UID:
almafu_9958127046402883
Format:
1 online resource (380 p.)
Edition:
1st ed.
ISBN:
9786613239365
,
9781283239363
,
1283239361
,
9780470669822
,
0470669829
,
9780470669815
,
0470669810
Series Statement:
Essential Methods
Content:
This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible.This book is
Note:
Description based upon print version of record.
,
CONTENTS; Contributors; Preface; Abbreviations; Protocols; 1 The Cell Culture Laboratory; 1.1 Introduction; 1.2 Methods and approaches; 1.3 Troubleshooting; Acknowledgements; References; 2 Sterilization; 2.1 Introduction; 2.2 Methods and approaches; 2.3 Troubleshooting; References; 3 Microscopy of Living Cells; 3.1 Introduction; 3.2 Methods and approaches; 3.3 Troubleshooting; Acknowledgements; References; 4 Basic Techniques and Media, the Maintenance of Cell Lines,and Safety; 4.1 Introduction; 4.2 Methods and approaches; 4.3 Troubleshooting; Acknowledgements; References
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5 Development and Optimization of Serum- and Protein-freeCulture Media5.1 Introduction; 5.2 Methods and approaches; 5.3 Troubleshooting; 5.4 Conclusion; Acknowledgements; References; 6 Cryopreservation and Banking of Cell Lines; 6.1 Introduction; 6.2 Methods and approaches; 6.3 Troubleshooting; Acknowledgements; References; 7 Primary Culture of Specific Cell Types and the Establishmentof Cell Lines; 7.1 Introduction; 7.2 Methods and approaches; 7.3 Troubleshooting; References; 8 Cloning; 8.1 Introduction; 8.2 Methods and approaches; 8.3 Troubleshooting; References
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9 The Quality Control of Animal Cell Lines and the Prevention,Detection and Cure of Contamination9.1 Introduction; 9.2 Methods and approaches; 9.3 Troubleshooting; References; 10 Systems for Cell Culture Scale-up; 10.1 Introduction; 10.2 Methods and approaches; 10.3 Troubleshooting; References; 11 Good Laboratory Practice in the Cell Culture Laboratory; 11.1 Introduction; 11.2 Background to GLP; 11.3 General GLP principles; 11.4 Study performance; 11.5 Good Manufacturing Practice; 11.6 Summary; References; Index
,
English
Additional Edition:
ISBN 9780470975633
Additional Edition:
ISBN 0470975636
Additional Edition:
ISBN 9780470666586
Additional Edition:
ISBN 0470666587
Language:
English
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