Kooperativer Bibliotheksverbund

UID:

Format: 1 online resource (555 pages)

Edition: 1st ed. 2024.

ISBN: 9783031410093

Series Statement: Springer Theses, Recognizing Outstanding Ph.D. Research,

Content: This book reports on the ecological engineering of granular sludge processes for a high-rate removal of carbon, nitrogen, and phosphorus nutrients in compact wastewater treatment plants. It provides novel insights into microorganisms and metabolisms in wastewater microbiomes and the use of microbial ecology principles to manage wastewater treatment processes. It covers a very comprehensive and inter-disciplinary research of systems microbiology and environmental biotechnology. From the initial economic assessment of the aerobic granular sludge technology, concepts of microbiome science and engineering are developed to uncover and manage the microbial ecosystem of granular sludge. Mixed-culture biotechnological processes, multifactorial experimental designs, laser scanning microscopy, molecular microbial ecology and bioinformatics methods, numerical ecology workflows, and mathematical modelling are engaged to disentangle granulation phenomena, microbial selection, and nutrient conversions across scales. The findings are assembled in a guideline for microbial resource management in granular sludge processes to support knowledge utilization in engineering practice. Outputs are integrated in the state of the art of biological wastewater treatment. This book addresses both scientists and engineers who are eager to get insights into and engineer microbiomes for environmental biotechnologies. It makes a valuable contribution to methods for strengthening the role of wastewater treatment plants for recovering safe water and resources, in the context of circular economy and for sustaining health and the environment in an ecologically balanced society.

Note: General Introduction & Economic Analysis -- Granular Sludge – State of the Art -- Research Questions & Scientific Overview -- Microbial selection during granulation of activated sludge under wash-out dynamics -- Concluding Remarks & Outlook.

Additional Edition: ISBN 9783031410086

Language: English

DOI: 10.1007/978-3-031-41009-3

URL: Volltext  (URL des Erstveröffentlichers)

URL: lizenzpflichtig

UID:

Format: 1 online resource (555 pages)

Edition: First edition.

ISBN: 9783031410093

Series Statement: Springer Theses Series

Note: Intro -- Supervisor's Foreword -- Concepts developed in this thesis contributed to the following publications: -- Critical Review -- Original Research Articles -- Technical Articles -- Peer-Reviewed Conference Proceedings -- Keynote Presentations and Invited Talks -- Oral Presentations -- Poster Presentations -- Peer-Reviewed Conference Abstracts and Talks -- Oral Presentations -- Poster Presentations -- Acknowledgments -- Contents -- Symbols and Abbreviations -- Base -- Acronyms -- Lumped Variables -- Organisms -- Chemical Compounds (Used as Abbreviations or Indices) -- Mathematical Symbols and Abbreviations, with Units -- Greek Mathematical Symbols -- Processes -- Environmental Conditions -- Compartments -- Other Indices -- References -- 1 General Introduction and Economic Analysis -- 1.1 Introduction -- 1.2 New Challenges for Wastewater Treatment Industries -- 1.3 Peak Phosphorus and Biological Methods of Urban Mining -- 1.4 Transitioning to a More Sustainable Management of Wastewater -- 1.5 Aerobic Granular Sludge Technology -- 1.5.1 Advantages of Aerobic Granular Sludge for a High-Rate BNR -- 1.5.2 The Flexibility of the SBR Technology -- 1.5.3 Granular Sludge for BNR Process Intensification -- 1.6 Economic Assessment of the Aerobic Granular Sludge Technology for a Swiss WWTP Operated for Full BNR -- 1.6.1 Reference WWTP -- 1.6.2 Assumptions -- 1.6.3 Economic Analysis -- 1.7 Conclusion -- References -- 2 Granular Sludge-State of the Art -- 2.1 Introduction -- 2.2 Biological Nutrient Removal from Wastewater -- 2.2.1 Microorganisms for Biological Nutrient Removal -- 2.2.2 The Cycling Metabolism of Polyphosphate-Accumulating Organisms -- 2.3 Biofilms -- 2.3.1 Interactions at Interfaces -- 2.3.2 Functional Sophistication of Biofilms: The Role of EPS -- 2.3.3 Mass Transfer Limited Ecosystems. , 2.3.4 Ecologically and Metabolically Diverse Habitats -- 2.4 High-Rate Biofilm Process Engineering -- 2.4.1 Biofilm Process Configurations -- 2.4.2 Features of Biofilm Reactor Systems -- 2.5 Self-aggregation of Microorganisms -- 2.5.1 Microbial Aggregation and Flocculation -- 2.5.2 Microorganisms and Adhesins in Floc Formation -- 2.5.3 Granular Methanogenic Sludge -- 2.6 Granular Sludge for a High-Rate Nutrient Removal -- 2.6.1 Initial Observations and Investigations of Aerobic Granular Sludge -- 2.6.2 Aerobic Granulation Mechanisms -- 2.6.3 Physical Factors of Granulation -- 2.6.4 Physical Characteristics of Granules for BNR -- 2.6.5 Multiphase Flow Dynamics in AGS Sequencing Batch Reactors -- 2.6.6 Importance of Extracellular Polymeric Substances in Granulation -- 2.6.7 Slow-Settling Filamentous Bulking Granules, and Remedial Actions -- 2.6.8 Full Biological Nutrient Removal in AGS-SBRs -- 2.6.9 Issues in the Start-Up of BNR Granular Sludge Systems After Seeding with Flocculent Activated Sludge -- 2.6.10 Design of Granular Sludge Reactors for BNR -- 2.6.11 Practical Implications for Implementing the Granular Sludge Technology for BNR at Full Scale -- 2.7 Microbial Ecology of Wastewater Treatment Systems -- 2.7.1 Microbial Communities and Climax -- 2.7.2 Microbial Ecology and Its Analytical Toolbox for Environmental Biotechnologies -- 2.7.3 The Saga of PAOs in EBPR Processes -- 2.7.4 A Holistic View on the BNR Microbiome -- 2.7.5 A Conceptual Model of the Microbial Ecosystem of BNR Processes -- 2.7.6 Selecting for Microorganisms with BNR Activities -- 2.7.7 Selecting for Polyphosphate-Accumulating Organisms Over Their Competitors -- 2.7.8 Resolving Molecular and Metabolic Signatures of PAOs and GAOs -- 2.8 Microbial Ecology of AGS Systems. , 2.8.1 Out-Selecting Filamentous Populations and Selecting Floc-Forming BNR Microorganisms in Granules by Managing Selective Pressures -- 2.8.2 Competition of PAOs and GAOs in Granular Sludge -- 2.8.3 Favoring Aerobic-Anoxic Gradients for PAOs, GAOs, Nitrifiers and Denitrifiers Inside BNR Granules -- 2.8.4 Toward an Ecological Engineering of Granular Sludge Using Principles of Microbial Ecology -- 2.9 Mathematical Modelling of Activated Sludge, Biofilm, and Granular Sludge Systems -- 2.9.1 Mathematical Modelling of Activated Sludge Systems -- 2.9.2 Modelling Biofilm Systems Across Length and Time Scales -- 2.9.3 Mathematical Modelling of BNR Granular Sludge Systems -- 2.10 Situation Analysis of the Wastewater Engineering and Molecular Biology Research -- 2.11 Conclusion -- References -- 3 Research Questions and Scientific Overview -- 3.1 Motivation and Scope of This Scientific Research -- 3.2 Research Questions and Scientific Overview -- References -- 4 Infrastructure and Flexible Bioreactor Design for Experimental Research with Granular Sludge -- 4.1 Introduction -- 4.2 Material and Methods -- 4.2.1 Bubble-Column Reactor Designs -- 4.2.2 Stirred-Tank Reactor Design -- 4.2.3 Implementation of Sequencing Batch Reactor Operations -- 4.2.4 On-Line Sensors and Amplifiers -- 4.2.5 Influent De-oxygenation Unit -- 4.3 Results and Discussion -- 4.3.1 Practicability of Bubble-Column SBR Designs -- 4.3.2 Troubleshooting During Operation of New Bioprocess SBR Infrastructures -- 4.3.3 Efficiency of the Influent De-oxygenation Unit -- 4.3.4 The Use of an Anaerobic Buffer Tank in Practice -- 4.4 Conclusions -- Supplementary Information -- References -- 5 PyroTRF-ID: A Bioinformatics Methodology for Profiling Microbiomes with T-RLFP and Amplicon Sequencing Data -- 5.1 Introduction -- 5.2 Material and Methods -- 5.2.1 Biological Samples -- 5.2.2 DNA Extraction. , 5.2.3 Experimental T-RFLP -- 5.2.4 Cloning and Sequencing -- 5.2.5 High-Throughput Amplicon Sequencing -- 5.2.6 Development of the PyroTRF-ID Bioinformatics Methodology -- 5.2.7 Optimization and Testing of PyroTRF-ID -- 5.3 Results -- 5.3.1 Pyrosequencing Quality Control and Read Length Limitation -- 5.3.2 Effect of Denoising and Mapping Procedures -- 5.3.3 Generation of Digital T-RFLP Profiles -- 5.3.4 Comparison of Digital and Experimental T-RFLP Profiles -- 5.3.5 Impact of Sequence Processing Steps, Pyrosequencing Methods and Sample Types -- 5.3.6 Efficiency of Phylogenetic Affiliation of T-RFs -- 5.4 Discussion -- 5.4.1 Advantages and Novelties of the PyroTRF-ID Bioinformatics Methodology -- 5.4.2 Performance Assessment and Limitations of PyroTRF-ID -- 5.4.3 Comparison of Community Compositions Obtained with PyroTRF-ID and MG-RAST -- 5.5 Conclusions -- Appendix: Phylogenetic Affiliations Obtained with PyroTRF-ID -- Description of Biological Samples Processed -- Predominant Bacterial Phylotypes and Corresponding OTUs -- Predominant OTUs and Corresponding Bacterial Phylotypes -- All Bacterial Phylotypes and Their Corresponding OTUs -- All OTUs and Their Corresponding Bacterial Phylotypes -- Supplementary Information -- References -- 6 Multilevel Correlations in the Metabolism of Polyphosphate-Accumulating Organisms -- 6.1 Introduction -- 6.2 Material and Methods -- 6.2.1 Cultivation of PAO- and GAO-Enrichments -- 6.2.2 Bacterial Community Compositions of PAO- and GAO-Enrichments -- 6.2.3 Conductivity-Based Anaerobic Metabolic Batch Tests -- 6.2.4 Implementation of a PAO/GAO Metabolic Model in PHREEQC -- 6.2.5 Polyphosphatase Enzymatic Assay -- 6.2.6 Degenerate PCR for the Screening of PPX Genes -- 6.3 Results -- 6.3.1 Principal Component Analysis of PAO- and GAO-Enrichment Conditions. , 6.3.2 Typical Profiles of Soluble Compounds Recorded in the PAO-SBR -- 6.3.3 Obtaining a Stable PAO-Enrichment by Control of OLR and Anaerobic Phase Length -- 6.3.4 Continuous Bacterial Community Monitoring of PAO- and GAO-Enrichments -- 6.3.5 Composition of the Bacterial Microbiomes of the PAO- and GAO-Enrichments -- 6.3.6 Correlation Between Conductivity Profiles and PAO/GAO Ratios -- 6.3.7 Model-Based Evaluation of Conductivity Evolutions in Anaerobic Metabolic Batch Tests -- 6.3.8 Correlating Polyphosphatase Activity and PAO/GAO Ratios -- 6.3.9 Screening PPX Genes in Activated Sludge and PAO-Enrichment -- 6.4 Discussion -- 6.4.1 Environmental Triggers for PAOs and GAOs Selection -- 6.4.2 The Quest for Stable Enrichment Cultures and EBPR Processes -- 6.4.3 Fast Assessment of PAO Fractions and EBPR Potential of Sludge -- 6.4.4 The Quest for PPX Genes in Activated Sludge -- 6.5 Conclusions -- Appendix -- Supplementary Information -- References -- 7 Microbial Selection During Granulation of Activated Sludge Under Wash-Out Dynamics -- 7.1 Introduction -- 7.2 Material and Methods -- 7.2.1 Reactor Infrastructure and Sequencing Batch Operation -- 7.2.2 Granulation Experiments -- 7.2.3 Characterizing Metabolic Activities of Inoculation Sludge Taken from the BNR-WWTP -- 7.2.4 Analyses of Soluble Compounds and Biomass -- 7.2.5 Molecular Analyses of Bacterial Community Compositions -- 7.2.6 Analysis of the Richness and Diversity of the Bacterial Community Evolving in Reactor R6 -- 7.2.7 Phylogenetic Affiliation of Operational Taxonomic Units -- 7.2.8 Bacterial Microbiome Analysis -- 7.3 Results -- 7.3.1 Composition and Activity of Early-Stage Granules Cultivated from OMR-Sludge -- 7.3.2 Composition and Activities of Early-Stage Granules Cultivated from BNR-Sludge -- 7.3.3 Dynamics of Process Performance and Bacterial Populations Under Wash-Out Conditions. , 7.3.4 Analysis of the Bacterial Microbiome of the Flocculent and Granular Sludges in R6.

Additional Edition: ISBN 9783031410086

Language: English

UID:

Format: XXXVI, 525 p. 89 illus., 65 illus. in color. , online resource.

Edition: 1st ed. 2024.

ISBN: 9783031410093

Series Statement: Springer Theses, Recognizing Outstanding Ph.D. Research,

Content: This book reports on the ecological engineering of granular sludge processes for a high-rate removal of carbon, nitrogen, and phosphorus nutrients in compact wastewater treatment plants. It provides novel insights into microorganisms and metabolisms in wastewater microbiomes and the use of microbial ecology principles to manage wastewater treatment processes. It covers a very comprehensive and inter-disciplinary research of systems microbiology and environmental biotechnology. From the initial economic assessment of the aerobic granular sludge technology, concepts of microbiome science and engineering are developed to uncover and manage the microbial ecosystem of granular sludge. Mixed-culture biotechnological processes, multifactorial experimental designs, laser scanning microscopy, molecular microbial ecology and bioinformatics methods, numerical ecology workflows, and mathematical modelling are engaged to disentangle granulation phenomena, microbial selection, and nutrient conversions across scales. The findings are assembled in a guideline for microbial resource management in granular sludge processes to support knowledge utilization in engineering practice. Outputs are integrated in the state of the art of biological wastewater treatment. This book addresses both scientists and engineers who are eager to get insights into and engineer microbiomes for environmental biotechnologies. It makes a valuable contribution to methods for strengthening the role of wastewater treatment plants for recovering safe water and resources, in the context of circular economy and for sustaining health and the environment in an ecologically balanced society.

Note: General Introduction & Economic Analysis -- Granular Sludge - State of the Art -- Research Questions & Scientific Overview -- Microbial selection during granulation of activated sludge under wash-out dynamics -- Concluding Remarks & Outlook.

In: Springer Nature eBook

Additional Edition: Printed edition: ISBN 9783031410086

Additional Edition: Printed edition: ISBN 9783031410109

Additional Edition: Printed edition: ISBN 9783031410116

Language: English

DOI: 10.1007/978-3-031-41009-3

URL: https://doi.org/10.1007/978-3-031-41009-3