UID:
almafu_9958130970802883
Umfang:
1 online resource (363 p.)
Ausgabe:
3rd ed.
ISBN:
9786612955105
,
9781282955103
,
1282955101
,
9780123848932
,
0123848938
Serie:
Methods in cell biology ;
Inhalt:
This volume of Methods in Cell Biology, the first of 3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases.* Details state-of-the art zebrafi
Anmerkung:
Description based upon print version of record.
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Cover; Methods in Cell Biology; Copyright; Dedication; Tableofcontents; Contributors; Preface; Part I Cellular Biology; Analyzing Retinal Axon Guidance in Zebrafish; Introduction; Visualizing Retinal Axons; Perturbing the Retinotectal System; Future Directions; References; Imaging Blood Vessels in the Zebrafish; Introduction; Imaging Vascular Gene Expression; Non-vital Blood Vessel Imaging; Vital Imaging of Blood Vessels; Conclusion; References; Medaka Haploid Embryonic Stem Cells; Introduction; Methods; Applications of Haploid ES Cells; Summary; References
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Part II Developmental and Neural BiologyNeurogenesis; Introduction; Neurogenesis During Development and in the Adult Brain; Methods for Studying Neurogenesis in the Developing and Adult Brain; Specific Protocols to Study Adult Neurogenesis; Conclusion; References; Studying Peripheral Sympathetic Nervous System Development and Neuroblastoma in Zebrafish; Introduction; The Peripheral Autonomic Nervous System; The Zebrafish as a Model System for Studying PSNS Development; Zebrafish as a Novel Model for Studying Neuroblastoma; Conclusion and Future Directions; Acknowledgments; References
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Analysis of the Retina in the Zebrafish ModelIntroduction; Development of the Zebrafish Retina; Analysis of Wild-Type and Mutant Visual System; Analysis of Gene Function in the Zebrafish Retina; Summary; References; Photoreceptor Structure and Development: Analyses using GFP Transgenes; Introduction; Transport Mechanisms; Regulation of Photoreceptor Size; Photoreceptor Synapse Structure; Regeneration; Future Directions; Conclusions; References; Physiological Recordings from Zebrafish Lateral-Line Hair Cells and Afferent Neurons; Introduction; Zebrafish Mounting and Immobilizing; Microphonics
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Action CurrentsSummary; Discussion; References; Zebrafish Kidney Development; Introduction; Structure of the Zebrafish Pronephros; Formation of the Pronephros; Methods to Study Pronephros Function; Conclusions; References; Molecular Regulation of Pancreas Development in Zebrafish; Introduction; Pancreas Development; Analysis of Beta-Cell Migration and Proliferation; Future Directions; References; Monitoring Sleep and Arousal in Zebrafish; Introduction; Behavior, Genetics, and Pharmacology of Zebrafish Sleep; Methods for Monitoring Sleep/Wake Behavior in Zebrafish; Conclusion; References
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Use of Flatbed Transparency Scanners in Zebrafish Research: Versatile and Economical Adjuncts to Traditional Imaging Tools for the Danio rerio LaboratoryIntroduction; Scanner Basics; Motility Analysis; Oocyte and Egg Assays; Using Scanners to Count and Measure; Other Potential Applications of Scanners; Summary: Inexpensive Adjunct to Microscopy; References; Subject Index; Volumes in Series
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English
Weitere Ausg.:
ISBN 9780123848925
Weitere Ausg.:
ISBN 012384892X
Sprache:
Englisch
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