery of novel sulfenic acid or S-nitrosothiol modified proteins using a biotin-switch method / Joseph R. Burgoyne and Philip Eaton -- Protein addicts of aldehydic lipid peroxidation products: identification and characterization of protein adducts using an aldehyde/keto-reactive probe in combination with mass spectrometry / Claudia S. Maier ... [et al.]. , Electronic reproduction; Available via World Wide Web

Additional Edition: Erscheint auch als Druck-Ausgabe Methods in enzymology Thiol Redox Transitions in Cell Signaling : Part A : Chemistry and Biochemistry of Low Molecular Weight and Protein Thiols

Language: English

URL: Volltext