UID:
almafu_9958128219102883
Umfang:
1 online resource (523 p.)
Ausgabe:
1st ed.
ISBN:
9781283704892
,
1283704897
,
9780124046177
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0124046177
Serie:
Methods in enzymology. Natural product biosynthesis by microorganisms and plants, v. 517
Inhalt:
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The third of 3 volumes covering Natural product biosynthesis by microorganisms and plants.This new volume continues the legacy of this premier serialContains quality chapters authored by leaders in the fieldThe third of 3 volumes, it has chapters on such topics as metabolic pathways in Aspergillus oryzae, heterologous gene clusters and cyanobacteria as a source of natural products
Anmerkung:
Description based upon print version of record.
,
Front Cover; Natural Product Biosynthesis by Microorganisms and Plants, Part C; Copyright; Contents; Contributors; Preface; Methods in Enzymology; Section 1: Metabolites from Novel Sources; Chapter 1: Methods for the Study of Endophytic Microorganisms from Traditional Chinese Medicine Plants; 1. Introduction; 2. Experimental Methods; 2.1. Medicinal plant selection and sampling; 2.2. Pretreatment and surface-sterilization; 2.2.1. Pretreatment; 2.2.2. Surface sterilization; 2.3. Isolation of endophytes; Media for isolation of endophytic bacteria and actinobacteria
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Media for isolation of endophytic fungi2.4. Purification and maintenance; 2.5. Taxonomic analysis; 3. Novel Bioactive Compounds from Endophytic Actinobacteria; 4. Conclusion; Acknowledgment; References; Chapter 2: Cyanobacteria as a Source of Natural Products; 1. Introduction; 2. Cyanobacteria as Starting Material for Natural Products; 2.1. Isolation of cyanobacterial strains; 2.2. Mass cultivation of cyanobacterial strains; 2.2.1. Protocol for mass cultivation of cyanobacteria in a laboratory scale; 2.3. Harvesting cyanobacteria from natural habitats
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2.3.1. Protocol: Harvesting cyanobacterial cells from field habitats3. Screening for New Compounds and Producers; 3.1. Screening for new compounds; 3.1.1. Protocol: Screening by whole-cell mass spectrometry of freeze-dried cyanobacterial biomass for the presence of mic ...; 3.2. Characterization of (new?) natural products; 3.3. Finding new sources of known compounds; 4. Genomic Mining of Cyanobacterial Natural Products; 4.1. Mutagenesis of biosynthetic genes in cyanobacteria; 4.1.1. Genetic manipulation of cyanobacteria; 4.2. Transcriptional activation of biosynthetic genes in cyanobacteria
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4.3. Heterologous production of natural products from cyanobacteria4.4. In vitro reconstitution of cyanobacterial biosynthetic pathways; 5. Summary; References; Chapter 3: Isolating Antifungals from Fungus-Growing Ant Symbionts Using a Genome-Guided Chemistry Approach; 1. Introduction; 2. Strain Isolation and Genome Sequencing; 2.1. Selectively isolating actinomycetes; 2.2. Bioassaying for antifungal activity; 2.3. Genome sequencing and analysis of Pseudonocardia P1 and S. albus S4; 3. Identifying Antifungals made by Pseudonocardia P1; 3.1. Searching for antifungal pathways in the P1 genome
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3.2. Chemical identity of nystatin P13.3. Attempts at heterologous expression; 4. Identifying Antifungals made by S. albus S4; 4.1. Searching for antifungal pathways in the S4 genome; 4.2. Disrupting candicidin biosynthesis; 4.3. Identification of the antimycin gene cluster; 4.4. Cloning the antimycin gene cluster; 4.5. Other gene clusters in the S4 genome; 5. Summary and Perspectives; Acknowledgments; References; Chapter 4: Gamma-Butyrolactone and Furan Signaling Systems in Streptomyces; 1. Introduction; 2. AfsA-Like Enzymes and the Biosynthesis of Signaling Molecules
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2.1. A-factor biosynthesis by AfsA
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English
Weitere Ausg.:
ISBN 9780124046344
Weitere Ausg.:
ISBN 0124046347
Sprache:
Englisch
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