UID:
kobvindex_HPB870994918
Format:
1 online resource (x, 123 pages) :
,
illustrations (some color)
ISBN:
9781780401775
,
1780401779
,
9781843392316
,
1843392313
Content:
The FISH Handbook for Biological Wastewater Treatment provides all the required information for the user to be able to identify and quantify important microorganisms in activated sludge and biofilms by using fluorescence in situ hybridization (FISH) and epifluorescence microscopy.
Note:
Cover -- Copyright -- Table of contents -- List of contributors -- Abbreviations -- 1. Introduction -- 1.1 Identification of microorganisms in activated sludge and biofilms -- 1.2 The microbiology of biological wastewater treatment -- 1.3 Factors of importance for the growth of microorganisms -- 1.4 The use of this FISH handbook -- 2. The Nitrifying Microbes: Ammonia Oxidizers, Nitrite Oxidizers, and Anaerobic Ammonium Oxidizers -- 2.1 Introduction -- 2.2 Ammonia oxidizers -- 2.2.1 Probes for the detection of AOB -- 2.3 Nitrite oxidizers -- 2.3.1 Probes for the detection of NOB -- 2.4 Anammox bacteria -- 2.4.1 Probes for the detection of anammox organisms -- 3. Identification of Denitrifying Microorganisms in Activated Sludge by Fish -- 3.1 Introduction -- 3.2 Identity of denitrifiers in wastewater treatment systems -- 3.3 Abundant denitrifiers in full-scale plants -- 3.4 Probes for detection of denitrifiers -- 4. Identification of Polyphosphate-Accumulating and Glycogen-Accumulating Organisms by Fish -- 4.1 Introduction -- 4.2 Identity of PAOs -- 4.2.1 Probes for detection of PAOs -- 4.3 Identity of GAOs -- 4.3.1 Probes for detection of GAOs -- 5. Identification of Filamentous Bacteria by Fish -- 5.1 Introduction -- 5.2 FISH detection of filamentous bacteria -- 5.3 Filamentous bacteria detection and identification -- 5.3.1 Beggiatoa morphotype -- 5.3.2 Haliscomenobacter hydrossis morphotype -- 5.3.3 Leucothrix mucor morphotype -- 5.3.4 Microthrix parvicella morphotype -- 5.3.5 Nostocoida limicola morphotypes -- 5.3.6 Nocardioform actinomycetes/Mycolata morphotype -- 5.3.7 Sphaerotilus natans and Leptothrix discophora morphotype -- 5.3.8 Streptococcus morphotype -- 5.3.9 Thiothrix and Type 021N morphotypes -- 5.3.10 0041/0675 morphotype -- 5.3.11 0092 morphotype -- 5.3.12 1701 morphotype -- 5.3.13 1851 morphotype -- 5.3.14 1863 morphotype.
,
5.3.15 0803, 0914 morphotypes and other still unidentified filamentous species -- 6. Identification of other Microorganisms in Activated Sludge and Biofilms by Fish -- 6.1 Introduction -- 6.2 Epiphytic bacteria involved in protein hydrolysis -- 6.3 Sulfate-reducing bacteria -- 6.4 Fermenting bacteria -- 6.5 Escherichia coli as indicator organism for entero-pathogens -- 7. Protocol for Fluorescence in Situ Hybridization (FISH) with rRNA-Targeted Oligonucleotides -- 7.1 Introduction -- 7.2 FISH protocol -- 7.2.1 Materials and solutions -- 7.2.2 Equipment and supplies needed for FISH -- 7.3 Protocol -- 7.3.1 Sample collection and fixation -- 7.3.2 Sample preparation -- 7.3.3 Immobilization of the samples on glass slides -- 7.3.4 Dehydration -- 7.3.5 Permeabilization by enzymatic or chemical treatment -- 7.3.6 Preparation and quality check of probes -- 7.3.7 Hybridization -- 7.3.8 Counterstaining with DAPI -- 7.4 Microscopy -- 7.5 Recommendations and troubleshooting -- 8. Quantitative FISH for the Cultivation-Independent Quantification of Microbes in Wastewater Treatment Plants -- 8.1 Introduction -- 8.2 Quantitative FISH: A brief overview -- 8.3 A protocol for quantitative FISH and image analysis to measure biovolume fractions -- 8.4 Concluding remarks -- 9. Color Image Section -- 10. Reference List -- Index.
Additional Edition:
Print version: FISH handbook for biological wastewater treatment ISBN 9781843392316
Language:
English
URL:
https://doi.org/10.2166/9781780401775
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