UID:
almahu_9947366365802882
Format:
1 online resource (842 p.)
ISBN:
1-281-05036-9
,
9786611050368
,
0-08-047939-1
Series Statement:
Methods in enzymology ; v. 406
Content:
The Ras superfamily (〉150 human members) encompasses Ras GTPases involved in cell proliferation, Rho GTPases involved in regulating the cytoskeleton, Rab GTPases involved in membrane targeting/fusion and a group of GTPases including Sar1, Arf, Arl and dynamin involved in vesicle budding/fission. These GTPases act as molecular switches and their activities are controlled by a large number of regulatory molecules that affect either GTP loading (guanine nucleotide exchange factors or GEFs) or GTP hydrolysis (GTPase activating proteins or GAPs). In their active state, they interact with a continua
Note:
Description based upon print version of record.
,
Cover; Copyright Page; Table of Contents; Contributors to Volume 406; Preface; Volumes in Series; Chapter 1: Purification and Biochemical Properties of Rac1, 2, 3 and the Splice Variant Rac1b; Chapter 2: Biochemical Analyses of the Wrch Atypical Rho Family GTPases; Chapter 3: Purification of P-Rex1 from Neutrophils and Nucleotide Exchange Assay; Chapter 4: In Vitro Guanine Nucleotide Exchange Activity of DHR-2/DOCKER/CZH2 Domains; Chapter 5: Biochemical Characterization of the Cool (Cloned-Out-of-Library)/Pix (Pak-Interactive Exchange Factor) Proteins
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Chapter 6: GEF and Glucosylation Assays on Liposome-Bound RacChapter 7: Phosphorylation of RhoGDI by p21-Activated Kinase 1; Chapter 8: Purification of ARAP3 and Characterization of GAP Activities; Chapter 9: Regulation of RhoGAP Specificity by Phospholipids and Prenylation; Chapter 10: Purification and Activity of the Rho ADP-Ribosylating Binary C2/C3 Toxin; Chapter 11: Purification of TAT-C3 Exoenzyme; Chapter 12: Imaging and Photobleach Correction of Mero-CBD, Sensor of Endogenous Cdc42 Activation; Chapter 13: Cdc42 and PI(4,5)P2-Induced Actin Assembly in Xenopus Egg Extracts
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Chapter 14: In Vitro Reconstitution of Cdc42-Mediated Actin Assembly Using Purified ComponentsChapter 15: Biochemical Analysis of Mammalian Formin Effects on Actin Dynamics; Chapter 16: Formin Proteins: Purification and Measurement of Effects on Actin Assembly; Chapter 17: Purification and Kinase Assay of PKN; Chapter 18: Purification and Enzyme Activity of ACK1; Chapter 19: Direct Activation of Purified Phospholipase C Epsilon by RhoA Studied in Reconstituted Phospholipid Vesicles; Chapter 20: Regulation of PLCbeta Isoforms by Rac
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Chapter 21: Biochemical Properties and Inhibitors of (N-)WASPChapter 22: The Use of GFP to Localize Rho GTPases in Living Cells; Chapter 23: Analysis of the Spatiotemporal Activation of Rho GTPases Using Raichu Probes; Chapter 24: Measurement of Activity of Rho GTPases During Mitosis; Chapter 25: Inhibition of Rho GTPases by RNA Interference; Chapter 26: RNA Interference Techniques to Study Epithelial.Cell Adhesion and Polarity; Chapter 27: Nucleofection of Primary Neurons; Chapter 28: Dock180-ELMO Cooperation in Rac Activation; Chapter 29: Rho GTPase Activation by Cell-Cell Adhesion
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Chapter 30: Activation of Rap1, Cdc42, and Rac by Nectin Adhesion SystemChapter 31: Analysis of Activated GAPs and GEFs in Cell Lysates; Chapter 32: Degradation of RhoA by Smurf1 Ubiquitin Ligase; Chapter 33: Ubiquitin-Mediated Proteasomal Degradation of Rho Proteins by the CNF1 Toxin; Chapter 34: Regulation of Superoxide-Producing NADPH Oxidases in Nonphagocytic Cells; Chapter 35: Activation of MEKK1 by Rho GTPases; Chapter 36: Activation of the Apoptotic JNK Pathway Through the Rac1-Binding Scaffold Protein POSH
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Chapter 37: Quantification of Isozyme-Specific Activation of Phospholipase C-beta2 by Rac GTPases and Phospholipase C-epsiv by Rho GTPases in an Intact Cell Assay System
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English
Additional Edition:
ISBN 0-12-182811-5
Language:
English
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