UID:
almahu_9947366356702882
Format:
1 online resource (998 p.)
ISBN:
1-281-01101-0
,
9786611011017
,
0-08-049672-5
Series Statement:
Methods in enzymology ; v. 315
Content:
This volume, and its companion Volume 316, include newly developed methods to study vertebrate phototransduction and the visual cycle. Major topics covered include expression, isolation, and characterization of opsins; proteins that interact with rhodopsin; transducin and regulators of G-protein signaling; photoreceptor protein phosphatases, phosphodiesterase, and guanylylcyclase; cyclis nucleolide gated channels; Na+/Ca2+-K+ exchanges and ABCR transporter.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly r
Note:
Description based upon print version of record.
,
Front Cover; Vertebrate Phototransduction and the Visual Cycle; Copyright Page; Table of Contents; Contributors to Volume 315; Preface; Volumes in Series; Section I: Expression and Isolation of Opsins; Chapter 1. Heterologous Expression of Bovine Opsin in Pichia pastoris; Chapter 2. Baculovirus Expression System for Expression and Characterization of Functional Recombinant Visual Pigments; Chapter 3. Development of Stable Cell Lines Expressing High Levels of Point Mutants of Human Opsin for Biochemical and Biophysical Studies
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Chapter 4. Folding and Assembly of Rhodopsin from Expressed FragmentsChapter 5. Isolation of Isoelectric Species of Phosphorylated Rhodopsin; Chapter 6. Rhodopsin Trafficking in Photoreceptors Using Retinal Cell-Free System; Section II: Characterization of Opsins; Chapter 7. Preparation and Analysis of Two-Dimensional Crystals of Rhodopsin; Chapter 8. Domain Approach to Three-Dimensional Structure of Rhodopsin Using High Resolution Nuclear Magnetic Resonance; Chapter 9. Analysis of Functional Microdomains in Rhodopsin
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Chapter 10. Mapping Tertiary Contacts between Amino Acid Residues within RhodopsinChapter 11. Energetics of Rhodopsin Photobleaching: Photocalorimetric Studies of Energy Storage in Early and Late Intermediates; Chapter 12. Absorption Spectroscopy in Studies of Visual Pigments. Spectral and Kinetic Characterization of Intermediates; Chapter 13. Structural Determinants of Active State Conformation of Rhodopsin. Molecular Biophysics Approaches; Chapter 14. pKa of Protonated Schiff Base of Visual Pigments; Chapter 15. Assays for Detection of Constitutively Active Opsins
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Chapter 16. Synthetic Retinals: Convenient Probes of Rhodopsin and Visual Transduction ProcessChapter 17. Assays for Activation of Opsin by all-trans-Retinal; Chapter 18. Assays for Activation of Recombinant Expressed Opsin by all-trans-Retinal; Chapter 19. Electrical Approach to Study Rhodopsin Activation in Single Cells with Early Receptor Current Assay; Chapter 20. Analysis of Amino Acid Residues in Rhodopsin and Cone Visual Pigments That Determine Their Molecular Properties; Chapter 21. Phylogenetic Analysis and Experimental Approaches to Study Color Vision in Vertebrates
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Section III: Proteins That Interact with RhodopsinChapter 22. Light Scattering Methods to Monitor Interaction between Rhodopsin-Containing Membranes and Soluble Proteins; Chapter 23. Heterogeneity of Rhodopsin Intermediate State Interacting with Transducin; Chapter 24. Limited Proteolytic Digestion Studies of G Protein Receptor Interaction; Chapter 25. Monitoring Proton Uptake from Aqueous Phase during Rhodopsin Activation; Chapter 26. Use of Peptides-on-Plasmids Combinatorial Library to Identify High Affinity Peptides That Bind Rhodopsin
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Chapter 27. Purifiation of Rhodopsin Kinase by Recoverin Affinity Chromatography
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English
Additional Edition:
ISBN 0-12-182216-8
Language:
English
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