UID:
almahu_9949985097902882
Umfang:
1 online resource (466 pages)
Ausgabe:
First edition.
ISBN:
9780443264719
,
0443264716
Inhalt:
This book, 'Natural Biomaterials for Tissue Engineering,' edited by Naveen Kumar and others, explores the development and application of biomaterials derived from natural sources for use in tissue engineering. The book covers a wide range of topics including definitions, features, and crosslinking of biomaterials, as well as the preparation and evaluation of extracellular matrix scaffolds derived from various animal tissues such as rumen, reticulum, gallbladder, aorta, and fish swim bladder. It also discusses the use of stem cells and bioactive scaffolds for regenerative medicine. The book is intended for researchers, practitioners, and students in the fields of veterinary science, medical research, and biomedical engineering, aiming to provide comprehensive insights into the latest advancements and methodologies in tissue engineering.
Anmerkung:
Front Cover -- Natural Biomaterials for Tissue Engineering -- Copyright Page -- Contents -- List of contributors -- Preface -- 1 An introduction to biomaterials -- 1.1 Introduction -- 1.2 Definitions of biomaterials -- 1.3 Basic features required for the biomaterial -- 1.3.1 Characteristics of biomaterials -- 1.3.2 Requirements for scaffolds used in tissue engineering -- 1.4 Classification of biomaterials -- 1.5 Biocompatibility -- 1.6 Summary -- References -- 2 Decellularization and characterization methods -- 2.1 Introduction -- 2.2 Decellularization -- 2.2.1 Cellular matrix acellular matrix -- 2.3 Decellularized biomaterials -- 2.4 Characterization methods of decellularized tissues -- 2.4.1 Hematoxylin and eosin -- 2.4.2 4',6-Diamidino-2-phenylindole (DAPI) -- 2.4.3 The MTT cell proliferation assay -- 2.4.4 Second harmonic generation (SHG) -- 2.4.5 Electron microscopy -- 2.4.5.1 Transmission electron microscopy (TEM) -- 2.4.5.2 Scanning electron microscopy (SEM) -- 2.4.6 Mechanical properties -- 2.4.7 Zymography -- 2.5 Development of decellularized naturally derived biomaterials -- 2.6 In vitro evaluation of decellularized naturally derived biomaterials -- References -- 3 Crosslinking of biomaterials -- 3.1 Introduction -- 3.2 Advantages of crosslinking biomaterials -- 3.3 Disadvantages of crosslinking biomaterials -- 3.4 Types of crosslinking -- 3.4.1 Chemical crosslinking -- 3.4.1.1 Advantages of chemical crosslinking -- 3.4.1.2 Disadvantages of chemical crosslinking -- 3.4.2 Physical crosslinking -- 3.4.2.1 Advantages of physical crosslinking -- 3.4.2.2 Disadvantages of physical crosslinking -- 3.4.3 Biological crosslinking -- 3.4.3.1 Advantages of biological crosslinking -- 3.4.3.2 Disadvantages of biological crosslinking -- 3.5 Crosslinking agents -- 3.5.1 Glutaraldehyde -- 3.5.2 Formaldehyde -- 3.5.3 Glyoxal.
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3.5.4 Diphenyl phosphoryl azide -- 3.5.5 Ethylene glycol diglycidyl ether -- 3.5.6 Polyethylene glycol -- 3.5.7 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride -- 3.5.8 Hexamethylene diisocyanate -- 3.5.9 Physical -- 3.5.10 Enzymatic -- References -- 4 Rumen-derived extracellular matrix scaffolds and clinical application -- 4.1 Introduction -- 4.2 Anatomy of ruminant stomach -- 4.3 Decellularization of forestomach matrix -- 4.4 Preparation of acellular matrices from bubaline rumen -- 4.4.1 Macroscopic observations -- 4.4.2 Microscopic observations -- 4.4.2.1 Treatment with zwitter ionic detergent (tri-N-butyl phosphate) -- 4.4.2.2 Treatment with nonionic detergent (triton X-100) -- 4.4.2.3 Treatment with ionic detergent (sodium dodecyl sulfate) -- 4.4.2.4 Treatment with nonionic detergent (tween-20) -- 4.4.2.5 Treatment with enzyme (trypsin) -- 4.4.3 DNA quantification -- 4.4.4 Cytocompatibility analysis -- 4.5 Development of 3-D bioengineered scaffolds from bubaline rumen -- 4.6 Testing the efficacy of 3-D bioengineered scaffolds in a diabetic rat model -- 4.7 Evaluation of bubaline rumen matrix in clinical cases -- 4.7.1 Surgical technique -- 4.7.2 Gross and clinical observations -- 4.8 Preparation of acellular matrices from caprine rumen -- 4.8.1 Microscopic observations -- 4.8.2 DNA quantification -- 4.8.3 SDS-PAGE analysis -- 4.8.4 Calorimetric protein estimation -- 4.8.5 Scanning electron microscopic observations -- 4.9 Evaluation of caprine rumen matrix in clinical cases -- 4.10 Conclusions -- References -- 5 Reticulum-derived extracellular matrix scaffolds -- 5.1 Introduction -- 5.2 Preparation of acellular bovine reticulum extracellular matrix -- 5.2.1 Gross and microscopic observations -- 5.2.2 Scanning electron microscopic observations -- 5.2.3 DNA contents analysis.
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5.3 Preparation of acellular caprine reticulum extracellular matrix -- 5.3.1 Microscopic observations -- 5.3.2 DNA contents analysis -- 5.3.3 Calorimetric protein estimation -- 5.3.4 Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis -- 5.4 Evaluation of bovine reticulum extracellular matrix in rat model -- 5.4.1 Wound area and percent contraction -- 5.4.2 Gross observations -- 5.4.3 Hematological observations -- 5.4.4 Immunological observations -- 5.4.5 Histopathological observations -- 5.5 Conclusion -- References -- 6 Omasum-derived extracellular matrix scaffolds -- 6.1 Introduction -- 6.2 Preparation of acellular matrix from buffalo omasum -- 6.2.1 Gross observations -- 6.2.2 Microscopic observations -- 6.2.3 DNA quantification -- 6.2.4 Sodium dodecyl sulfate polyacrylamide gel electrophoresis -- 6.3 Preparation of acellular matrix from goat omasum -- 6.3.1 Microscopic observations -- 6.3.2 DNA quantification -- 6.3.3 Calorimetric protein estimation -- 6.3.4 Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis -- 6.4 Experimental evaluation of acellular buffalo omasum in wound healing in rat model -- 6.4.1 Animals and ethics statement -- 6.4.2 Skin wound creation and implantation -- 6.4.3 Wound area and wound contraction -- 6.4.4 Gross observations/planimetry -- 6.4.5 Immunological observations -- 6.4.5.1 Humoral response -- 6.4.5.2 Cell-mediated immune response -- 6.4.6 Histological observations -- 6.4.6.1 Group I (open wound) -- 6.4.6.2 Group II (wound with commercially available collagen sheet) -- 6.4.6.3 Group III (wound with acellular buffalo omasum laminae) -- 6.5 Conclusion -- References -- 7 Gall bladder-derived extracellular matrix scaffolds -- 7.1 Introduction -- 7.2 Anatomy of gallbladder (cholecyst) -- 7.3 Cholecyst-derived extracellular matrix.
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7.4 Preparation of acellular buffalo cholecyst-derived extracellular matrix -- 7.4.1 Macroscopic observations -- 7.4.2 Microscopic observations -- 7.4.3 Scanning electron microscopic observations -- 7.4.4 DNA contents analysis -- 7.5 Preparation of acellular pig cholecyst-derived extracellular matrix -- 7.5.1 Macroscopic observations -- 7.5.2 Microscopic observations -- 7.5.3 Scanning electron microscopic observations -- 7.5.4 DNA contents analysis -- 7.6 Preparation of acellular goat cholecyst-derived extracellular matrix -- 7.6.1 Preparation of soapnut pericarp extract -- 7.6.2 Preparation of acellular goat gall bladder matrix -- 7.6.3 Microscopic observations -- 7.6.4 DAPI staining: (4, 6-diamidino-2-phenylindole 2Hcl) -- 7.6.5 Scanning electron microscopic observations -- 7.6.6 DNA contents analysis -- 7.7 Experimental evaluation of buffalo cholecyst-derived extracellular matrix in a rat model -- 7.7.1 Animals and ethics statement -- 7.7.2 Skin wound creation and implantation -- 7.7.3 Wound contraction -- 7.7.4 Gross observations/planimetry -- 7.7.5 Immunological observations -- 7.7.5.1 Humoral response -- 7.7.5.2 Cell-mediated immune response -- 7.7.6 Histopathological observations -- 7.8 Experimental evaluation of pig cholecyst-derived extracellular matrix in a rat model -- 7.8.1 Skin wound creation and implantation -- 7.8.2 Wound contraction -- 7.8.3 Gross observations/planimetry -- 7.8.4 Immunological observations -- 7.8.4.1 Humoral response -- 7.8.4.2 Cell-mediated immune response -- 7.8.5 Histological observations -- 7.9 Conclusion -- References -- 8 Aorta-derived extracellular matrix scaffolds and clinical application -- 8.1 Introduction -- 8.2 Optimization of protocols for decellularization of buffalo aorta -- 8.2.1 Group A -- 8.2.2 Group B -- 8.2.3 Optimization of protocols for preparation of acellular aortic matrix -- 8.2.4 Group A.
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8.2.5 Protocol A1 -- 8.2.6 Protocol A2 -- 8.2.7 Protocol A3 -- 8.2.8 Protocol A4 -- 8.2.9 Protocol A5 -- 8.2.10 Protocol A6 -- 8.2.11 Group B -- 8.2.12 Protocol B1 -- 8.2.13 Protocol B2 -- 8.2.14 Protocol B3 -- 8.2.15 Protocol B4 -- 8.2.16 Protocol B5 -- 8.2.17 Protocol B6 -- 8.3 Preparation and characterization of the buffalo aortic matrix -- 8.3.1 Histological observations -- 8.3.2 Scanning electron microscopic (SEM) observations -- 8.3.3 DNA extraction, quantification, and purity -- 8.3.4 Fourier transform infrared (FTIR) spectroscopy -- 8.4 In vivo biocompatibility determination of acellular aortic matrix -- 8.4.1 Surgical procedure -- 8.4.2 Macroscopic observations -- 8.4.3 Microscopic observations -- 8.4.4 Immunological studies -- 8.4.5 Lymphocyte proliferation assay -- 8.4.5.1 Preparation of antigen -- 8.4.5.2 Peripheral blood lymphocytes -- 8.4.5.3 Splenocytes culture -- 8.4.5.4 ELISA -- 8.4.6 Molecular weight analysis -- 8.5 Clinical applications in different species of animals -- 8.6 Conclusion -- References -- 9 Pericardium-derived extracellular matrix scaffolds -- 9.1 Introduction -- 9.2 Preparation of acellular goat pericardium matrix -- 9.3 Crosslinking of native and acellular goat pericardium matrix -- 9.4 Preparation of acellular buffalo pericardium matrix -- 9.5 Crosslinking of native and acellular buffalo pericardium matrix -- 9.5.1 Gross observations -- 9.5.1.1 Concentration of solution -- 9.5.1.2 Duration of treatment -- 9.5.1.3 Temperature -- 9.5.2 In vitro enzymatic degradation -- 9.5.2.1 In vitro collagenase enzymatic degradation -- 9.5.2.2 In vitro elastase enzymatic degradation -- 9.5.2.3 In vitro trypsin enzymatic degradation -- 9.5.3 Free amino group contents determination -- 9.5.4 Moisture content analysis -- 9.5.5 Molecular weight analysis -- 9.5.6 In vitro cell cytotoxicity -- 9.6 In vivo evaluation in a rabbit model.
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9.6.1 Macroscopic observations.
Weitere Ausg.:
ISBN 9780443264702
Weitere Ausg.:
ISBN 0443264708
Sprache:
Englisch
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