UID:
edoccha_9958079899702883
Format:
1 online resource (516 p.)
Series Statement:
Translational Epigenetics Series
Content:
Epigenetic Technological Applications is a compilation of state-of-the-art technologies involved in epigenetic research. Epigenetics is an exciting new field of biology research, and many technologies are invented and developed specifically for epigenetics study. With chapters covering the latest developments in crystallography, computational modeling, the uses of histones, and more, Epigenetic Technological Applications addresses the question of how these new ideas, procedures, and innovations can be applied to current epigenetics research, and how they can keep pushing discovery forward and
Note:
Description based upon print version of record.
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Front Cover; Epigenetic Technological Applications; Copyright Page; Contents; List of Contributors; Foreword; 1 The State of the Art of Epigenetic Technologies; 1.1 Epigenetics and Chromatin Function; 1.2 Mechanisms of Epigenetic Regulation; 1.3 Technologies are Critical for the Advancement of Epigenetic Discovery; 1.4 Epigenetic Inhibitors as Novel Therapeutics; 1.5 Perspective; Acknowledgments; References; 2 Technologies for the Measurement and Mapping of Genomic 5-Methylcytosine and 5-Hydroxymethylcytosine; 2.1 Introduction
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2.2 Measuring Genomic Levels of 5-Methylcytosine and 5-Hydroxymethylcytosine2.3 Locus-Specific Analysis of 5-Methylcytosine and 5-Hydroxymethylcyotsine; 2.4 Technologies for Genome-Wide Analysis of 5-Methylcytosine and 5-Hydroxymethylcytosine; 2.4.1 DNA Microarray Approaches; 2.4.2 Next-Generation Sequencing Approaches; 2.5 Conclusions; Acknowledgments; References; 3 High-Throughput Sequencing-Based Mapping of Cytosine Modifications; 3.1 Introduction; 3.2 Cytosine Modifications; 3.2.1 5-Methylcytosine; 3.2.2 5-Hydroxymethylcytosine; 3.2.3 5-Formylcytosine and 5-Carboxylcytosine
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3.3 High-Throughput Sequencing Methods for Detecting Cytosine Modifications3.3.1 Genome-Wide Mapping of Cytosine Modifications; 3.3.1.1 DNA immunoprecipitation; 3.3.1.2 Protein-based capture; 3.3.1.2.1 MBD capture; 3.3.1.2.2 JBP1 capture; 3.3.1.3 Chemical-labeling-based capture; 3.3.1.3.1 hMe-seal; 3.3.1.3.2 Oxyamine-based 5fC enrichment; 3.3.1.3.3 fC-seal; 3.3.2 Single-Base Resolution Methods of Detecting Cytosine Modifications; 3.3.2.1 BS-Seq for 5mC; 3.3.2.2 TAB-Seq for 5hmC; 3.3.2.3 oxBS-Seq for 5hmC; 3.3.2.4 Aba-Seq for 5hmC; 3.3.2.5 fCAB-Seq for 5fC; 3.3.2.6 redBS-Seq for 5fC
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3.3.2.7 CAB-Seq for 5caC3.4 Conclusions; References; 4 Application of Mass Spectrometry in Translational Epigenetics; 4.1 Introduction; 4.2 Applications of Mass Spectrometry in Epigenetic Research; 4.2.1 Principles of Mass Spectrometry in Proteomics; 4.2.2 Major Approaches for Mass-Spectrometry-Based Histone PTM Analysis; 4.2.2.1 Sample preparation for histone analysis; 4.2.2.1.1 Histone isolation; 4.2.2.1.2 Histone isotype separation; 4.2.2.1.3 PTM enrichment; 4.2.2.2 Mass spectrometry approaches for histone PTM analysis; 4.2.2.2.1 Bottom-up; 4.2.2.2.2 Top-down; 4.2.2.2.3 Middle-down
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4.2.3 Bioinformatics for Histone Code Analysis4.2.4 Quantification and Dynamics of Histone PTMs by Mass Spectrometry; 4.2.4.1 Label-Free Method; 4.2.4.2 Stable isotopic labeling method; 4.2.4.2.1 Metabolic labeling; 4.2.4.2.2 Chemical tags; 4.2.4.2.3 Internal standards spike-in; 4.3 Conclusion; References; 5 Techniques Analyzing Chromatin Modifications at Specific Single Loci; 5.1 Gene Expression Pattern is Related to the Covalent Modification of Core Histones; 5.2 Heterochromatin is Tightly Packed to Repress Gene Expression via Interaction of HP1 and Histone H3 Lys9 Methylation
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5.2.1 Position Effect Variegation: A Classical Epigenetic Phenomenon to Understand Chromosome Organization and Gene Expression
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English
Additional Edition:
ISBN 0-12-801327-3
Additional Edition:
ISBN 0-12-801080-0
Language:
English
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