UID:
almahu_9947366357602882
Umfang:
1 online resource (586 p.)
ISBN:
1-281-01108-8
,
9786611011086
,
0-08-049679-2
Serie:
Methods in enzymology ; v. 324
Inhalt:
Volume 324 of Methods in Enzymology supplements Volume 166. It includes genetic information (cloning, gene expression) and information on human genetic diseases not available when Volume 166 was published.General Description of the Series:The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than
Anmerkung:
Description based upon print version of record.
,
Front Cover; Branched-Chain Amino Acids; Copyright Page; Table of Contents; Contributors to Volume 324; Preface; Volumes in Series; Section I: Preparation of Substrates, Assays of Intermediates and Enzymes, and Use of Enzyme Inhibitors; Chapter 1. Synthesis and Gas Chromatography/Mass Spectrometry Analysis of Stereoisomers of 2-Hydroxy- 3-methylpentanoic Acid; Chapter 2. Analysis of Intracellular Metabolites as Tool for Studying Branched-Chain Amino Acid Biosynthesis and Its Inhibition in Bacteria; Chapter 3. Determination of Branched-Chain L-Amino-Acid Aminotransferase Activity
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Chapter 4. Analysis of (S)- and (R)-3-Methyl-2-oxopentanoate Enantiomorphs in Body FluidsChapter 5. Spectrophotometric Assay for Measuring Branched-Chain Amino Acids; Chapter 6. Determination of Branched-Chain a-Keto Acid Dehydrogenase Activity State and Branched-Chain a-Keto Acid Dehydrogenase Kinase Activity and Protein in Mammalian Tissues; Chapter 7. Simultaneous Quantification of Plasma Levels of a-Ketoisocaproate and Leucine by Gas Chromatography-Mass Spectrometry; Chapter 8. Synthesis of Methacrylyl-CoA and (R)- and (S)- Hydroxyisobutyryl-CoA
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Chapter 9. Pathways of Leucine and Valine Catabolism in YeastSection II: Cloning, Expression, and Purification of Enzymes of Branched-Chain Amino Acid Metabolism; Chapter 10. Isolation of Subunits of Acetohydroxy Acid Synthase Isozyme III and Reconstitution of Holoenzyme; Chapter 11. Branched-Chain Amino-Acid Aminotransferase of Escherichia coli; Chapter 12. Purification of Sodium-Coupled Branched-Chain Amino Acid Carrier of Pseudomonas aeruginosa; Chapter 13. Reconstitution of Pseudomonas aeruginosa High-Affinity Branched-Chain Amino Acid Transport System
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Chapter 14. Purification of Pseudomonas putida Branched-Chain Keto Acid Dehydrogenase E1 ComponentChapter 15. Pseudomonas mevalonii 3-Hydroxy-3-methylglutaryl-CoA Lyase; Chapter 16. Human 3-Hydroxy-3-methylglutaryl-CoA Lyase; Chapter 17. Branched-Chain a-Keto Acid Dehydrogenase Kinase; Chapter 18. Expression of E1 Component of Human Branched- Chain a-Keto Acid Dehydrogenase Complex in Escherichia coli by Cotransformation with Chaperonins GroEL and GroES
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Chapter 19. Production of Recombinant Mammalian Holo-E2 and E3 and Reconstitution of Functional Branched-Chain a-Keto Acid Dehydrogenase Complex with Recombinant E1Chapter 20. Production of Recombinant E1 Component of Branched-Chain a-Keto Acid Dehydrogenase Complex; Chapter 21. Mammalian Methylmalonate-Semialdehyde Dehydrogenase; Chapter 22. Mammalian 3-Hydroxyisobutyrate Dehydrogenase; Chapter 23. 3-Hydroxyisobutyryl-CoA Hydrolase; Chapter 24. Mammalian Branched-Chain Acyl-CoA Dehydrogenases: Molecular Cloning and Characterization of Recombinant Enzymes
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Chapter 25. 3-Hydroxy-3-methylglutaryl-CoA Reductase
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English
Weitere Ausg.:
ISBN 0-12-182225-7
Sprache:
Englisch
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