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Format: Online-Ressource , v.: digital

Edition: 1

Edition: Online-Ausg. Springer eBook Collection. Biomedical and Life Sciences Electronic reproduction; Available via World Wide Web

ISBN: 9781441912602

Series Statement: Reviews in Fluorescence 2008 2008

Content: This is the fifth volume in the Reviews in Fluorescence series. To date, four volumes have been both published and well received by the scientific community. Several book reviews in the last few years have also favourably remarked on the series. In this 5th volume we continue the tradition of publishing leading edge and timely articles from authors around the world. We thank the authors for their timely and exciting contributions. We hope you will find this volume as useful as past volumes, which promises to be just as diverse with regard to fluorescence-based content

Note: Includes index , Preface; Contents; Contributors; Fluorescence Anisotropy to Study the Preferential Orientation of Fluorophores in Ordered Bi-Dimensional Systems: Rhodamine 6G/Laponite Layered Films; 1 Introduction; 2 Fluorescence Anisotropy in Ordered Bi-Dimensional Systems; 3 Dye/Clay Systems: Film Characterization; 4 Dye Orientation in Ordered Clay Films. A Fluorescence Anisotropy Study; 5 Conclusions; References; Room Temperature Tryptophan Phosphorescence of Proteins in the Composition of Biological Membranes and Solutions; 1 Introduction , 2 Room Temperature Tryptophan Phosphorescence of Proteins of Isolated Human Erythrocyte Membranes3 Room Temperature Tryptophan Phosphorescence of Plant Lectins in Solution; 3.1 Concanavalin A (Con A); 3.2 Phytohemagglutinin-L (PHA-L); 3.3 Wheat Germ Agglutinin (WGA); 3.4 Peanut Agglutinin (PNA); 3.5 Pisum sativum Agglutinin (PSA); 3.6 Sambucus nigra Agglutinin (SNA-I); 3.7 Laburnum anagyroides Lectin (LAL); 3.8 Solanum tuberosum Agglutinin (STA); References; Rational Design of FRET-Based Sensor Proteins; 1 Introduction , 2 Factors That Affect the Ratiometric Change in FRET-Based Sensor Proteins3 Quantitative Understanding of Energy Transfer by Modeling the Conformational Behavior of Flexible Linkers; 4 Quantitative Understanding of the Effect of Flexible Peptide Linkers on Effective Concentration; 5 Chelating Fluorescent Protein Chimeras as Efficient Zn(II) Sensor Proteins; 6 Taking Advantage of Stickiness: FRET Sensor Proteins Based on Conformational Switching; 7 Conclusion and Outlook; References; Fluorescence Imaging of Calcium Loading and Mitochondrial Depolarization in Cancer Cells Exposed to Heat Stress , 1 Introduction2 Fluorescence Imaging of Mitochondrial Calcium Loading; 2.1 The Role of Ca 2+ Inside Cells; 2.1.1 Fluorescent Ca 2+ Indicators; 2.2 Materials and Methods; 3 Results; 3.1 Cell Viability; 3.2 Visual Evaluation of Fluorescent Ca 2+ Indicators; 3.3 Fluorescence Imaging of Mitochondrial Transmembrane Potential; 4 Discussion; 5 Conclusions; References; Energy Transfer in Silica Nanoparticles: An Essential Toolfor the Amplification of the Fluorescence Signal; 1 Introduction; 2 The Principles of Energy Transfer Processes; 3 The Power of Energy Transfer Processes , 4 The Synthesis of Luminescent Silica Nanoparticles5 Photophysical Properties of Luminescent Silica Nanoparticles; 6 Conclusions; References; Spectroscopic Characterization of PlasmaChemically Functionalized and Fluorophore-Labeled Polymer Surfaces; 1 Introduction; 2 Surface Modification; 2.1 Surface Functionalization of Polypropylene; 2.2 Determination of Surface Functionalities with XPS; 2.3 Fluorophore Labeling of PP Surfaces; 3 Characterization of PP Films with Commonly Used Surface-Sensitive Techniques , 4 Fluorescence-Based Characterization of Labeled PP Surfaces Pitfalls and Troubleshooting , Electronic reproduction; Available via World Wide Web

Additional Edition: ISBN 9781441908285

Language: English

DOI: 10.1007/978-1-4419-1260-2

URL: Volltext  (lizenzpflichtig)

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Format: XIV, 490 p. 169 illus., 91 illus. in color. , online resource.

Edition: 1st ed. 2010.

ISBN: 9781441912602

Series Statement: Reviews in Fluorescence, 2008

Content: Reviews in Fluorescence 2008, the fifth volume of the book serial from Springer, serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence spectroscopy. It summarizes the year's progress in fluorescence and its applications, with authoritative analytical reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. Reviews in Fluorescence offers an essential reference material for any lab working in the fluorescence field and related areas. All academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in the continuously emerging field of fluorescence will find it an invaluable resource. Key features: Comprehensive coverage of the year's hottest and emerging topics. Chapters authored by known leading figures in the fluorescence field. Accessible utility in a single volume reference. New volume publishes annually Each Reviews in Fluorescence volume will be citable (ISI) and indexed. Reviews in Fluorescence 2008 topics include: Imaging Protein Interactions in Living Cells using the Fluorescent Proteins. Engineering Green Fluorescent Proteins Using an Expanded Genetic Code. The use of Fluorescence Anisotropy to Study the Preferential Orientation of Fluorophores in Organized Bi-Dimensional Systems. Fluorescence Imaging of Calcium Loading and Mitochondrial Depolarization in Cancer Cells Exposed to Heat Stress. Energy Transfer in Silica Nanoparticles: An Essential Tool for the Amplification Fluorescence Signals. Genetically Encoded Fluorescent and Bioluminescent Probes for Illuminating Cellular Signaling Pathways. Fluorescent Protein FRET Applications. About the Editor: Dr Chris D. Geddes, Ph.D., Professor, has extensive experience in fluorescence spectroscopy, particularly in fluorescence sensing and metal-fluorophore interactions (Metal-Enhanced Fluorescence), publishing over 190 papers and 18 books. Dr Geddes is internationally known in fluorescence for his scholarly publications and for the development of fluorescence-based plasmonics. He is the editor-in-chief of the Journal of Fluorescence and founding editor of the Who's Who in Fluorescence and Annual Reviews in Fluorescence volumes. In addition, due to the labs pioneering efforts in the fields of metallic nanoparticle-fluorophore interactions, Dr Geddes launched the Springer Journal "Plasmonics" in 2005. Dr Geddes is Director of the Institute of Fluorescence at the University of Maryland Biotechnology Institute which focuses on the nano-bio-technological applications of fluorescence. Dr Geddes frequently chairs NIH study sections, is a frequent member of the NIBIB special emphasis sensing panels and is currently a permanent member of the NIH EBT study section. http://theinstituteoffluorescence.com/.

Note: Fluorescence Anisotropy to Study the Preferential Orientation of Fluorophores in Ordered Bi-Dimensional Systems: Rhodamine 6G/Laponite Layered Films -- Room Temperature Tryptophan Phosphorescence of Proteins in the Composition of Biological Membranes and Solutions -- Rational Design of FRET-Based Sensor Proteins -- Fluorescence Imaging of Calcium Loading and Mitochondrial Depolarization in Cancer Cells Exposed to Heat Stress -- Energy Transfer in Silica Nanoparticles: An Essential Tool for the Amplification of the Fluorescence Signal -- Spectroscopic Characterization of Plasma - Chemically Functionalized and Fluorophore-Labeled Polymer Surfaces -- Fluorescent Labeling and Its Effect on Hybridization of Oligodeoxyribonucleotides -- New Method for Determining Histamine Rate in Halieutic Products -- Spectroscopy of DNA-Actinomycin Complexes -- Fluorescence Spectroscopy in Optoelectronics, Photomedicine, and Investigation of Biomolecular Systems -- Multicolor Imaging with Fluorescent Proteins in Mice -- Genetically Encoded Fluorescent and Bioluminescent Probes for Illuminating Cellular Signaling Pathways -- Fluorescent Protein FRET Applications -- Imaging Protein Interactions in Living Cells Using the Fluorescent Proteins -- Engineering Green Fluorescent Proteins Using an Expanded Genetic Code -- Fluorescent Proteins in Transgenic Plants -- Peptide Foldamers: From Spectroscopic Studies to Applications -- Circularly Polarized Luminescence (CPL) of Proteins and Protein Complexes -- New Dual Fluorescent Dyes Based on Modified "Excited State with Extended Conjunction" Photophysical Model -- Erratum.

In: Springer Nature eBook

Additional Edition: Printed edition: ISBN 9781441908292

Additional Edition: Printed edition: ISBN 9781441908285

Additional Edition: Printed edition: ISBN 9781461425724

Language: English

DOI: 10.1007/978-1-4419-1260-2

URL: https://doi.org/10.1007/978-1-4419-1260-2

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Format: XIII, 489 Seiten , Ill., graph. Darst.

Edition: 1

ISBN: 978-1-4419-0828-5

Language: English