UID:
almafu_9958128873902883
Umfang:
1 online resource (760 p.)
ISBN:
9780128023310
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0128023317
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9780128021927
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0128021926
Serie:
Methods in Enzymology, Volume 560
Inhalt:
〈i〉 RNA Modification〈/i〉 provides a useful examination of the science and its role in biological regulation, the current frontier of life science research, and includes various RNA modications and their role in gene expression. It represents the most up-to-date knowledge and protocols available today. 〈br〉〈br〉〈ul〉〈li〉Dynamic RNA modifications and their roles in biological regulation are the current frontier of life science research〈/li〉〈li〉This volume of Methods in Enzymology represents up to date knowledge and protocols〈/li〉〈/ul〉
Anmerkung:
Description based upon print version of record.
,
Cover image; Title page; Table of Contents; Copyright; Contributors; Preface; Chapter One: Methodology for the High-Throughput Identification and Characterization of tRNA Variants That Are Substrates for a tRNA Decay Pathway; Abstract; 1 Introduction; 2 High-Throughput Identification of tRNA Substrates Degraded by the RTD Pathway; 3 Measurement of tRNA Levels of RTD Substrate SUP4oc Variants in the Presence of WT tRNATyr; 4 Conclusions and Additional Applications of These Approaches; Acknowledgments
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Chapter Two: Nucleoside Analysis by Hydrophilic Interaction Liquid Chromatography Coupled with Mass SpectrometryAbstract; 1 Introduction; 2 Nucleoside Preparation for LC/MS Analysis; 3 HILIC/ESI-MS for Total Nucleosides; 4 HILIC/ESI-MS Versus RPC/ESI-MS for Nucleoside Analysis; 5 Profiling of Modified Nucleosides in HILIC/ESI-MS; 6 Discussion; Acknowledgments; Chapter Three: A Platform for Discovery and Quantification of Modified Ribonucleosides in RNA: Application to Stress-Induced Reprogramming of tRNA Modifications; Abstract; 1 Introduction; 2 Methods; 3 Discussion; Acknowledgments
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Chapter Four: Recognition of Specified RNA Modifications by the Innate Immune SystemAbstract; 1 Introduction; 2 Preparation of tRNA Modivariants; 3 Preparation and Stimulation of Human PBMC; 4 Preparation of Human PBMCs; 5 Stimulation of Human PBMC; Acknowledgment; Chapter Five: Kinetic Analysis of tRNA Methyltransferases; Abstract; 1 Introduction; 2 Methodology; 3 Steady-State Assays; 4 Pre-Steady-State Assays; 5 Single-Turnover Assays; 6 Conclusions; Acknowledgments
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Chapter Six: Preparation of Human Nuclear RNA m6A Methyltransferases and Demethylases and Biochemical Characterization of Their Catalytic ActivityAbstract; 1 Introduction; 2 Expression of Human Nuclear RNA m6A Methyltransferase Core Complex METTL3-METTL14-WTAP in Insect Cell Expression System; 3 Expression of Human Nuclear RNA m6A Demethylases FTO and ALKBH5; 4 Biochemical Characterization of the Catalytic Activity of the m6A Methyltransferases and Demethylases; 5 Conclusions; Acknowledgments; Chapter Seven: Transcriptome-Wide Mapping of N6-Methyladenosine by m6A-Seq; Abstract; 1 Theory
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2 Equipment3 Materials; 4 Protocol; 5 Step 1: RNA Preparation and Fragmentation; 6 Step 2: RNA Immunoprecipitation; 7 Step 3: Library Preparation and Deep Sequencing; Acknowledgments; Chapter Eight: Probing RNA Modification Status at Single-Nucleotide Resolution in Total RNA; Abstract; 1 Introduction; 2 Methods; 3 Notes; Acknowledgments; Chapter Nine: High-Resolution Mapping of N6-Methyladenosine in Transcriptome and Genome Using a Photo-Crosslinking-Assisted Strategy; Abstract; 1 Introduction; 2 Equipment; 3 Materials; 4 Protocol 1: Photo-Crosslinking-Assisted m6A Sequencing
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5 Protocol 2: 6mA-CLIP-exo Sequencing
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English
Sprache:
Englisch
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