UID:
almahu_9949697923602882
Umfang:
1 online resource (592 p.)
ISBN:
1-281-01113-4
,
9786611011130
,
0-08-049684-9
Serie:
Methods in enzymology, v. 329
Inhalt:
Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport.This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.
Anmerkung:
Description based upon print version of record.
,
Front Cover; Regulators and Effectors of Small GTPases; Copyright Page; Table of Contents; Contributors to Volume 329; Preface; Volumes in Series; Section I: Rab GTPases; Chapter 1. Structural Basis for Rab Function: An Overview; Chapter 2. Expression of Wild-Type and Mutant Green Fluorescent Protein-Rab1 for Fluorescence Microscopy Analysis; Chapter 3. Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Escort Protein, and Geranylgeranyltransferase; Chapter 4. Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases
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Chapter 5. Antibody and Oligonucleotide Probes to Distin- guish Intracellular Expression and Localization Patterns of Rab GDP-Dissociation Inhibitor IsoformsChapter 6. Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Proteins of Gyp Family; Chapter 7. Purification and Properties of Rab3 GDP/GTP Exchange Protein; Chapter 8. Purification and Properties of Rab3 GTPase-Activating Protein; Chapter 9. Rabphilin-3: A Target Molecule for Rab3 Small G Proteins; Chapter 10. Doc2α as Modulator of Ca2+ -Dependent Exocytosis
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Chapter 11. Purification and Properties of Sec4p GTPase-Activating ProteinChapter 12. Purification and Characterization of Yeast Exocyst Complex; Chapter 13. Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling; Chapter 14. Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Activity Assays; Chapter 15 Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex-5; Chapter 16. Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis
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Chapter 17. Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their Interactions with Rab6AChapter 18. Expression and Functional Analyses of Rab8 and Rab11a in Exocytic Transport from trans-Golgi Network; Chapter 19. Expression and Properties of Rab7 in Endosome Function; Chapter 20. Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Organization and Polarized Transport; Chapter 21. Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase δ Subunit
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Chapter 22. Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial Cells Grown on Polycarbonate FiltersChapter 23. Expression and Properties of Rab25 in Polarized Madin-Darby Canine Kidney Cells; Chapter 24. Purification of TRAPPfrom Saccharomyces cerevisiae and Identification of Its Mammalian Counterpart; Section II: ADP-Ribosylation Factor (ARF) GTPases; Chapter 25. Structural and Functional Organization of ADP- Ribosylation Factor (ARF) Proteins; Chapter 26. Expression and Properties of ADP-Ribosylation Factor (ARF6) in Endocytic Pathway
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Chapter 27. Expression and Analysis of ARNO and ARNO Mutants and Their Effects on ADP-Ribosylation Factor (ARF)-Mediated Actin Cytoskeletal Rearrangements
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English
Weitere Ausg.:
ISBN 0-12-182230-3
Sprache:
Englisch
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