UID:
almafu_9958130098102883
Umfang:
1 online resource (xxii, 332 pages, 20 unnumbered pages of plates) :
,
illustrations (some color).
ISBN:
9780124167469
,
0124167462
Serie:
Methods in Cell Biology, Volume 119
Inhalt:
This new volume of Methods in Cell Biology looks at micropatterning in cell biology and includes chapters on protein photo-patterning on PEG with benzophenone, laser-directed cell printing and dip pen nanolithography. The cutting-edge material in this comprehensive collection is intended to guide researchers for years to come. Includes sections on micropatterning in 2D with photomask, maskless micropatterning and 2D nanopatterningChapters are written by experts in the fieldCutting-edge material
Anmerkung:
"ISSN: 0091-679X."
,
Front Cover; Micropatterning in Cell Biology Part A; Copyright; Contents; Contributors; Preface; Biography; Section 1: Micro-Patterning in 2D; Chapter 1: "Stamp-off" to Micropattern Sparse, Multicomponent Features; Introduction; 1.1. Method; 1.2. Discussion; Acknowledgments; References; Chapter 2: Poly(Vinyl Alcohol)- Micropatterned Surfaces for Manipulation of Mesenchymal Stem Cell Functions; Introduction; 2.1. Preparation of PVA-Micropatterned Surfaces; 2.1.1. Synthesis of photo-reactive PVA; 2.1.2. Micropatterning of PVA
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2.2. Effect of Cell Density and Cell-Cell Interaction on Adipogenic, Chondrogenic, and Osteogenic Differentiation of MSCs2.3. Effect of Cell Spreading on Adipogenic and Osteogenic Differentiation of MSCs; 2.4. Effect of Cell Geometry on Adipogenic Differentiation of MSCs; 2.5. Effect of Cell Protrusion on Adipogenic Differentiation of MSCs; 2.6. Effect of Surface Charge on Adipogenic Differentiation of MSCs; Summary; Acknowledgments; References; Chapter 3: Patterning of Polymeric Cell Culture Substrates; Introduction; Patterning of Self-Assembled Monolayers; Polymer patterning
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3.1. Equipment and Protocols3.1.1. Safety and general recommendations; 3.1.2. The UV lamp; 3.1.3. UV dosimetry; 3.1.4. Lithography masks; 3.1.5. Substrates; 3.1.5.1. Protocol for spin coating of Si wafers or glass with PS films; 3.1.6. UV irradiation; 3.1.7. Cell culture and media supplements; 3.1.8. Advanced technology; 3.1.8.1. Protocol; 3.1.9. Problems and workarounds; 3.1.9.1. Proximity gap; 3.1.9.2. Inhomogeneous seeding; 3.2. Results and Discussion; 3.2.1. Surface characterization; 3.2.2. Cell adhesion; 3.2.3. Competitive protein adsorption; Conclusions; Acknowledgments; References
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Chapter 4: Photopatterning with a Printed Transparency Mask and a Protein-Friendly PhotoresistIntroduction; 4.1. Synthesis and Characterization of a Protein-Friendly Photoresist PDMP; 4.1.1. Materials; 4.1.2. Equipment; 4.1.3. Method; 4.1.4. Key tips for the synthesis of PDMP with desired properties; 4.2. Microscope Projection Photolithography; 4.2.1. Materials; 4.2.2. Equipment; 4.2.3. Method; 4.3. Micropatterning of Multiple Lymphocytes; 4.3.1. Materials; 4.3.2. Equipment; 4.3.3. Method; 4.4. Dynamic Micropatterning of Adhering Cells; 4.4.1. Materials; 4.4.2. Equipment; 4.4.3. Method
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Summary and OutlookAcknowledgments; References; Chapter 5: Plasma Microcontact Patterning (PmCP): A Technique for the Precise Control of Surface Patterning at Small-Scale; Introduction; 5.1. Methods; 5.1.1. 3D mask design and fabrication; 5.1.2. Master fabrication; 5.1.2.1. Surface precoating; 5.1.2.2. Plasma micropatterning (step 2); 5.1.2.3. Surface postcoating (step 3); 5.2. Cell Patterning; 5.3. Results; 5.3.1. Precise control of the surface patterning; 5.3.2. PμCP-inverted techniques: comparison with the standard PμCP; 5.4. Discussion; Acknowledgments; References
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Chapter 6: Patterning Gradients
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English
Weitere Ausg.:
ISBN 9780124167421
Weitere Ausg.:
ISBN 012416742X
Weitere Ausg.:
ISBN 9781306370004
Weitere Ausg.:
ISBN 1306370000
Sprache:
Englisch
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