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UID:

Umfang: 1 Online-Ressource (VII, 174 S.) , Ill.

ISBN: 9783110411324 , 9783110411331 , 9783110426250

Sprache: Englisch

Fachgebiete: Biologie

Schlagwort(e): Biochemie ; Labortechnik

DOI: 10.2478/9783110411331

URL: Volltext  (kostenfrei)

URL: Volltext  (kostenfrei)

UID:

Umfang: 1 online resource (186 pages) : , illustrations, (some colour)

Ausgabe: 1st ed.

ISBN: 1-5231-0070-2 , 3-11-042625-0 , 3-11-041133-4

Inhalt: Biochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.

Anmerkung: Bibliographic Level Mode of Issuance: Monograph , Front matter -- , Preface -- , Contents -- , 1 Introducing the Bacterial Antibiotic Sensor Mini Project -- , 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- , 3 Designing Primers for Site-Directed Mutagenesis -- , 4 Performing Site-Directed Mutagenesis -- , 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- , 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- , 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- , 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- , 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- , 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- , 11 Understanding the Importance of Buffers in Biological Systems -- , 12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- , 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- , 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- , List of Figures , Also available in print form. , English

Weitere Ausg.: ISBN 3-11-041132-6

Sprache: Englisch

DOI: 10.2478/9783110411331

UID:

Umfang: 1 Online-Ressource (186p.))

ISBN: 9783110411331

Inhalt: Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic

Weitere Ausg.: ISBN 9783110411324

Weitere Ausg.: Druck-Ausgabe ISBN 9783110411324

Sprache: Englisch

Fachgebiete: Biologie

DOI: 10.2478/9783110411331

URL: Volltext  (Open Access)

URL: Open Access

URL: Cover

URL: Cover

URL: Volltext  (lizenzpflichtig)

UID:

Umfang: Online-Ressource (VII, 174 S.) , Ill.

Ausgabe: 2015

ISBN: 9783110426250 , 9783110411324 , 9783110411331

Inhalt: Main description: Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.

Inhalt: Biographical note: Timea Gerczei Fernandez, Ball State University, Muncie, USAScott Patison, Ball State University, Muncie, USA

Anmerkung: FrontmatterPrefaceContents1 Introducing the Bacterial Antibiotic Sensor Mini Project2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function3 Designing Primers for Site-Directed Mutagenesis4 Performing Site-Directed Mutagenesis5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy11 Understanding the Importance of Buffers in Biological Systems12 Molecular Visualization of an Enzyme, Acetylcholinesterase13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment14 Separation of the Phosphatidylcholines Using Reverse Phase HPLCList of Figures.

Sprache: Englisch

Schlagwort(e): Biochemie ; Labortechnik

DOI: 10.2478/9783110411331

URL: Volltext  (kostenfrei)

URL: Cover

UID:

Umfang: 1 online resource (186 pages) : , illustrations, (some color)

ISBN: 9783110411331 (e-book)

Weitere Ausg.: Print version: Fernandez, Timea. Biochemistry laboratory manual for undergraduates : an inquiry-based approach. Warsaw, [Poland] ; Berlin, [Germany] : De Gruyter Open, c2014 ISBN 9783110411324

Sprache: Englisch

Schlagwort(e): Electronic books.

URL: Click to View

UID:

Umfang: 1 online resource (186 pages) : , illustrations (some color)

ISBN: 9783110411331 , 3110411334 , 9781523100705 , 1523100702 , 3110411326 , 9783110411324

Inhalt: Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.

Anmerkung: Machine generated contents note: 1. Introducing the Bacterial Antibiotic Sensor Mini Project -- 1.1. What are Antibiotics? -- 1.2. What is Bacterial Antibiotic Resistance? -- 1.3. How Do the Bacteria Detect Antibiotics In Its Environment? -- 1.4. How Does the ykkCD Sensor Exert Its Function? -- 1.5. What Do We Do During the Mini Project? -- 2. Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- 2.1. Learning Objectives -- 2.2. Mini Project Flowchart -- 2.3. Why is Sequence Conservation Important for Macromolecule Function, and How Do We Determine This? -- 2.4. Review of Nucleic Acid Properties -- 2.5. What is Bioinformatics? -- 2.6. Identifying Conserved Sequence Elements (Invariable Blocks) -- 2.7. Identifying Conserved Structural Elements -- BLAST Prelab -- Identifying Invariable Blocks in the Toxin Sensor Lab Report Outline and Point Distribution -- BLAst Problem Set -- Protein Properties Worksheet. , Note continued: 3. Designing Primers for Site-Directed Mutagenesis -- 3.1. Learning Objectives -- 3.2. Mini Project Flowchart -- 3.3. What is PCR? What are polymerases? -- 3.4. PCR Amplification of a Desired DNA Segment Of The Genome (Conventional Cloning) -- 3.5. Quickchange Site-Directed Mutagenesis -- Prelab Questions for Primer Design Lab -- Introduction to Primer Design Lab Report Outline and Point Distribution -- 4. Performing Site-Directed Mutagenesis -- 4.1. Learning Objective -- 4.2. Mini Project Flowchart -- 4.3. Review of Nucleic Acid Structure -- 4.4. How do Polymerases Work? -- 4.5. Polymerase Chain Reaction (PCR) in Practice -- 4.6. Why Did PCR Only Become Widely Available in the 1980s? -- 4.7. Applications of PCR -- Prelab Questions for Site-Directed Mutagenesis -- Site-directed Mutagenesis Lab Report Outline and Point Distribution -- PCR Worksheet. , Note continued: 5. Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- 5.1. Learning Objective -- 5.2. Mini Project Flowchart -- 5.3. Purification of Plasmid DNA from Bacterial Cell (Plasmid Prep) -- 5.4. Transformation -- 5.5. Cell Growth -- 5.6. Purification of Plasmid DNA from Bacterial Cells -- 5.7. Agarose Gel Electrophoresis -- 5.8. Application of Agarose Gel Electrophoresis -- 5.9. DNA Quality Control Using UV Spectroscopy -- Prelab Questions for Plasmid Prep -- DNA Purification Lab Report Outline and Point Distribution -- Electrophoresis Problem Set -- 6. Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- 6.1. Learning Objective -- 6.2. Mini Project Flowchart -- 6.3. Synopsis -- 6.4. How do Restriction Endonucleases Work? -- 6.5. How do Restriction Enzymes Achieve Million-Fold Specificity? , Note continued: 6.6. How Do We Judge Whether The Plasmid DNA is Successfully Linearized? -- 6.7. What are We Going to do in the Lab? -- Prelab Questions for DNA Linearization -- DNA Linearization Lab Report Outline and Point Distribution -- Worksheet -- Restriction Endonucleases -- Cloning Experiment Design -- Worksheet -- 7. Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- 7.1. Learning Objective -- 7.2. Mini Project Flowchart -- 7.3. How do RNA Polymerases Work? -- 7.4. How Does Transcription Start? -- 7.5. How Does Transcription End? -- 7.6. Transcription in Practice -- 7.7. What Are We Going To Do Today? -- Prelab Questions for RNA Transcription -- RNA Synthesis Lab Report Outline and Point Distribution -- 8. Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing Page and UV spectrometry -- 8.1. Learning Objective -- 8.2. Mini Project Flowchart -- 8.3. RNA Purification Methods -- 8.4. Denaturing Page -- 8.5. Phenol/chloroform Extraction. , Note continued: 8.6. Column Purification -- Prelab Questions for RNA Purification -- RNA Purification Lab Report Outline and Point Distribution -- 9. Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- 9.1. Learning Objective -- 9.2. Mini Project Flowchart -- 9.3. What is Binding Affinity (KD)? -- 9.4. What is Fluorescence? -- 9.5. How Do We Measure Binding Affinity of the Tetracycline-Sensor RNA Complex? -- 9.6. How do We Evaluate Binding Affinity? -- 9.7. How do We Analyze Data? -- Analysis of Binding Experiments -- Binding Assays Prelab -- YkkCD sensor RNA -- Tetracycline Binding Lab Report Outline and Point Distribution -- 10. Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- 10.1. Learning Objectives -- 10.2. Biological Role of Serum Albumin -- 10.3. Fluoroquinoline Antibiotics -- 10.4. Protein Structure, Aromatic Amino Acids, and Fluorescence. , Note continued: 10.5. Measuring Fluorescence -- 10.6. Synchronous Spectroscopy -- 10.7. Data Analysis -- Albumin -- Levofloxacin Binding Lab Report Outline and Point Distribution -- 11. Understanding the Importance of Buffers in Biological Systems -- 11.1. Learning Objectives -- 11.2. Introduction -- 11.3. Buffer Preparation -- Prelab for the Buffer Lab -- Buffer Lab Report Outline and Point Distributions -- Buffer Problem Set -- 12. Molecular Visualization of an Enzyme, Acetylcholinesterase -- 12.1. Learning Objectives -- 12.2. Introduction and Background -- 12.3. Introduction to Molecular Visualization Using the Program Chimera -- 12.4. Analysis of Acethylcholinesterase Using the Computer Visualization Program Chimera -- Molecular Visualization of Acethylcholinesterase Prelab -- Acetylcholinesterase Characteristics Worksheet -- 13. Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- 13.1. Learning Objective. , Note continued: 13.2. Measuring the Catalytic Efficiency of Acetylcholinesterase -- 13.3. Running a Steady-State Enzyme Kinetics Experiment -- 13.4. Designing a Steady-State Experiment -- Prelab for AchE Kinetics -- Lab Report Outline and Point Distribution -- Enzyme Kinetics Worksheet -- 14. Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- 14.1. Learning Objective -- 14.2. Phosphatidylcholines -- 14.3. High Performance Liquid Chromatography (HPLC) -- 14.4. Quantifying Chromatography -- HPLC of Lipids Prelab -- HPLC of Phosphatidylcholines Lab Report Outline and Point Distribution -- HPLC Problem Set.

Weitere Ausg.: Print version: Fernandez, Timea. Biochemistry laboratory manual for undergraduates : an inquiry-based approach. Warsaw, [Poland] ; Berlin, [Germany] : De Gruyter Open, ©2014 9783110411324

Sprache: Englisch

Schlagwort(e): Electronic books. ; Electronic books. ; Laboratory manuals.

URL: De Gruyter

URL: De Gruyter

URL: De Gruyter

URL: http://bibpurl.oclc.org/web/94223

URL: De Gruyter

URL: ebrary

URL: Knovel

URL: ProQuest Ebook Central

UID:

Umfang: VII, 174 S. : , Ill., graph. Darst. ; , 240 mm x 170 mm, 656 g.

ISBN: 978-3-11-041132-4 , 3-11-041132-6

Sprache: Englisch

Fachgebiete: Biologie

Schlagwort(e): Biochemie ; Labortechnik

URL: Inhaltstext

UID:

Umfang: 1 online resource (186 pages) : , illustrations, (some colour)

Ausgabe: 1st ed.

ISBN: 1-5231-0070-2 , 3-11-042625-0 , 3-11-041133-4

Inhalt: Biochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.

Anmerkung: Bibliographic Level Mode of Issuance: Monograph , Front matter -- , Preface -- , Contents -- , 1 Introducing the Bacterial Antibiotic Sensor Mini Project -- , 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- , 3 Designing Primers for Site-Directed Mutagenesis -- , 4 Performing Site-Directed Mutagenesis -- , 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- , 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- , 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- , 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- , 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- , 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- , 11 Understanding the Importance of Buffers in Biological Systems -- , 12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- , 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- , 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- , List of Figures , Also available in print form. , English

Weitere Ausg.: ISBN 3-11-041132-6

Sprache: Englisch

DOI: 10.2478/9783110411331

UID:

Umfang: 1 online resource (186 pages) : , illustrations, (some colour)

Ausgabe: 1st ed.

ISBN: 1-5231-0070-2 , 3-11-042625-0 , 3-11-041133-4

Inhalt: Biochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.

Anmerkung: Bibliographic Level Mode of Issuance: Monograph , Front matter -- , Preface -- , Contents -- , 1 Introducing the Bacterial Antibiotic Sensor Mini Project -- , 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- , 3 Designing Primers for Site-Directed Mutagenesis -- , 4 Performing Site-Directed Mutagenesis -- , 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- , 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- , 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- , 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- , 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- , 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- , 11 Understanding the Importance of Buffers in Biological Systems -- , 12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- , 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- , 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- , List of Figures , Also available in print form. , English

Weitere Ausg.: ISBN 3-11-041132-6

Sprache: Englisch

DOI: 10.2478/9783110411331