UID:
almahu_9947366356502882
Format:
1 online resource (614 p.)
ISBN:
1-281-01099-5
,
9786611010997
,
0-08-049670-9
Series Statement:
Methods in enzymology ; v. 313
Content:
Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedical-oriented research.The critically acclaime
Note:
Description based upon print version of record.
,
Front Cover; Antisense Technology; Copyright Page; Table of Contents; Contributors to Volume 313; Preface; Volumes In Series; Section I: General Methods; Chapter 1. Progress in Antisense Technology: The End of the Beginning; Chapter 2. Basic Principles of Using Antisense Oligonucleotides in Vivo; Chapter 3. Polyethyleneimine-Mediated Transfection to Improve Antisense Activity of 3' -Capped Phosphodiester Oligonucleotides; Chapter 4. Design of Antisense and Triplex-Forming Oligonucleotides
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Chapter 5. Chimeric Oligodeoxynucleotide Analogs: Chemical Synthesis, Purification, and Molecular and Cellular Biology ProtocolsChapter 6. Evaluation of Antisense Mechanisms of Action; Chapter 7. Physiology and Molecular Biology Brought to Single-Cell Level; Chapter 8. Antisense Properties of Peptide Nucleic Acid; Chapter 9. Synthesis of Oligonucleotide Conjugates in Anhydrous Dimethyl Sulfoxide; Chapter 10. Gene Switching: Analyzing Broad Range of Mutations Using Steric Block Antisense Oligonucleotides
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Chapter 11. Vesicular Stomatitis Virus as Model System for Studies of Antisense Oligonucleotide Translation ArrestChapter 12. Purification of Antisense Oligonucleotides; Chapter 13. Boranophosphate Backbone: A Mimic of Phosphodiesters, Phosphorothioates, and Methyl Phosphonates; Chapter 14. Intracellular Distribution of Digoxigenin-Labeled Phosphorothiate Oligonucleotides; Chapter 15. Use of Minimally Modified Antisense Oligonucleotides for Specific Inhibition of Gene Expression; Section II: Methods of Delivery
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Chapter 16. Determination of Cellular Internalization of Fluorescent OligonucleotidesChapter 17. Intrabody Tissue-Specific Delivery of Antisense Conjugates in Animals: Ligand-Linker Antisense Oligomer Conjugates; Chapter 18. Lipid-Based Formulations of Antisense Oligonucleotides for Systemic Delivery Applications; Chapter 19. In Vitro Transport and Delivery of Antisense Oligonucleotides; Chapter 20. In Vitro and in Vivo Delivery of Antisense Oligodeoxynucleotides Using Lipofection: Application of Antisense Technique to Growth Suppression of Experimental Glioma
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Chapter 21. Preparation and Application of Liposome-Incorporated OligodeoxynucleotidesChapter 22. Cell-Specific Optimization of Phosphorothioate Antisense Oligodeoxynucleotide Delivery by Cationic Lipids; Section III: RNA Studies; Chapter 23. Intracellular Expression and Function of Antisense Catalytic RNAs; Chapter 24. Selective Degradation of Targeted mRNAs Using Partially Modified Oligonucleotides; Chapter 25. Reversible Depletion of Specific RNAs by Antisense Oligodeoxynucleotide-Targeted Degradation in Frog Oocytes
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Chapter 26. Inactivation of Gene Expression Using Ribonuclease P and External Guide Sequences
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English
Additional Edition:
ISBN 0-12-182214-1
Language:
English
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