Kooperativer Bibliotheksverbund

UID:

Umfang: 1 online resource (535 p.)

Ausgabe: First edition.

ISBN: 0-12-417175-3

Serie: Methods in enzymology, volume 544

Inhalt: Regulated Cell Death Part A & Part B of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in apoptosis focusing on the important areas of intrinsic pathway, extrinsic pathway, caspases, cellular assays and post-apoptotic effects and model organisms; as well as topics on necroptosis and screening approaches.Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science〈li

Anmerkung: Description based upon print version of record. , Front Cover; Regulated Cell Death Part A: Apoptotic Mechanisms; Copyright; Contents; Contributors; Preface; Chapter One: Examining the Molecular Mechanism of Bcl-2 Family Proteins at Membranes by Fluorescence Spectroscopy; 1. Introduction; 2. An In Vitro Fluorescence-Based Liposome System; 2.1. Expression and purification of Bcl-2 family proteins; 2.1.1. Expression of Bax, Bcl-XL, and Bid; 2.1.2. Purification of Bax and Bcl-XL; 2.1.3. Purification of cBid; 2.1.4. Buffer recipes; 2.1.4.1. Bax; 2.1.4.2. Bcl-XL; 2.1.4.3. Bid; 2.1.4.4. Dialysis; 2.2. Site-specific protein labeling , 2.3. Production of mitochondria-like liposomes2.3.1. Preparing lipid films and generating liposomes; 3. Membrane Permeabilization Assay; 3.1. ANTS/DPX release assay; 4. Fluorescence Resonance Energy Transfer; 4.1. Detecting the interaction between two proteins using FRET; 5. Tracking the Conformation Changes of a Protein; 5.1. NBD-emission assay; 6. Determining the Topology of Proteins within Membranes; 6.1. Iodide quenching of NBD-labeled Bax; 7. Conclusion; Acknowledgments; References , Chapter Two: Photoreactive Stapled Peptides to Identify and Characterize BCL-2 Family Interaction Sites by Mass Spectrometry1. Introduction; 2. Overview of Photoreactive Stabilized Alpha-Helices Methodology; 3. Design and Synthesis of Photoreactive Stapled Peptides; 4. Photoaffinity Labeling and Retrieval; 5. Mass Spectrometry Analysis; 6. Computational Docking; 7. Mapping Novel Binding Sites; 8. Conclusions and Future Directions; Acknowledgments; References; Chapter Three: The Structural Biology of BH3-Only Proteins; 1. Introduction; 2. What Constitutes a BH3-Only Protein? , 3. Specificity and Interactions with the Bcl-2 Family4. Structures of BH3-Only Proteins and Their Complexes; 5. Interaction of BH3-Only Proteins with Viral Bcl-2 Homologues; 6. Interaction with Non-Bcl-2 Proteins: Beyond the BH3-Motif; 7. Regulation; 8. BH3-Only Proteins and Disease; 9. Conclusion; Acknowledgments; References; Chapter Four: How to Analyze Mitochondrial Morphology in Healthy Cells and Apoptotic Cells in Caenorhabditis elegans; 1. Introduction; 2. Why Address These Questions in C. elegans; 3. How to Inactivate or Overexpress a Particular Candidate Gene in C. elegans , 3.1. Inactivation of a particular candidate gene3.1.1. Loss-of-function mutants; 3.1.2. Inactivation by RNA interference; 3.2. Overexpression of a particular candidate gene; 3.2.1. In embryos, using heat-shock promoters; 3.2.2. In specific cell types, using smg-inducible system; 4. How to Visualize Mitochondria in C. elegans; 4.1. General considerations for imaging; 4.2. Mitochondrial membrane potential-sensitive dyes; 4.2.1. TMRE, Mitotracker, and others dyes; 4.2.2. Protocol to visualize mitochondria in embryos using TMRE; 4.2.2.1. Media and solutions; 4.2.2.2. Materials; 4.2.2.3. Protocol , 4.2.2.4. Specific protocol for TMRE staining of animals treated with RNAi , English

Weitere Ausg.: ISBN 0-12-417158-3

Weitere Ausg.: ISBN 1-306-90247-9

Sprache: Englisch

UID:

Umfang: 1 Online-Ressource

Ausgabe: 1. ed.

ISBN: 9780124171589 , 9780124171756

Serie: Methods in enzymology 544

In: 1

Sprache: Englisch

Fachgebiete: Biologie

URL: Volltext  (URL des Erstveröffentlichers)

UID:

Umfang: Online-Ressource (XV, 482 S.) , Ill., graph. Darst.

Ausgabe: Online-Ausg.

ISBN: 9780124171589 , 0124171753 , 9780124171756

Serie: Regulated cell death / ed. by Yunying Yuan... Pt. A

Anmerkung: Includes bibliographical references and index , Part A. Apoptotic mechanism. Examining the molecular mechanism of Bcl-2 family proteins at membranes by fluorescence spectroscopy / Justin Kale, Xiaoke Chi, Brian Leber, and David Andrews ; Photoreactive stapled peptides to identify and characterize BCL-2 family interaction sites by mass spectrometry / Susan Lee, Craig R. Braun, Gregory H. Bird, and Loren D. Walensky ; The structural biology of BH3-only proteins / Marc Kvansakul and Mark G. Hinds ; How to analyze mitochondrial morphology in healthy cells and apoptotic cells in Caenorhabditis elegans / Stéphane G. Rolland ; Apoptosis initiation through the cell-extrinsic pathway / Pradeep Nair, Min Lu, Sean Petersen, and Avi Ashkenazi ; Using RNAi screening technologies to interrogate the extrinsic apoptosis pathway / Jean Philippe Stephan ; Caspase enzymology and activation mechanisms / Peter D. Mace, Stefan J. Riedl, and Guy S. Salvesen ; Turning ON caspases with genetics and small molecules / Charles W. Morgan, Olivier Julien, Elizabeth K. Unger, Nirao M. Shah, and James A. Wells ; A multipronged approach for compiling a global map of allosteric regulation in the apoptotic caspases / Kevin Dagbay, Scott J. Eron, Banyuhay P. Serrano, Elih M. Velázquez-Delgado, Yunlong Zhao, Di Lin, Sravanti Vaidya, and Jeanne A. Hardy ; Measuring caspase activity in vivo / Samantha B. Nicholls and Bradley T. Hyman ; Single-molecule sensing of caspase activation in live cells via plasmon coupling nanotechnology / Cheryl Tajon, Young-Wook Jun, and Charles S. Craik ; In vivo monitoring of caspase activation using a fluorescence resonance energy transfer-based fluorescent probe / Yoshifumi Yamaguchi, Erina Kuranaga, Yu-ichiro Nakajima, Akiko Koto, Kiwamu Takemoto, and Masayuki Miura ; Global analysis of cellular proteolysis by selective enzymatic labeling of protein N-termini / Arun P. Wiita, Julia E. Seaman, and James A. Wells ; Complementary methods for the identification of substrates of proteolysis / Victoria C. Pham, Veronica G. Anania, Qui T. Phung, and Jennie R. Lill ; Phospholipid scrambling on the plasma membrane / Jun Suzuki and Shigekazu Nagata ; Studying apoptosis in the zebrafish / Peter M. Eimon

Sprache: Englisch

Schlagwort(e): Apoptosis ; Aufsatzsammlung

URL: Volltext

URL: Volltext

UID:

Umfang: 1 online resource (535 p.)

Ausgabe: First edition.

ISBN: 0-12-417175-3

Serie: Methods in enzymology, volume 544

Inhalt: Regulated Cell Death Part A & Part B of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in apoptosis focusing on the important areas of intrinsic pathway, extrinsic pathway, caspases, cellular assays and post-apoptotic effects and model organisms; as well as topics on necroptosis and screening approaches.Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science〈li

Anmerkung: Description based upon print version of record. , Front Cover; Regulated Cell Death Part A: Apoptotic Mechanisms; Copyright; Contents; Contributors; Preface; Chapter One: Examining the Molecular Mechanism of Bcl-2 Family Proteins at Membranes by Fluorescence Spectroscopy; 1. Introduction; 2. An In Vitro Fluorescence-Based Liposome System; 2.1. Expression and purification of Bcl-2 family proteins; 2.1.1. Expression of Bax, Bcl-XL, and Bid; 2.1.2. Purification of Bax and Bcl-XL; 2.1.3. Purification of cBid; 2.1.4. Buffer recipes; 2.1.4.1. Bax; 2.1.4.2. Bcl-XL; 2.1.4.3. Bid; 2.1.4.4. Dialysis; 2.2. Site-specific protein labeling , 2.3. Production of mitochondria-like liposomes2.3.1. Preparing lipid films and generating liposomes; 3. Membrane Permeabilization Assay; 3.1. ANTS/DPX release assay; 4. Fluorescence Resonance Energy Transfer; 4.1. Detecting the interaction between two proteins using FRET; 5. Tracking the Conformation Changes of a Protein; 5.1. NBD-emission assay; 6. Determining the Topology of Proteins within Membranes; 6.1. Iodide quenching of NBD-labeled Bax; 7. Conclusion; Acknowledgments; References , Chapter Two: Photoreactive Stapled Peptides to Identify and Characterize BCL-2 Family Interaction Sites by Mass Spectrometry1. Introduction; 2. Overview of Photoreactive Stabilized Alpha-Helices Methodology; 3. Design and Synthesis of Photoreactive Stapled Peptides; 4. Photoaffinity Labeling and Retrieval; 5. Mass Spectrometry Analysis; 6. Computational Docking; 7. Mapping Novel Binding Sites; 8. Conclusions and Future Directions; Acknowledgments; References; Chapter Three: The Structural Biology of BH3-Only Proteins; 1. Introduction; 2. What Constitutes a BH3-Only Protein? , 3. Specificity and Interactions with the Bcl-2 Family4. Structures of BH3-Only Proteins and Their Complexes; 5. Interaction of BH3-Only Proteins with Viral Bcl-2 Homologues; 6. Interaction with Non-Bcl-2 Proteins: Beyond the BH3-Motif; 7. Regulation; 8. BH3-Only Proteins and Disease; 9. Conclusion; Acknowledgments; References; Chapter Four: How to Analyze Mitochondrial Morphology in Healthy Cells and Apoptotic Cells in Caenorhabditis elegans; 1. Introduction; 2. Why Address These Questions in C. elegans; 3. How to Inactivate or Overexpress a Particular Candidate Gene in C. elegans , 3.1. Inactivation of a particular candidate gene3.1.1. Loss-of-function mutants; 3.1.2. Inactivation by RNA interference; 3.2. Overexpression of a particular candidate gene; 3.2.1. In embryos, using heat-shock promoters; 3.2.2. In specific cell types, using smg-inducible system; 4. How to Visualize Mitochondria in C. elegans; 4.1. General considerations for imaging; 4.2. Mitochondrial membrane potential-sensitive dyes; 4.2.1. TMRE, Mitotracker, and others dyes; 4.2.2. Protocol to visualize mitochondria in embryos using TMRE; 4.2.2.1. Media and solutions; 4.2.2.2. Materials; 4.2.2.3. Protocol , 4.2.2.4. Specific protocol for TMRE staining of animals treated with RNAi , English

Weitere Ausg.: ISBN 0-12-417158-3

Weitere Ausg.: ISBN 1-306-90247-9

Sprache: Englisch