UID:
almahu_9949697921202882
Umfang:
1 online resource (581 p.)
ISBN:
1-281-01107-X
,
9786611011079
,
0-08-049678-4
Serie:
Methods in enzymology ; v. 323
Inhalt:
Volume 323 of Methods in Enzymology is dedicated to the energetics of biological macromolecules. Understanding the molecular mechanisms underlying a biological process requires detailed knowledge of the structural relationships within the system and an equally detailed understanding of the energetic driving forces that control the structural interactions. This volume presents modern thermodynamic techniques currently being utilized to study the energetic driving forces in biological systems. It will be a useful reference source and textbook for scientists and students whose goal
Anmerkung:
Description based upon print version of record.
,
Front Cover; Energetics of Biological Macromolecules; Copyright Page; Table of Contents; Contributors to Volume; Preface; Volumes in Series; Chapter 1. Quantitative Dissection of Transcriptional Control System: N-Dependent Antitermination Complex of Phage ? as Regulatory Paradigm; Chapter 2. Problems and Prospects in Microcalorimetry of Biological Macromolecules; Chapter 3. Probing Stability of Helical Transmembrane Proteins; Chapter 4. Energetics of Vinca Alkaloid Interactions with Tubulin
,
Chapter 5. Kinetics and Thermodynamics of Conformational Equilibria in Native Proteins by Hydrogen ExchangeChapter 6. Mathematical Modeling of Cooperative Interactions in Hemoglobin; Chapter 7. Deriving Complex Ligand-Binding Formulas; Chapter 8. Calculation of Entropy Changes in Biological Processes. Folding, Binding, and Oligomerization; Chapter 9. Evaluating Energetics of Erythropoietin Ligand Binding to Homodimerized Receptor Extracellular Domains; Chapter 10. Measurement of Protein Interaction Bioenergetics. Application to Structural Variants of Anti-sCD4 Antibody
,
Chapter 11. Analysis of Interaction of Regulatory Protein TyrR with DNAChapter 12. Proteolytic Footprinting Titrations for Estimating Ligand-Binding Constants and Detecting Pathways of Conformational Switching of Calmodulin; Chapter 13. Analysis of Reversibly Interacting Macromolecular Systems by Time Derivative Sedimentation Velocity; Chapter 14. Kinetic, Equilibrium, and Thermodynamic Analysis of Macromolecular Interactions with BIACORE; Chapter 15. Structure-Function Relationships in Two-Component Phospholipid Bilayers: Monte Carlo Simulation Approach Using a Two-State Model
,
Chapter 16. Parsing Free Energies of Drug-DNA InteractionsChapter 17. Direct Measurement of Sodium Ion Release from DNA on Binding of Cationic Ligands; Chapter 18. Enthalpy-Entropy Compensations in Nucleic Acids. Contribution of Electrostriction and Structural Hydration; Chapter 19. Time-Resolved Fluorescence Methods for Analysis of DNA-Protein Interactions; Chapter 20. Use of Fluorescence Spectroscopy as Thermodynamics Tool; Chapter 21. Microsecond Dynamics of Biological Macromolecules; Author Index; Subject Index; Chapter 22. Errata
,
English
Weitere Ausg.:
ISBN 0-12-182224-9
Sprache:
Englisch
Bookmarklink
