Format:
Online-Ressource (XLVII, 370 S.)
,
Ill., graph. Darst
Edition:
Online-Ausg. 2011 Electronic reproduction; Available via World Wide Web
ISBN:
9780123851161
Series Statement:
Methods in enzymology 489
Content:
This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.
Content:
This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. 〈br〉〈br〉 This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights
Note:
Literaturangaben
,
Front Cover; Methods in Enzymology: The Unfolded Protein Response and Cellular Stress, Part A; Copyright; Contents; Contributors; Preface; Methods in Enzymology; Section 1: UPR and Cell Stress in Normal and Diseased Cells; Chapter 1: The Unfolded Protein Response in Mouse Cerebral Cortex; 1. Introduction: The UPR; 2. Transduction of the UPR Signal by PERK, IRE1, and ATF6; 3. UPR and Apoptosis; 4. The UPR in Health and Disease; 5. Induction of the UPR by Sleep Deprivation; 6. Experimental Protocols; 7. Concluding Remarks; Acknowledgments; References
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Chapter 2: Immunohistochemical Detection of the Unfolded Protein Response in Atherosclerotic Plaques1. Endoplasmic Reticulum and the Unfolded Protein Response; 2. Atherosclerosis; 3. UPR in the Atherosclerotic Lesion; 4. Mouse Models of Atherosclerosis; 5. Methods; Acknowledgments; References; Chapter 3: The Role of PDI as a Survival Factor in Cardiomyocyte Ischemia; 1. Introduction; 2. Identification of PDI as Cardio-Protective Factor; 3. Conclusions; References; Chapter 4: Measurement of the Increase in Endoplasmic Reticulum Stress-Related Proteins and Genes in Adipose Tissue of Obese,
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1. Introduction2. Study Subjects and Fat Biopsies; 3. Proteomic Analysis; 4. Western Blot Analysis; 5. RT-PCR Analysis; Acknowledgments; References; Chapter 5: Measurement of the Unfolded Protein Response (UPR) in Monocytes; 1. Introduction; 2. Investigating UPR Activation in the Monocyte; Acknowledgments; References; Chapter 6: Assessment of Endurance Exercise Training in Hepatic Endoplasmic Reticulum Stress; 1. Introduction; 2. Assessment of an Endurance-Training Program; 3. ""Dietary-Inducible"" Hepatic ER Stress; 4. Assessment of Hepatic ER Stress Molecular Markers; 5. Required Materials
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AcknowledgmentsReferences; Chapter 7: Discovery Approaches to UPR in Athero-Susceptible Endothelium In Vivo; 1. Introduction; 2. Procedures for the Isolation of Endothelial Cells and Preparation of RNA for Microarray Hybridization; 3. Microarray Hybridization and Feature Extraction; 4. Bioinformatics Analysis; 5. Another Approach to Gene Connectivity: Weighted Gene Coexpression Network Analysis; 6. Validation and Follow-up; Acknowledgments; References; Chapter 8: Assessment of Endoplasmic Reticulum Stress and the Unfolded Protein Response in Endothelial Cells; 1. Introduction
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2. The Acute Phase of ER Stress3. The Adaptive Reaction Phase: Unfolded Protein Response in Endothelial Cells; 4. The Final Phase: Cell Death Signaling; Acknowledgments; References; Chapter 9: Measuring the Impact of Cigarette Smoke on the UPR; 1. Introduction; 2. Preparing Samples for Analysis; 3. Analysis of Activation of the PERK Pathway; 4. ATF6 Pathway; 5. IRE1 Pathway; 6. Using LSC to Measure UPR Activation; Acknowledgments; References; Chapter 10: Induction and Measurement of UPR and Osmotic Stress in the Yeast Pichia pastoris; 1. Introduction; 2. Measuring UPR Induction in P. pastoris
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3. Inducing and Measuring Osmotic Stress in P. pastoris
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Electronic reproduction; Available via World Wide Web
In:
Pt. A
Additional Edition:
Erscheint auch als Druck-Ausgabe Methods in enzymology The Unfolded Protein Response and Cellular Stress, Part A
Language:
English
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