Umfang:
1 Online-Ressource (XXII, 632 Seiten, 20 Tafeln)
,
Illustrationen
Ausgabe:
First edition
Ausgabe:
Online-Ausg.
ISBN:
9780128021712
Serie:
Methods in enzymology volume 570
Inhalt:
Front Cover -- Chemokines -- Copyright -- Contents -- Contributors -- Preface -- Chapter One: Chemokine Detection Using Receptors Immobilized on an SPR Sensor Surface -- 1. Surface Plasmon Resonance -- 2. Chemokine Receptors: Members of the GPCR Family -- 3. Chemokine Receptor Immobilization on the Sensor Chip -- 4. Viral Particles as Chemokine Receptors Carriers in SPR -- 4.1. Materials -- 4.2. Method I: Generation of Retroviral Particles -- 4.3. Method II: Generation of LVPs -- 4.4. Method III: Titration of Viral Proteins
Inhalt:
4.5. Method IV: Determination of Chemokine Receptor Levels on the VP -- 4.6. Method V: Quantitation of Receptor Number on VPs -- 4.7. Method VI: Attachment of VPs to Biosensor Surfaces -- 5. SPR-Based Applications for Chemokine Receptors -- 6. Conclusions -- Acknowledgments -- References -- Chapter Two: Study of Chemotaxis and Cell-Cell Interactions in Cancer with Microfluidic Devices -- 1. Introduction -- 2. Methods -- 2.1. Chemotaxis in Microfluidic Chemotaxis Chamber -- 2.1.1. Making Microfluidic Chemotaxis Chamber -- 2.1.2. Chemotaxis Assay for Differentiated HL-60 Cells
Inhalt:
2.2. Gradient Switching in Microfluidic Device -- 2.2.1. Making a Gradient Switching Microfluidic Device -- 2.2.2. Chemotaxis Assay for Differentiated HL-60 Cells with Gradient Switch -- 2.2.2.1. Cell Tracking and Data Analysis -- 2.3. Cell-Cell Interaction in Tumor Microenvironment -- 2.3.1. Creation of the Microfluidic Device -- 3. Limitations -- 4. Perspectives -- 4.1. Shearing Force and Calculation of Chemotactic Force -- 4.2. Cell Polarity Change Versus Cell Turning During a Gradient Change of Direction
Inhalt:
4.3. Potential Future Uses of Microfluidic Devices: Analysis of Circulating Tumor Cells -- 4.4. Advantages of Using 3D Microbioreactors to Investigate Factors that Influence the Tumor Microenvironment -- Acknowledgments -- References -- Chapter Three: Generating Chemokine Analogs with Enhanced Pharmacological Properties Using Phage Display -- 1. Introduction -- 1.1. Chemokines in Health and Disease -- 1.2. Chemokine Structure and Activity -- 1.3. Applying Phage Display Technology to Chemokine Receptors -- 2. Methods -- 2.1. Library Design and Construction -- 2.1.1. Choice of Phage Display System
Inhalt:
2.1.2. Introducing Library Diversity -- 2.1.3. How Much Diversity Is Feasible? -- 2.1.4. Partial Diversity -- 2.1.5. Chemokine Walking: Exploring Diversity Step by Step -- 2.1.6. Required Materials -- 2.1.7. Library Construction -- 2.2. Selection of Libraries on Cells -- 2.2.1. Considerations -- 2.2.2. Cell Lines -- 2.2.3. Required Materials -- 2.2.4. Option 1: Selection for Internalizing Ligands -- 2.2.5. Option 2: Selection for High-Affinity Cell Surface Binding -- 3. Limitations -- 3.1. The Atypical Chemokine Receptor DARC (ACKR1) -- 4. Perspectives
Inhalt:
4.1. A Chemokine-Based HIV Prevention Strategy
Anmerkung:
Description based upon print version of record
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe Handel, Tracy Chemokines : Elsevier Science,c2016
Sprache:
Englisch
Fachgebiete:
Biologie
Schlagwort(e):
Chemokine
;
Biochemische Analyse
;
Untersuchungsmethode
;
Electronic books
;
Aufsatzsammlung
URL:
Volltext
(URL des Erstveröffentlichers)
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