Umfang:
Online-Ressource (XLVII, 384 S.)
,
Ill., graph. Darst.
Ausgabe:
Online-Ausg. 2011 Electronic reproduction; Available via World Wide Web
ISBN:
9780123851147
Serie:
Methods in enzymology 490
Inhalt:
This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.
Inhalt:
This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. 〈br〉〈br〉 This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights
Anmerkung:
Includes bibliographical references and indexes
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Front Cover; Methods in Enzymology: The Unfolded Protein Response and Cellular Stress, Part B; Copyright; Contents; Contributors; Preface; Methods in Enzymology; Chapter 1: Methods for Investigating the UPR in Filamentous Fungi; 1. Introduction; 2. Conditions to Study the UPR in Filamentous Fungi; 3. Analysis of Expression During the ER Stress; 4. Modifying the UPR Signaling by Targeted Gene Replacement; Acknowledgments; References; Chapter 2: Assays for Detecting the Unfolded Protein Response; 1. Introduction; 2. Experimental Approaches for the Detection of ER Stress; 3. Concluding Remarks
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AcknowledgmentsReferences; Chapter 3: Analysis of the Role of Nerve Growth Factor in Promoting Cell Survival During Endoplasmic Reticulum Stress in PC12; 1. Introduction; 2. Induction of ER Stress in PC12 Cells; 3. Measurement of Caspase-3 Activity; 4. Fragmentation of Caspase-12; 5. Hyperinduction of GRP78 by NGF; Acknowledgments; References; Chapter 4: Measuring ER Stress and the Unfolded Protein Response Using Mammalian Tissue Culture System; 1. Introduction; 2. ER Stress and the UPR; 3. Cell Culture System and ER Stress Induction; 4. Measuring ER Stress; 5. Studying the UPR Activation
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6. Studying UPR Downstream Markers and ResponsesAcknowledgments; References; Chapter 5: Real-Time Monitoring of ER Stress in Living Cells and Animals Using ESTRAP Assay; 1. Introduction; 2. SEAP Reporter System; 3. Monitoring of ER Stress in Culture Cells by ESTRAP; 4. Monitoring of ER Stress In Vivo by ESTRAP; 5. Conclusion; References; Chapter 6: HIV Protease Inhibitors Induce Endoplasmic Reticulum Stress and Disrupt Barrier Integrity in Intestinal Epithelial Cells; 1. The Pathophysiological Relevance of HIV PI-Induced ER Stress and UPR Activation in IECs
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2. Analysis of HIV PI-Induced ER Stress and UPR Activation in Cultured IECs3. Analysis of HIV PI-Induced ER Stress and Disruption of Barrier Integrity in the Intestine; 4. Summary; Acknowledgments; References; Chapter 7: Dexamethasone Induction of a Heat Stress Response; 1. Introduction; 2. Evaluation of the Heat Stress Response in Patients Treated with Dexamethasone; 3. Heat Stress Response and UPR in Mice and Cellular Models; References; Chapter 8: Detecting and Quantitating Physiological Endoplasmic Reticulum Stress; 1. Introduction; 2. Detecting UPR at the Level of UPR Sensors
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3. Quantitating ER Stress at the Level of UPR Sensors4. Detecting Levels of Other Common UPR Targets; 5. Important Tips; 6. Concluding Remarks; Acknowledgments; References; Chapter 9: PI 3-Kinase Regulatory Subunits as Regulators of the Unfolded Protein Response; 1. The Unfolded Protein Response; 2. PI 3-Kinase Regulatory Subunits as Modulators of the UPR; 3. Assessing UPR Pathway Activation; References; Chapter 10: The Emerging Role of Histone Deacetylases (HDACs) in UPR Regulation; 1. HDAC Enzymes and Cancer; 2. HDAC Inhibitors; 3. HDAC Inhibition and the UPR; 4. Future Directions
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References
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Electronic reproduction; Available via World Wide Web
In:
Pt. B
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Methods in enzymology The Unfolded Protein Response and Cellular Stress, Part B
Sprache:
Englisch
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