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Format: 1 online resource (474 p.)

Edition: First edition.

ISBN: 0-12-811123-2 , 0-12-804539-6

Series Statement: Methods in enzymology ; volume five hundred and seventy six

Note: Includes index. , Front Cover; Synthetic Biology and Metabolic Engineering in Plants and Microbes Part B: Metabolism in Plants; Copyright; Contents; Contributors; Preface; Chapter One: Comparative Biochemistry and In Vitro Pathway Reconstruction as Powerful Partners in Studies of Metabolic Di ...; 1. Introduction; 2. Characterization of Specialized Metabolite Diversity Using LC/MS and NMR; 2.1. Metabolite Structure Elucidation by LC/MS with Multiplexed CID; 2.1.1. Example of Acylsugar Structure Profiling by LC/MS; 2.1.2. Protocol for Plant Trichome Acylsugar Extraction and LC/MS , 2.2. Sample Purification for NMR Spectroscopy2.2.1. Protocol for Purifying Individual Acylsugars from Trichomes for NMR Analysis; 3. Phylogeny-Driven Analysis of the Biochemical Basis of Specialized Metabolite Diversity; 3.1. Phylogeny-Based Screening of Metabolic Phenotypes; 3.2. The Biochemical Basis for Differences in Furanose Ring Acylation; 3.2.1. Protocol to Clone and Characterize S. habrochaites ASAT3 Isoforms; 3.3. Identifying a Key Polymorphism Driving Enzyme Function Divergence; 3.3.1. Discovery of an ASAT3 Polymorphism Associated with In Vitro Functional Variation , 3.3.2. Protocol Used to Identify Key Polymorphisms Contributing to Variation in ASAT3 Acyl-CoA Substrate Specificity4. Conclusions; Acknowledgments; References; Chapter Two: De Novo Deep Transcriptome Analysis of Medicinal Plants for Gene Discovery in Biosynthesis of Plant Natural ...; 1. Methods for Transcriptomic Study Prior to High-Throughput Sequencing; 2. Deep Transcriptome Outline; 3. Preparation of the Plant Materials and RNA Extraction; 4. cDNA Library Construction; 5. High-Throughput Sequencing; 6. Data Interpretation; 6.1. Trimming and Quality Validation of Raw Reads , 6.2. De Novo Assembly6.3. Expression Analysis of Transcripts; 6.4. Sequence Similarity Search; 6.5. Gene Annotation; 6.6. An Example of RNA-Seq; 7. Application of RNA-Seq on Medicinal Plants and Perspectives; Acknowledgments; References; Chapter Three: Genomics-Based Discovery of Plant Genes for Synthetic Biology of Terpenoid Fragrances: A Case Study in San ...; 1. Introduction; 2. Prior Knowledge of Sandalwood TPSs and P450s and Development of a Hypothesis; 3. Replication, Sampling, and Statistical Design; 4. Defining Temporal and Spatial Variables for Tissue Sampling; 5. Tissue Sampling , 6. Metabolite Profiling7. Isolation of High-Quality RNA from Recalcitrant Tissues; 8. Transcriptome Sequencing and De Novo Assembly; 9. Transcriptome Mining and Annotation; 10. Expression Analysis and Candidate Gene Selection; 11. Functional Characterization of Candidate Genes; 11.1. Yeast In Vivo Assays; 11.2. Microsomes In Vitro Assays; 12. Product Identification; 12.1. MS-MS; 12.2. Nuclear Magnetic Resonance; Acknowledgments; References; Chapter Four: A Workflow for Studying Specialized Metabolism in Nonmodel Eukaryotic Organisms; 1. Introduction , 2. ``Omics-́́Based Novel Specialized Metabolic Pathway Discovery

Language: English

UID:

Format: 1 Online-Ressource (xviii, 428 Seiten)

Edition: First edition

ISBN: 9780128111239

Series Statement: Methods in enzymology volume 576

Content: Synthetic Biology and Metabolic Engineering in Plants and Microbes, Part B, the latest volume in the Methods in Enzymology series,continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods, synthetic biology, and metabolic engineering in plants and microbes, and includes sections on such topics as the usage of integrases in microbial engineering, biosynthesis, and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways.Continues the legacy of this premier serial with quality chapters authored by leaders in the field of enzymologyContains two volumes covering research methods in synthetic biology and metabolic engineering in plants and microbesIncludes sections on such topics as the uses of integrases in microbial engineering, biosynthesis and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways

Note: Description based upon print version of record

Additional Edition: ISBN 9780128045398

Additional Edition: Erscheint auch als Druck-Ausgabe O'Connor, Sarah E Synthetic Biology and Metabolic Engineering in Plants and Microbes Part B: Metabolism in Plants San Diego : Elsevier Science,c2016 ISBN 9780128045398

Language: English

Keywords: Synthetische Biologie ; Mikroorganismus ; Pflanzen ; Stoffwechsel ; Electronic books

URL: Volltext

UID:

Format: 1 online resource (474 p.)

Edition: First edition.

ISBN: 0-12-811123-2 , 0-12-804539-6

Series Statement: Methods in enzymology ; volume five hundred and seventy six

Note: Includes index. , Front Cover; Synthetic Biology and Metabolic Engineering in Plants and Microbes Part B: Metabolism in Plants; Copyright; Contents; Contributors; Preface; Chapter One: Comparative Biochemistry and In Vitro Pathway Reconstruction as Powerful Partners in Studies of Metabolic Di ...; 1. Introduction; 2. Characterization of Specialized Metabolite Diversity Using LC/MS and NMR; 2.1. Metabolite Structure Elucidation by LC/MS with Multiplexed CID; 2.1.1. Example of Acylsugar Structure Profiling by LC/MS; 2.1.2. Protocol for Plant Trichome Acylsugar Extraction and LC/MS , 2.2. Sample Purification for NMR Spectroscopy2.2.1. Protocol for Purifying Individual Acylsugars from Trichomes for NMR Analysis; 3. Phylogeny-Driven Analysis of the Biochemical Basis of Specialized Metabolite Diversity; 3.1. Phylogeny-Based Screening of Metabolic Phenotypes; 3.2. The Biochemical Basis for Differences in Furanose Ring Acylation; 3.2.1. Protocol to Clone and Characterize S. habrochaites ASAT3 Isoforms; 3.3. Identifying a Key Polymorphism Driving Enzyme Function Divergence; 3.3.1. Discovery of an ASAT3 Polymorphism Associated with In Vitro Functional Variation , 3.3.2. Protocol Used to Identify Key Polymorphisms Contributing to Variation in ASAT3 Acyl-CoA Substrate Specificity4. Conclusions; Acknowledgments; References; Chapter Two: De Novo Deep Transcriptome Analysis of Medicinal Plants for Gene Discovery in Biosynthesis of Plant Natural ...; 1. Methods for Transcriptomic Study Prior to High-Throughput Sequencing; 2. Deep Transcriptome Outline; 3. Preparation of the Plant Materials and RNA Extraction; 4. cDNA Library Construction; 5. High-Throughput Sequencing; 6. Data Interpretation; 6.1. Trimming and Quality Validation of Raw Reads , 6.2. De Novo Assembly6.3. Expression Analysis of Transcripts; 6.4. Sequence Similarity Search; 6.5. Gene Annotation; 6.6. An Example of RNA-Seq; 7. Application of RNA-Seq on Medicinal Plants and Perspectives; Acknowledgments; References; Chapter Three: Genomics-Based Discovery of Plant Genes for Synthetic Biology of Terpenoid Fragrances: A Case Study in San ...; 1. Introduction; 2. Prior Knowledge of Sandalwood TPSs and P450s and Development of a Hypothesis; 3. Replication, Sampling, and Statistical Design; 4. Defining Temporal and Spatial Variables for Tissue Sampling; 5. Tissue Sampling , 6. Metabolite Profiling7. Isolation of High-Quality RNA from Recalcitrant Tissues; 8. Transcriptome Sequencing and De Novo Assembly; 9. Transcriptome Mining and Annotation; 10. Expression Analysis and Candidate Gene Selection; 11. Functional Characterization of Candidate Genes; 11.1. Yeast In Vivo Assays; 11.2. Microsomes In Vitro Assays; 12. Product Identification; 12.1. MS-MS; 12.2. Nuclear Magnetic Resonance; Acknowledgments; References; Chapter Four: A Workflow for Studying Specialized Metabolism in Nonmodel Eukaryotic Organisms; 1. Introduction , 2. ``Omics-́́Based Novel Specialized Metabolic Pathway Discovery

Language: English