Umfang:
Online-Ressource (XLVII, 399 S., [8] Bl.]
Ausgabe:
1. ed.
Ausgabe:
Online-Ausg. 2011 Electronic reproduction; Available via World Wide Web
ISBN:
9780123813794
Serie:
Methods in enzymology 465
Inhalt:
Liposomes are cellular structures made up of lipid molecules, which are water insoluable organic molecules and the basis of biological membranes. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes Partnbsp,F is a continuation of previous MIE Liposome volumes A through E. * One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences
Anmerkung:
Includes bibliographical references and index
,
Front Cover; Methods in Enzymology; Copyright Page; Contents; Contributors; Preface; Methods in Enzymology; Section 1: Advances in Liposome Formation and Characterization; Chapter 1: Spontaneously Formed Unilamellar Vesicles; 1. Introduction; 2. Preparation of Spontaneously Forming ULVs; 3. Characterization of ULVs; 4. ULV Stability; 5. Parameters Affecting ULVs; 6. Mechanism of ULV Formation; 7. Encapsulation and Controlled Release Mechanism of Spontaneously Formed ULVs; 8. Application; 9. Concluding Remarks; Acknowledgment; References
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Chapter 2: Use of Acoustic Sensors to Probe the Mechanical Properties of Liposomes1. Introduction; 2. Acoustic Measurements; 3. Experimental Procedures; 4. Change in DeltaPh and DeltaA for Adsorbed Vesicles as a Function of Cholesterol Content; 5. Comparison of the Results to Various Model Systems; 6. Can the Response Be Explained in Terms of Variable Slip at the Surface?; 7. Displacements Associated with the Liposomes; 8. Concluding Remarks; Acknowledgments; References; Chapter 3: Liposome Characterization by Quartz Crystal Microbalance Measurements and Atomic Force Microscopy
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1. Introduction2. Liposomes Assessed by AFM; 3. QCM Measurements of Intact Liposomes; 4. Surface-Bound Liposomes in Perspective and General Conclusions; References; Chapter 4: Mixing Solutions in Inkjet Formed Vesicles; 1. Introduction; 2. Unilamellar Vesicle Formation by Microfluidic Encapsulation; 3. Determination of Encapsulation Fraction within Vesicles; 4. Concluding Remarks; References; Chapter 5: Recombinant Proteoliposomes Prepared Using Baculovirus Expression Systems; 1. Introduction; 2. Principles of Recombinant Proteoliposome Preparation; 3. Construction of Recombinant AcNPVs
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4. Expression of Recombinant Proteins on BV Envelopes5. Preparation of Proteoliposomes by Fusion of BVs with Liposomes; 6. Concluding Remarks; References; Chapter 6: Block Liposomes: Vesicles of Charged Lipids with Distinctly Shaped Nanoscale Sphere-, Pear-, Tube-, or Rod-Segments; 1. Introduction; 2. Liposome Preparation; 3. Microscopy; 4. Design and Synthesis of MVLBG2; 5. Phase Behavior of MVLBG2/DOPC Lipid Mixtures; 6. Concluding Remarks; Acknowledgments; References; Chapter 7: Microfluidic Methods for Production of Liposomes; 1. Introduction
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2. Conventional Technologies for Production of Liposomes3. Microfluidic Technologies for Synthesis of Nanoparticles; 4. Microfluidic Technologies for Production of Liposomes; 5. Concluding Remarks; Acknowledgments; References; Chapter 8: Constructing Size Distributions of Liposomes from Single-Object Fluorescence Measurements; 1. Introduction; 2. How Particle Size can be Obtained from Intensity Distributions; 3. Vesicle Preparation and Immobilization; 4. Image Acquisition; 5. Image Processing; 6. Intensity and Size Distributions; 7. Multilamellarity Assay
,
8. Measuring Membrane-Curvature Selective Protein Binding
,
Electronic reproduction; Available via World Wide Web
In:
Pt. G
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Liposomes ; Pt. G Amsterdam [u.a.] : Elsevier, Acad. Press, 2009 ISBN 9780123813794
Sprache:
Englisch
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