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Format: 1 online resource (XVIII, 699 p. 192 illus., 110 illus. in color.)

Edition: 1st ed. 2020.

ISBN: 9781071604304 , 1071604309

Series Statement: Methods in Molecular Biology, 2132

Content: This volume presents a series of experimental protocols for glycan analysis using lectins and the functional analysis of lectins. A key feature in this book are two comprehensive overview chapters on lectin structures and a list of the all the lectins—these chapters discuss the latest findings in the field. The remainder of the book covers topics such as lectins from mammals, non-mammalian animals; plants, algae, fungi, protists, bacteria, and viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Lectin Purification and Analysis: Methods and Protocols is a valuable tool for any scientist interested in learning more about these life systems.

Note: Structural database for Lectins and the UniLectin Web Platform -- Purification of Sugar-Binding Peptides from L-Type Lectins -- Recombinant Expression and Purification of Animal Intracellular L-Type Lectins -- Frontal Affinity Chromatography: A Highly Suitable Retardation Phenomenon-Based research Tool for Analyzing Weak Interactions between Biomolecules -- Metazoan Soluble b-Galactoside-Binding Lectins, Galectins: Methods for Purification, Characterization of their Carbohydrate Binding Specificity, and probing their Ligands -- Expression, S-Nitrosylation, and Measurement of S-Nitrosylation Ratio of Recombinant Galectin-2 -- Expression and Purification of Full-Length and Domain-Fragment Recombinant Pentraxin 3 (PTX3) Proteins from Mammalian and Bacterial Cells -- Identification of Siglec Cis-Ligands by Proximity Labeling -- Preparation of Recombinant Siglecs and Identification of their Ligands -- Purification, Quantification, and FunctionalAnalysis of Collectins -- Selectin-Binding Assay by Flow-Cytometry -- Direct Binding Analysis between C-Type Lectins and Glycans Using Immunoglobulin-Receptor Fusion Proteins -- Expression and Characterization of Hyaluronan Binding Protein Involved in Hyaluronan Depolymerization: HYBID, alias KIAA1199 and CEMIP -- Paracoccin: Purification and validation of its Lectin and Enzymatic Properties -- In Vitro Mannosidase Assay of EDEMs: ER Degradation-Enhancing b-Mannosidase-Like Proteins -- Galactose-Specific, Hemolytic Lectin CEL-III from Cucumaria echinata -- Simple and Rapid Detection of Glycoforms by “Lectin Inhibition” Assay -- Glycoform-Specific Visualization in Immunohistochemistry by “Lectin Inhibition” -- Botulinum Hemagglutinin: Critical protein for Adhesion and Absorption of Neurotoxin Complex in Host Intestine -- Functional Analysis of Botulinum Hemagglutinin (HA) -- Purification and Functional Characterization of the Effects onCell Signaling of Mytilectin: A Novel β-Trefoil Lectin from Marine Mussels -- Lectin-Type Ubiquitin Ligase Subunits: Fbs Proteins and their Applications for Use -- F-Type Lectins: Structure, Function, and Evolution -- Purification and Biochemical Characterization of Selected F-Type Lectins -- LecA (PA-IL): A Galactose-Binding Lectin from Pseudomonas aeruginosa -- Glycan Recognition and Application of P-Type Lectins -- Purification and Assays of Tachylectin-5 -- Preparation of Soluble Malectin and its Tetramer -- Calnexin/Calreticulin and Assays Related to N-Glycoprotein Folding In Vitro -- Purification and Assays of Tachylectin-2 -- Purification and Assays of Tachycitin -- Methods for Purifying Datura stramonium Agglutinin and Producing Recombinant Agglutinin Protein in a Heterologous Plant Host -- ZG16p, An Animal Homologue of Plant β-Prism Fold Lectins: Purification Methods of Natural and Recombinant ZG16p and Inhibition Assay of CancerCell Growth using ZG16p -- ArtinM: Purification and Evaluation of Biological Activities -- Bacterial Expression of Rhamnose-Binding Lectin from Catfish Eggs -- A Bioassay for Determining Symbiotic Zooxanthellae Shape Control Using Lectin SLL-2 from the Octocoral Sinularia lochmodes -- MIC4 from Toxoplasma gondii: A Lectin Acting as a Toll-Like Receptor Agonist -- Production and Characterization of MIC1: A Lectin from Toxoplasma gondii -- Affinity Labeling and Purification of Plant Chitin-Binding LysM-Receptor with Chitin Octasaccharide Derivatives -- Purification of GNA-Related Lectins from Natural Sources -- Expression, Purification, and Applications of the Recombinant Lectin PVL from Psathyrella velutina Specific for Terminal N-Acetyl-Glucosamine -- Yeast Flocculin: Methods for Quantitative Analysis of Flocculation in Yeast Cells -- Pleurotus cornucoiae Mycelial Lectin (PCL-M): Purification and Detection of the Acitivty on Mycelial Surface -- Expression and Purification of a Human Pluripotent Stem Cell-Specific Lectin Probe, rBC2LCN -- Preparation of Fluorescent Recombinant Shiga Toxin-B-Subunit and its Application to Flow Cytometry -- LecB, A High Affinity Soluble Fucose-Binding Lectin from Pseudomonas aeruginosa -- Sialoglycovirology of Lectins: Sialyl Glycan Binding of Enveloped and Non-Enveloped Viruses -- Hemagglutinin Inhibitors are Potential Future Anti-Influenza Drugs for Mono- and Combination-Therapies -- Preparation and Detection of Glycan Binding Activity of Influenza Virus -- Screening for Components/Compounds with Anti-Rotavirus Activity: Detection of Interaction between Viral Spike Proteins and Glycans -- ELISA-Based Methods to Detect and Quantify Norovirus Virus-Like Particle Attachment to Histo-Blood Group Antigens -- FAM3B/PANDER-Like Carbohydrate-Binding Domain in a Glycosyltransferase, POMGNT1 -- Mannose-Specific Oyster Lectin CGL1 -- Receptor Binding Assays of Enterovirus D68 -- Large-Scale Expression and Purification of Mumps Virus Hemagglutinin-Neuraminidase for Structural Analyses and Glycan-Binding Assays -- Purification of AJLec: A Novel Galactose-Specific Lectin, from the Sea Anemone Anthopleura japonica -- Annexin Lectins: Ca2+-Dependent Heparin-Binding Activity, Phosphatidylserine-Binding Activity, and Anticoagulant Activity -- Expression, Purification, and Functional Characterization of Tectonin 2 from Laccaria bicolor: A Six-Bladed Beta-Propeller Lectin Specific for O-Methylated Glycans -- The OAAH Family: Anti-Influenza Virus Lectins.

Additional Edition: ISBN 9781071604298

Additional Edition: ISBN 1071604295

Language: English

Keywords: Llibres electrònics ; Llibres electrònics

DOI: 10.1007/978-1-0716-0430-4