UID:
almafu_9958130294202883
Format:
1 online resource (lix, 347 pages) :
,
illustrations (some color).
Edition:
First edition.
ISBN:
9780124200944
,
012420094X
Series Statement:
Methods in enzymology ; volume five hundred and thirty three
Content:
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsIn
Note:
"ISSN: 0076-6879."
,
Front Cover; Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate; Copyright; Contents; Contributors; Miscellaneous; Preface; Methods in Enzymology; Section I: Cell Protocols: Basic Microbiological Techniques; Chapter One: Pouring Agar Plates and Streaking or Spreading to Isolate Individual Colonies; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Preparation of LB-Agar Solution; 5.1. Overview; 5.2. Duration; 5.3. Caution; 5.4. Tip; 5.5. Tip; 5.6. Tip; 5.7. Tip; 6. Step 2 Pouring Agar Plates; 6.1. Overview
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6.2. Duration6.3. Tip; 6.4. Caution; 6.5. Tip; 6.6. Tip; 7. Step 3 Streaking for Individual Colonies; 7.1. Overview; 7.2. Duration; 7.3. Tip; 7.4. Tip; 7.5. Caution; 8. Step 4 Spreading Cells on Agar Plates; 8.1. Overview; 8.2. Duration; 8.3. Tip; 8.4. Tip; 8.5. Caution; References; Referenced Protocols in Methods Navigator; Chapter Two: Storage of Bacteria and Yeast; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 5. Protocol A Cryopreservation of Bacterial Cultures; 5.1. Preparation; 5.2. Duration; 6. Step 1A Grow Bacterial Cultures; 6.1. Overview
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6.2. Duration7. Step 2A Freeze the Bacterial Cells; 7.1. Overview; 7.2. Duration; 8. Protocol B Cryopreservation of Yeast; 8.1. Preparation; 8.2. Duration; 9. Step 1B Grow Yeast Cultures; 9.1. Overview; 9.2. Duration; 10. Step 2B Freeze the Yeast Cells; 10.1. Overview; 10.2. Duration; References; Referenced Protocols in Methods Navigator; Section II: Cell Protocols: Cellular Fractionation; Chapter Three: Cellular Fractionation - Mammalian Cells; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation
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5. Step 1 Subcellular Mammalian Cell Fractionation5.1. Overview; 5.2. Duration; 5.3. Tip; Source References; Related Literature; Chapter Four: Cellular Fractionation - Yeast Cells; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Yeast Protoplast Formation; 5.1. Overview; 5.2. Duration; 5.3. Tip; 6. Step 2 Subcellular Fractionation of Yeast Protoplasts; 6.1. Overview; 6.2. Duration; 6.3. Tip; Source References; Related Literature; Section III: Cell Protocols: Knock-outs and Gene Replacements
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Chapter Five: Yeast-Gene Replacement Using PCR Products1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Generation of a Linear Integrating Construct by PCR; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 6. Step 2 Visualization of Product on an Agarose Gel; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Caution; 7. Step 3 Transforming Yeast with a Linear PCR Product for Integration; 7.1. Overview; 7.2. Duration; 7.3. Tip; 8. Step 4 Verifying Appropriate Integration in Colonies Growing on Selective Media
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8.1. Overview
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English
Additional Edition:
ISBN 9780124200678
Additional Edition:
ISBN 0124200672
Additional Edition:
ISBN 9781306087285
Additional Edition:
ISBN 1306087287
Language:
English
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