UID:
almahu_9947366361202882
Umfang:
1 online resource (667 p.)
ISBN:
1-281-01134-7
,
9786611011345
,
0-08-049705-5
Serie:
Methods in enzymology ; v.362
Inhalt:
Recognition of carbohydrates in biological systems has been gaining more and more attention in recent years. Although methodology for studying recognition has been developing, there is no volume that covers the wide area of methodology of carbohydrate recognition. This volume and its companion, Volume 363, present state-of-the-art methodologies, as well as the most recent biological observations in this area.* Covers the isolation/synthesis of substances used in studying interactions involving carbohydrates* Discussed the methodology for measuring such interactions* Biological
Anmerkung:
Description based upon print version of record.
,
Cover; Table of Contents; Contributors to Volume; Preface; Volumes in Series; Section I: Preparative Methods; Chapter 1. Transition Metal-Catalyzed Cross-Coupling Reactions toward the Synthesis of a-d- Mannopyranoside Clusters; Chapter 2. Ruthenium Carbenoids as Catalysts for Olefin Metathesis of w-Alkenyl Glycosides; Chapter 3. Digoxin Derivatives as Tools for Glycobiology; Chapter 4. Synthesis of Peptide-Based Trivalent Scaffold for Preparation of Cluster Glycosides; Chapter 5. Chemoenzymatic Synthesis of Diverse Asparagine-Linked Oligosaccharides
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Chapter 10. Conjugating Low Molecular Mass Carbohydrates to Proteins 1. Monitoring the Progress of ConjugationChapter 11. Conjugating Low Molecular Mass Carbohydrates to Proteins 2. Recovery of Excess Ligand Used in the Conjugation Reaction; Chapter 12. Neoglycolipid Technology: Deciphering Information Content of Glycome; Chapter 13. Preparation and Utility of Neoproteoglycan Probes in Analyses of Interaction with Glycosaminoglycan-Binding Proteins; Chapter 14. Modular Sunthesis and Study of Multivalent Carbohydrate Ligands with Long and Flexible Linkers
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Chapter 15. Sugar Arrays in Microtiter PlatesChapter 16. Comparative Studies of Galactoside- Containing Clusters; Chapter 17. Multivalent Breast Cancer T-Antigen Markers Scaffolded onto PAMAM Dendrimers; Chapter 18. Constructing Azide-Labeled Cell Surfaces Using Polysaccharide Biosynthetic Pathways; Chapter 19. Cell Wall Engineering of Living Bacteria through Biosynthesis; Chapter 20. Molecular Biological Fingerprinting of Human Lectin Expression by RT-PCR; Section II: General Techniques; Chapter 21. Visualization and Characterization of Receptor Clusters by Transmission Electron Microscopy
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Chapter 22. Exploring Kinetics and Mechanism of Protein- Sugar Recognition by Surface Plasmon ResonanceChapter 23. Quantitative Lectin-Carbohydrate Interaction Analysis on Solid-Phase Surfaces Using Biosensor Based on Surface Plasmon Resonance; Chapter 24. Surface Plasmon Resonance Study of RNA-Aminoglycoside Interactions; Chapter 25. Frontal Affinity Chromatography as a Tool for Elucidation of Sugar Recognition Properties of Lectins; Chapter 26. Evaluating Carbohydrate-Protein Binding Interactions Using Frontal Affinity Chromatography Coupled to Mass Spectrometry
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Chapter 27. Determination of Protein-Oligosaccharide Binding by Nanoelectrospray Fourier- Transform Ion Cyclotron Resonance Mass Spectrometry
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English
Weitere Ausg.:
ISBN 0-12-182265-6
Sprache:
Englisch
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