UID:
almafu_9958128035202883
Umfang:
1 online resource (565 p.)
ISBN:
9780124166189
,
0124166180
,
9780124166578
,
0124166571
Serie:
Methods in Enzymology,
Inhalt:
Volume 542 of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This new volume covers research methods providing a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to study oncometabolism, in vitro, ex vivo and in vivo. Malignant cells exhibit metabolic changes when compared to their normal counterparts, owing to both genetic and epigenetic alterations. Although such a metabolic rewiring has recently been indicated as ""yet another"" general hallmark of ca
Anmerkung:
Description based upon print version of record.
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Front Cover; Conceptual Background and Bioenergetic/Mitochondrial Aspects of Oncometabolism; Copyright; Contents; Contributors; Preface: Oncometabolism: A New Field of research With Profound Therapeutic Implications; Acknowledgments; References; Chapter One: The Metabolic Alterations of Cancer Cells; 1. Introduction; 2. Aerobic Glycolysis: A Hallmark of Cancer Cell Metabolism; 2.1. Aerobic glycolysis and the energy requirements of proliferation; 2.2. The branching of glycolysis is a tightly controlled process
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2.2.1. Phosphofructokinase 1 and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatases2.2.2. Pyruvate kinase; 2.2.3. 3-Phosphoglycerate dehydrogenase; 2.2.4. Phosphoglycerate mutase; 2.2.5. 1,3-Bisphosphoglycerate regulates glycolytic flux; 3. Mitochondrial Metabolism in Cancer Cells; 3.1. Mitochondria and the anabolic requirements of cancer cells; 3.2. Mitochondrial tumor suppressors; 3.3. Isocitrate dehydrogenase (IDH): A bona fide mitochondrial (and cytosolic) oncogene; 4. Lipid Metabolism in Cancer Cells; 4.1. Fatty acid biosynthesis supports cancer cell proliferation
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5. Future Perspectives: Altered Metabolism Shapes Tumor Evolution6. Conclusions; References; Chapter Two: Autophagy and Cancer Metabolism; 1. Introduction; 2. Overview of the Autophagy Machinery; 2.1. Initiation and the ULK complex; 2.2. Nucleation and Beclin 1/ATG14/VPS34 complex; 2.3. Elongation and the ATG12/ATG8 conjugation systems; 2.4. Fusion; 2.5. Chaperone-mediated autophagy; 3. Metabolic Stimuli Regulating Autophagy; 3.1. Nutrient starvation; 3.2. Glucose; 3.3. Amino acids; 3.4. Glutamine; 3.5. Lipids and free fatty acids; 3.6. Hypoxia and ROS; 4. Autophagy and Tumor Suppression
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4.1. ATGs as tumor suppressors4.2. Autophagy-dependent degradation of p62/SQSTM1; 4.3. Autophagy prevents protumor inflammation and facilitates senescence; 4.4. Autophagy clears dysfunctional mitochondria; 5. Tumor-Promoting Functions of Autophagy; 5.1. Autophagy and metabolic adaptation in cancer; 5.1.1. Autophagy and oxidative mitochondrial metabolism; 5.1.2. Glucose metabolism; 5.1.3. Amino acids; 5.1.4. Lipids; 5.2. Autophagy promotes cell survival under metabolic stress; 5.3. Autophagy in the tumor stroma; 5.4. Autophagy inhibition in cancer therapy; 6. Conclusion; Acknowledgments
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ReferencesChapter Three: Regulation of Cancer Metabolism by Oncogenes and Tumor Suppressors; 1. Introduction; 2. HIF-1: Regulator of Hypoxic Responses and Cancer Metabolism; 3. The PI3K-AKT-PTEN Pathway Regulates Metabolism; 4. mTOR Controls Anabolism and It Is Inhibited By AMPK Upon Metabolic Stress; 5. c-Myc Promotes Aerobic Anabolism; 6. Ras Stimulates Glycolysis and the PPP; 7. NF-kappaB Regulates Inflammation and Proliferation But Also Metabolism; 8. Retinoblastoma: Suppressing Tumorogenesis and Anabolism; 9. p53 Regulates Multiple Metabolic Pathways; 10. Conclusions; Acknowledgments
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References
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English
Weitere Ausg.:
ISBN 9781306820646
Weitere Ausg.:
ISBN 1306820642
Sprache:
Englisch
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