UID:
almafu_9958132554702883
Format:
1 online resource (663 p.)
ISBN:
9786611011277
,
9781281011275
,
1281011274
,
9780080496986
,
0080496989
Series Statement:
Methods in enzymology ; v. 350
Content:
This volume and its companion, Volume 351, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include basic techniques, making mutants, genomics, and proteomics.
Note:
Description based upon print version of record.
,
Front Cover; Guide to Yeast Genetics and Molecular and Cell Biology; Copyright Page; Table of Contents; Contributors to Volume 350; Preface; Volume in Series; Section I: Basic Techniques; Chapter 1. Getting Started with Yeast; Chapter 2. How to Set up a Yeast Laboratory; Chapter 3. Constructing Yeast Libraries; Chapter 4. Transformation of Yeast by Lithium Acetate/Single-Stranded Carrier DNA/Polyethylene Glycol Method; Chapter 5. Genetic Transformation of Saccharomyces cerevisiae Mitochondria; Chapter 6. LacZ Assays in Yeast; Chapter 7. Analysis of Budding Patterns
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Chapter 8. Uses and Abuses of HO EndonucleaseChapter 9. Assays for Gene Silencing in Yeast; Section II: Making Mutants; Chapter 10. Classical Mutagenesis Techniques; Chapter 11. Introduction of Point Mutations into Cloned Genes; Chapter 12. Insertional Mutagenesis: Transposon-Insertion Libraries as Mutagens in Yeast; Chapter 13. Tn7-Mediated Mutagenesis of Saccharomyces cerevisiae Genomic DNA in Vitro; Chapter 14. Vector Systems for Heterologous Expression of Proteins in Saccharomyces cerevisiae
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Chapter 15. Cloning-Free Genome Alterations in Saccharomyces cerevisiae Using Adaptamer-Mediated PCRChapter 16. kar-Mediated Plasmid Transfer between Yeast Strains: Alternative to Traditional Transformation Methods; Chapter 17. Gene Disruption; Chapter 18. Synthetic Dosage Lethality; Section III: Genomics; Chapter 19. Saccharomyces Genome Database; Chapter 20. Three Yeast Proteome Databases: PombePD, and CalPD (MycoPathPD); Chapter 21. Database Resources Relevant to Yeast Biology; Chapter 22. Searching Yeast Intron Data at Ares Lab Web Site
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Chapter 23. Yeast Genomic Expression Studies Using DNA MicroarraysChapter 24. Transcriptome Analysis of Saccharomyces cerevisiae Using Serial Analysis of Gene Expression; Chapter 25. PCR-Based Engineering of Yeast Genome; Chapter 26. ChIP-chip: A Genomic Approach for Identifying Transcription Factor Binding Sites; Chapter 27. Computational Approaches to Identifying Transcription Factor Binding Sites in Yeast Genome; Section IV: Proteomics; Chapter 28. Array-Based Methods for Identifying Protein Protein and Protein-Nucleic Acid Interactions
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Chapter 29. Building Protein-Protein Networks by Two- Hybrid Mating StrategyChapter 30. Integrated Version of Reverse Two-Hybrid System for the Postproteomic Era; Chapter 31. Biochemical Genomics Approach to Map Activities to Genes; Chapter 32. Use of Two-Dimensional Gels in Yeast Proteomics; Author Index; Subject Index
,
English
Additional Edition:
ISBN 9780121822538
Additional Edition:
ISBN 0121822532
Language:
English
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