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Umfang: 1 online resource (549 p.)

ISBN: 9786612879395 , 9781282879393 , 1282879391 , 9780123813503 , 0123813506

Serie: Methods in cell biology, v. 97

Inhalt: This volume includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation. Iincludes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to stu

Anmerkung: Description based upon print version of record. , Cover; Methods in Cell Biology; Copyright; Contents; Contributors; Preface; 1. Determination of Microtubule Dynamic Instability in Living Cells; I. Introduction; II. Methods and Materials; III. Discussion and Summary; References; 2. Analysis of Microtubule Polymerization Dynamics in Live Cells; I. Introduction; II. Rationale; III. Imaging and Analysis of Homogeneously Labeled MTs; IV. MT Fluorescent Speckle Microscopy; V. Imaging and Analysis of Growing MT Ends; VI. Conclusion; References , 3. The Use of Fluorescence Redistribution After Photobleaching for Analysisof Cellular Microtubule DynamicsI. Introduction; II. Choice and Preparation of Cells; III. Maintaining Cell Viability While Imaging; IV. Imaging and Data Analysis; V. Summary and Conclusions; Reference; 4. Kinetochore-Microtubule Dynamics and Attachment Stability; I. Introduction; II. Materials; III. Methods; IV. Summary and Conclusions; References; 5. Photoactivatable Green Fluorescent Protein-Tubulin; I. Introduction; II. Conclusions; References; 6. Microtubule Dynamics at the Cell Cortex Probed by TIRF Microscopy , I. IntroductionII. Rationale; III. Materials and Equipment; IV. Methods; V. Discussion; VI. Summary; References; 7. Microtubule Dynamics in Dendritic Spines; I. Introduction; II. Rationale; III. Culturing Primary Hippocampal Neurons; IV. Expression of EB3-GFP in Hippocampal Neurons UsingLipophilic Transfection; V. Expression of EB3-GFP in Hippocampal Neurons Using SFV; VI. Imaging EB3-GFP by TIRF and Spinning Disk Microscopy; VII. Data Analysis; VIII. Conclusion; References; 8. Protein Micropatterns: A Direct Printing Protocol Using Deep UVs; I. Introduction; II. Designing a Photomask , III. Micropatterned Substrate FabricationIV. Cell Deposition; V. Discussion; VI. General Conclusions; References; 9. New and Old Reagents for Fluorescent Protein Tagging of Microtubulesin Fission Yeast: Experimental and Critical Evaluation; I. Introduction; II. Which GFP-Tubulin Should I Use?; III. Searching for the "GFP" of RFPs; IV. Generation and Evaluation of New RFPs in Fission Yeast; V. The Hunt for Red Tubulin; VI. Successful Fluorescent Imaging of Fission Yeast Microtubules andAssociated Proteins; References; 10. Optical Trapping and Laser Ablation of Microtubules in Fission Yeast , I. IntroductionII. Optical Manipulation; III. Optical Tweezing in Fission Yeast; IV. Laser Ablation of Microtubules; V. Methods; References; 11. A Fast Microfluidic Temperature Control Device for Studying MicrotubuleDynamics in Fission Yeast; I. Introduction; II. Device and Setup Presentation; III. Mold and Device Fabrication; IV. Setup Installation; V. Biological Experiments; VI. Conclusion; VII. Materials; References; 12. Microtubule-Dependent Spatial Organization of Mitochondria in Fission Yeast; I. Introduction; II. Visualization of Mitochondria in Fission Yeast , III. Functional Analysis of MT-Mitochondria Interactionin Live Cells , English

Weitere Ausg.: ISBN 9780123813497

Weitere Ausg.: ISBN 0123813492

Sprache: Englisch