UID:
almafu_9958130098202883
Umfang:
1 online resource (xv, 180 pages) :
,
illustrations (some color).
ISBN:
9780124200975
,
0124200974
Serie:
Methods in enzymology, Volume 536
Inhalt:
In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols. In this volume we have brought together a number of core protocols concentrating on Protein.
Anmerkung:
"ISSN: 0076-6879."
,
Front Cover; Laboratory Methods in Enzymology: Protein Part A; Copyright; Contents; Contributors; Preface; Section I: Protein Protocols; Chapter One: Practical Steady-State Enzyme Kinetics; 1. Theory; 2. Equipment; 3. Materials; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Measure Initial Rates of the Enzyme-Catalyzed Reaction as a Function of Substrate Concentration; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 5.6. Tip; 5.7. Tip; 6. Step 2 Determine the Kinetic Parameters (Vmax, kcat, Km); 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Tip; 6.6. Tip
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6.7. Tip7. Step 3 Analyze the Mode of Action of an Inhibitor; 7.1. Overview; 7.2. Duration; 7.3. Tip; 7.4. Tip; References; Referenced Literature; Referenced Protocols in Methods Navigator; Chapter Two: Quantification of Protein Concentration Using UV Absorbance and Coomassie Dyes; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol 1; 4.1. Duration; 4.2. Preparation; 5. Step 1 Quantification of Protein Using UV Absorbance; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 5.6. Tip; 5.7. Tip; 6. Protocol 2; 6.1. Duration; 6.2. Preparation
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7. Step 1 Quantification of Protein Using the Coomassie (Bradford) Assay7.1. Duration; 7.2. Preparation; 7.3. Caution; 7.4. Overview; 7.5. Duration; 7.6. Tip; 7.7. Tip; 7.8. Tip; 7.9. Tip; 7.10. Tip; 7.11. Tip; References; Referenced Literature; Source References; Referenced Protocols in Methods Navigator; Chapter Three: Preparation of Protein Samples for Mass Spectrometry and N-Terminal Sequencing; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Method A Preparation of Protein Samples for Mass Spectrometry; 4.1. Duration; 4.2. Preparation
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5. Step A1 Purify the Mitochondria by Metrizamide Gradient Centrifugation and Solubilize Them5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 6. Step A2 Fractionate the Solubilized Mitochondria by Sucrose Density Gradient Sedimentation; 6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step A3 Separate the Proteins by SDS-PAGE; 7.1. Overview; 7.2. Duration; 7.3. Tip; 7.4. Tip; 8. Method B Preparation of Protein Samples for N-Terminal Sequencing; 8.1. Duration; 8.2. Preparation; 9. Step B1 Prepare Whole Cell Lysates of the Cells; 9.1. Overview; 9.2. Duration
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10. Step B2 Affinity Purify the Protein of Interest10.1. Overview; 10.2. Duration; 10.3. Tip; 11. Step B3 Separate Proteins by SDS-PAGE and Transfer to PVDF Membrane; 11.1. Overview; 11.2. Duration; 12. Step B4 Stain the PVDF Membrane and Take It to Your Protein Sequencing Facility; 12.1. Overview; 12.2. Duration; 12.3. Tip; 12.4. Tip; References; Referenced Literature; Referenced Protocols in Methods Navigator; Section II: Protein Protocols/Cell Lysis; Chapter Four: Lysis of Mammalian and Sf9 Cells; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration
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4.2. Preparation
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English
Weitere Ausg.:
ISBN 9780124200708
Weitere Ausg.:
ISBN 0124200702
Weitere Ausg.:
ISBN 9781306321747
Weitere Ausg.:
ISBN 1306321743
Sprache:
Englisch
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