UID:
edoccha_9960073786602883
Format:
1 online resource (137 p.)
Edition:
1st ed.
ISBN:
0-12-800719-2
Note:
Description based upon print version of record.
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Front Cover -- Practical NMR Spectroscopy Laboratory Guide: Using Bruker Spectrometers -- Copyright Page -- Contents -- Acknowledgments -- Introduction -- Overview -- NMR Samples Referenced in the Text -- Spectrometer Requirements -- Activities -- Reference -- 1 Basics and Spectrometer Performance Checks -- Overview -- Sample and Spectrometer Requirements -- Activities -- Part 1-Familiarization -- Part 2-Shimming and Lineshape -- Part 3-Pulse Calibration and Sensitivity for 1H -- Part 4-Pulse Calibration and Sensitivity for 13C -- Part 5-Viewing and Editing Text Files in TopSpin -- Summary -- Appendix 1.1 Standard Parameter Sets -- Appendix 1.2 Probe Tuning -- Tuning the 1H Coil-All Probes -- Spectrometer Setup-X-Nucleus Tuning -- Probe Tuning-Using ATMM or ATMA Routine -- Appendix 1.3 Quinine Spectra -- Appendix 1.4 Lineshape Effects of Nonspinning Shims -- Appendix 1.5 Pulse Width Calibration with paropt -- 2 Multiple Irradiation and Multiple Pulse Experiments -- Overview -- Sample and Spectrometer Requirements -- Activities -- Part 1-1H T1 (Spin-Lattice) Relaxation Measurement -- Part 2-Homonuclear Decoupling -- Part 3-nOe-difference -- Part 4-1H-decoupled 13C Spectra with and without nOe -- Part 5-13C DEPT Spectra -- Summary -- 3 Polarization Transfer and Its Applications -- Overview -- Sample and Spectrometer Requirements -- Activities -- Part 1-Polarization Transfer via Selective Irradiation -- Part 2-Polarization Transfer via INEPT -- Part 3-Heteronuclear Correlation Experiment Setup and Acquisition -- Part 4-Heteronuclear Correlation Experiment Data Processing -- Part 5-Heteronuclear Correlation Experiments on Quinine-Experiment Setup -- Part 6-Heteronuclear Correlation Experiments on Quinine-Data Processing -- Summary -- 4 Homonuclear Correlation Experiments -- Overview -- Sample and Spectrometer Requirements -- Activities.
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Part 1-Quinine 1H Spectrum -- Part 2-COSY Setup -- Part 3-TOCSY Setup -- Part 4-NOESY Setup -- Part 5-2D Data Acquisition -- Part 6-COSY Data processing -- Part 7-TOCSY Data Processing -- Part 8-NOESY Data Processing -- Summary -- Appendix 4.1 Using edprosol to Determine the Power Level for the TOCSY Spinlock Pulse P6 -- 5 PFG (Pulsed Field Gradient) Experiments -- Overview -- Sample and Spectrometer Requirements -- Activities -- Part 1-1D Z-Gradient Image of an H2O/D2O "Phantom" -- Part 2-pfg-Homonuclear 2D Experiments Setup -- Part 3-2D pfg-Homonuclear Experiments Data Acquisition -- Part 4-pfg-Homonuclear 2D Experiments Data Processing -- Part 5-pfg-Heteronuclear 2D Experiments Setup -- Part 6-2D Data Acquisition -- Part 7-pfg-Heteronuclear 2D Experiments Data Processing -- Summary -- Appendix 5.1 Regarding the "Phantom" Sample -- 6 Introduction to NMR of Biomolecules in H2O -- Overview -- Sample and Spectrometer Requirements -- Activities -- Part 1-Lysozyme Spectra-Shimming and Pulse Calibration -- Part 2-Lysozyme Spectra-Water Suppression Using Presaturation-Based Methods -- Part 3-Lysozyme Spectra-Water Suppression Using "WATERGATE"-Based Methods -- Part 4-Lysozyme Spectra-2D NOESY with WATERGATE Water Suppression -- Part 5-Ubiquitin Spectra-Shimming, Pulse Calibration and 1D 1H Spectrum -- Part 6-Ubiquitin 2D 13C HSQC (Heteronuclear Single-Quantum) Experiment Setup and Acquisition -- Part 7-Ubiquitin 2D 15N HSQC Experiment Setup and Acquisition -- Part 8-Ubiquitin 2D HSQC Experiments Data Processing -- Summary -- Appendix 6.1 Mapping Shim Profiles in Gradshim -- Appendix 6.2 Presat180 Pulse Sequence -- Appendix 6.3 X-Nucleus Pulse Calibration Using the gs-mode -- Appendix 6.4 Routing for the 15N RF Channel in the 15N HSQC Experiment -- 7 Selective Experiments Using Shaped Pulses -- Overview -- Sample and Spectrometer Requirements -- Activities.
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Part 1-1H Setup -- Part 2-Selective Pulse Calculation and Simulation -- Part 3-Selective Pulse Calibration -- Part 4-Selective TOCSY with 270° Selective Pulse -- Part 5-Selective TOCSY with 180° Selective Pulse and Field Gradients -- Part 6-Selective NOESY with 270° Selective Pulse -- Part 7-Selective NOESY with 180° Selective Pulse and Field Gradients -- Part 8-Quantitation by NMR (qNMR)-Introduction -- Part 9a-Quantitation by NMR (qNMR)-Sample I -- Part 9b-Quantitation by NMR (qNMR)-Sample J -- Summary -- Appendix 7.1 Quantitation Samples I and J Preparation -- 8 Diffusion Measurements and DOSY (Diffusion Ordered SpectroscopY) -- Overview -- Sample and Spectrometer Requirements -- Activities -- Part 1-VT Calibration -- Part 2-Diffusion of H2O in D2O -- Part 3-DOSY Spectrum of Sample M -- Summary -- Back Cover.
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English
Additional Edition:
ISBN 0-12-800689-7
Language:
English
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