UID:
almafu_9960073687102883
Format:
1 online resource (124 p.)
ISBN:
9780128015278
,
0128015276
Content:
Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to disc
Note:
Description based upon print version of record.
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Front Cover; Basic Molecular Protocols in Neuroscience; Copyright Page; Contents; Introduction; 1 General Notes; Aseptic Technique; Aliquoting; 2 DNA and RNA Extraction Protocols; Phenol:Chloroform (TRIzolTM) Extraction Notes; Phenol:Chloroform Extraction Protocol; RNA Extraction; DNA Extraction; Protein Extraction; Kit Extraction of RNA Notes; Solution Recipes; 3 Agarose Gel Electrophoresis; Electrophoresis Notes; Agarose Gel Electrophoresis Protocol; Solution Recipes; 4 Reverse Transcription (RT) and Polymerase Chain Reaction (PCR); Reverse Transcription Notes
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Polymerase Chain Reaction (PCR) NotesEnd-Point PCR; Primer Design; Reverse-Transcription PCR (RT-PCR); Quantitative PCR (Real-Time PCR or qPCR); Quantitation Methods; Running a Plate and Data Analysis; PCR Controls; PCR Troubleshooting; 5 Antibodies and Titrations; 6 Protein Extraction; Protein Extraction Notes; Total Cytosolic/Membrane Protein Extraction Protocol; Subcellular Fractionation Protocol (Adapted from Two Papers); Subcellular Fractionation Protocol (Detergents); Fractionation, Enriched in Neurons and Glia; Fractionation of P1 Pellet (Microvessel-Enriched Fraction)
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Solution Recipes7 Immunoblotting (Western Blot); Immunoblotting Notes; SDS-PAGE and Immunoblot Protocols; SDS-PAGE and Membrane Transfer Protocol; Immunoblotting Protocol; Fluorescence; Chemiluminescence; PVDF Blot Stripping Protocol; Blot Stripping Protocol (Alternative Stripping Buffer); Coomassie Blue Protocol; Fast Green/Ponceau S Protocol; Dot Blot Protocol; Immunoblot Controls; Immunoblot Troubleshooting; Solution Recipes; 8 Immunoprecipitation; Immunoprecipitation Notes; IP Protocol; IP Controls; IP Troubleshooting; Solution Recipes; 9 Perfusion and Immersion Fixation
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Perfusion Fixation NotesTissue Fixation; Tissue Slicing and Storage; Immersion Fixation Protocol; Cell Culture (Grown on Coverslips); Extracted Tissue; Perfusion Fixation Protocol; Sample Storage; Solution Recipes; 10 Immunohistochemistry; Immunohistochemistry Notes; Types of IHC Staining; Secondary Antibodies and Blocking Buffers; Amplification of the Signal; Multiple Labeling; Antibody Titration Protocol (Ni-DAB); Antibody Titration Protocol (Immunofluorescence); IHC Protocol; Cresyl Violet Counterstain (Nissl Stain) Protocol; IHC Controls; IHC Troubleshooting; Solution Recipes
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11 In Situ HybridizationIn Situ Hybridization Notes; Types of ISH Probe Labeling; Visualizing Your ISH; ISH Probe Design for Oligonucleotide and PCR Probes; ISH Protocol (Developed from Two Sources); ISH Controls; Solution Recipes; References
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English
Additional Edition:
ISBN 9780128014615
Additional Edition:
ISBN 012801461X
Language:
English
Keywords:
Handbooks and manuals.
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Handbooks, manuals, etc.
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Handbooks, manuals, etc.
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Handbooks and manuals.
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