Format:
Online-Ressource (X, 957 p. 132 illus., 79 illus. in color, digital)
Edition:
2nd ed. 2013
ISBN:
9781461439707
Series Statement:
SpringerLink
Content:
In the United States, hospitals annually report over 5 million cases of infectious-disease-related illnesses: clinical microbiology laboratories in these hospitals are engaged in detecting and identifying the pathogenic microorganisms in clinical specimens collected from these patients with suspected infections. Clearly, the timely and accurate detection/identification of these microbial pathogens is critical for patient treatment decisions and outcomes for millions of patients each year. Despite an appreciation that the outcome of an infectious-disease-related illness is directly related to the time required to detect and identify a microbial pathogen, clinical microbiology laboratories in the United States as well as worldwide have long been hampered by traditional culture-based assays, which may require prolonged incubation time for slowly growing microorganisms such as Mycobacterium tuberculosis. Moreover, traditional culture-based assays often require multiple steps with additional time needed for discernment of species and/or detection of antimicrobial resistance. Finally, these traditional, slow multistep culture-based assays are labor-intensive and required skilled clinical microbiologists at the bench.Over the past several decades, advanced molecular techniques in diagnostic microbiology quietly have been revolutionizing the practice of clinical microbiology in the hospital setting. Indeed, molecular diagnostic testing in general and nucleic-acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. There is no question that the development of rapid molecular techniques for nucleic acid amplification/characterization combined with automation and user-friendly software has greatly broadened the diagnostic capabilities of the clinical microbiology laboratory. These technical advances in molecular microbiology over the first decade of the 21st Century have profoundly influenced the physical structure of clinical microbiology laboratories as well as their staffing patterns, workflow, and turnaround time. These molecular microbiology advances have also resulted in the need for a revised and updated second edition of Advanced Techniques in Diagnostic Microbiology. This second edition again provides an updated and comprehensive description of the ongoing evolution of molecular methods for the diagnosis of infectious diseases. In addition, many new chapters have been added, including a chapter on the clinical interpretation and relevance of advanced technique results. The second edition, like the first edition, includes both a "techniques section describing the latest molecular techniques and an "applications section describing how these advanced molecular techniques are being used in the clinical setting. Finally, the second edition, like the first edition, utilizes a diverse team of authors who have compiled chapters that provide the reader with comprehensive and useable information on advanced molecular microbiology techniques.
Note:
Description based upon print version of record
,
Advanced Techniquesin Diagnostic Microbiology; Preface; Contents; Contributors; Part I: Methods; Chapter 1: Automated Blood Cultures; Introduction; Principles; Automated Culturing Systems; Blood Culture and CMBCS for Mycobacteria; Concluding Remarks; References; Chapter 2: Breath Tests for Detection of Helicobacter pylori and Aspergillus fumigatus; Introduction; Helicobacter pylori; Aspergillus spp.; Conventional Diagnostic Tests; Detection of H. pylori; Detection of Aspergillus spp.; Breath Tests; Introduction; Sample Collection; Urea Breath Test for H. pylori; Breath Test for Aspergillosis
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ConclusionsReferences; Chapter 3: Rapid Antigen Tests; Introduction; Principles of the Techniques; Agglutination; Immuno fl uorescence; Enzyme Immunoassay; Chemiluminescent Methods; Lateral Flow Immunochromatography and Fluoroimmunoassay; Characteristics of the Techniques; Applications of the Techniques; Bacteria; Fungi; Parasites; Viruses; References; Chapter 4: Antibody Detection: Principles and Applications; Introduction; Principles and Characteristics of Techniques; Colorimetric or Chromogenic Substrate; Radioimmunoassay; Chemiluminescence-Immunoassay; Electrochemiluminescent
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Single Enzyme Rapid TestsCatalase Test; Oxidase Test; Spot Indole Test; Slide Coagulase Test; Microdase; Bile Solubility Test; PYR; Leucine Aminopeptidase Test; MUG Test; Indoxyl Butyrate Disk; Chromogenic Enzyme Substrate Test; Gonocheck II; Bacticard Neisseria; Hippurate; Lysostaphin; CLO Test; Overnight Biochemical Tests; Tube Coagulase Test; DNA Hydrolysis; Vancomycin Disk Test; Bacitracin Inhibition Test (Taxo A Disk); Bacitracin and STX Susceptibility Test; Taxo P Disks (Optochin); CAMP Test; Reverse CAMP Test; Bile Esculin Agar Slant; 6.5 % Salt Broth; Indole Test; Nitrite Test
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ALA ( Haemophilus in fl uenzae Porphyrin Test)Motility Indole Lysine Sul fi de (MILS) Medium; O -Nitrophenyl-b- d -Galactopyranoside Test; Methyl Red Test; Voges-Proskauer Test; Pseudosel Agar Slant; Urea Agar Slant; Citrate Agar Slant; Cetrimide Agar; Gelatin; Acetate Utilization; Lead Acetate for Hydrogen Sul fi de Detection; Lysine Iron Agar; Triple Sugar Iron Agar Slant; Phenylalanine Deaminase; Decarboxylase (Moeller's Method); OF Glucose Medium; OF Sugars; Commercial Microbial Identi fi cation System; API Identi fi cation System; API Gram-Negative Identi fi cation
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API Gram-Positive Identi fi cation
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Fluorescent ImmunoassaysMulti-Analyte Pro fi le Technology; Contrast of These Techniques; Application of the Techniques in the Diagnostic Microbiology; Clinical Applications; Automation; Summary; References; Chapter 5: Cytometry-Based Antimicrobial Resistance Techniques; Introduction; Flow Cytometry Setup; Antimicrobial Resistance Measurements; Assay Turnaround Time; Comparator Methods; Flow Cytometry Challenges; Conclusions and Future Prospects; References; Chapter 6: Biochemical Pro fi le-Based Microbial Identi fi cation Systems; Introduction; Conventional Microbial Identi fi cation Systems
Additional Edition:
ISBN 9781461439691
Additional Edition:
Buchausg. u.d.T. Advanced techniques in diagnostic microbiology New York, NY [u.a.] : Springer, 2013 ISBN 9781461439691
Language:
English
Subjects:
Medicine
Keywords:
Mikrobiologische Diagnostik
DOI:
10.1007/978-1-4614-3970-7
URL:
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