Umfang:
Online-Ressource (XLIX, 524 S.)
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Ill., graph. Darst.
Ausgabe:
Online-Ausg. 2011 Electronic reproduction; Available via World Wide Web
ISBN:
9780123864895
Serie:
Methods in enzymology 496
Inhalt:
The global nitrogen cycle is the one most impacted by mankind. The past decade has changed our view on many aspects of the microbial biogeochemical cycles including the global nitrogen cycle, which is mainly due to tremendous advances in methods, techniques and approaches. Many novel processes and the molecular inventory and organisms that facilitate them have been discovered only within the last 5 to 10 years, and the process is in progress. This volume provides state-of-the-art updates on methods and protocols dealing with the detection, isolation and characterization of macromolecules and their hosting organisms that facilitate nitrification and related processes in the nitrogen cycle as well as the challenges of doing so in very diverse environments. Provides state-of-the-art update on methods and protocols Deals with the detection, isolation and characterization of macromolecules and their hosting organismsnbsp, deals with the challenges ofnbsp,very diverse environments.
Anmerkung:
Literaturangaben
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Front Cover; Research on Nitrification and Related Processes, Part B; Copyright; Contents; Contributors; Preface; Methods In Enzymology; Section One: Process Measurements in Natural Systems; Chapter 1: Strategies to Determine Diversity, Growth, and Activity of Ammonia-Oxidizing Archaea in Soil; 1. Introduction; 2. Common Methods; 3. Community Composition and Diversity; 4. Determining Growth and Abundance; 5. Activity; 6. Conclusions; References; Chapter 2: Responses of Aerobic and Anaerobic Ammonia/Ammonium-Oxidizing Microorganisms to Anthropogenic Pollution in Coastal...; 1. Introduction
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2. Selection of Sampling Sites and Physicochemical Characterization3. Molecular Ecological Characterization; 4. Community Structure Analyses; 5. Relationship Between the Community Structure and Physicochemical Parameters; 6. Relationships Between the Community Change and the Environments; 7. Conclusions; Acknowledgments; References; Chapter 3: Molecular and Stable Isotope Methods to Detect and Measure Anaerobic Ammonium Oxidation (Anammox) in Aquatic Ecosystems; 1. Introduction; 2. Molecular Methods to Detect and Quantify Anammox Bacteria in Environmental Samples
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3. Stable Isotope Methods to Measure Anammox Rates in Environmental SamplesAcknowledgments; References; Chapter 4: Nitrogen Mineralization and Assimilation at Millimeter Scales; 1. Introduction; 2. Microbial Habitats in Soil; 3. Methodological Approaches; 4. Conclusions; References; Chapter 5: Measurement of Carbon Dioxide, Methane, Nitrous oxide, and Water Potential in Soil Ecosystems; 1. Introduction; 2. Soil Gas Probes; 3. Flux Estimates to the Atmosphere Using Recirculating Chambers; 4. Data Analysis; 5. Soil Profile Analysis; 6. FTIR Calibration
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7. Correlations Between Profile Concentrations and Surface Flux in Field Measurements8. Correlations Between FTIR and Traditional Water Activity Measures; 9. Common Issues During FTIR Measurement; 10. Conclusions; Acknowledgments; References; Chapter 6: Source Determination of Nitrous Oxide Based on Nitrogen and Oxygen Isotope Tracing...; 1. Introduction; 2. Experimental Approach; 3. Data Evaluation for Nitrous Oxide Source Determination; 4. Application of the ERR Principle in Nitrate Source Determination; 5. Discussion, Applications, and Future Directions; References
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Section Two: Process Measurements in Biofilm CommunitiesChapter 7: A Polyphasic Approach to Study Ecophysiology of Complex Multispecies Nitrifying Biofilms; 1. Introduction; 2. Microsensors; 3. Microsensor Measurements; 4. Estimation of Microbial Activities; 5. Limitations of Microsensor Measurements; 6. MAR-FISH; 7. Methodology of MAR-FISH; 8. Application of Microsensors and MAR-FISH to Nitrifying Biofilms; 9. Ecophysiological Interaction Among Community Members; 10. Conclusions; References
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Chapter 8: In Situ Techniques and Digital Image Analysis Methods for Quantifying Spatial Localization Patterns of Nitrifiers...
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Electronic reproduction; Available via World Wide Web
In:
Pt. B
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Methods in enzymology Research on Nitrification and Related Processes, Part B
Sprache:
Englisch
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