In:
FEBS Letters, Wiley, Vol. 591, No. 18 ( 2017-09), p. 2793-2802
Abstract:
Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP 55 and Rab2 in the medial‐Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl‐CoA binding domain containing 3 ( ACBD 3) is likely to be a novel binding partner of Golgin45. ACBD 3 interacts with Golgin45 via its GOLD domain, while its co‐expression significantly increases Golgin45 targeting to the Golgi. Furthermore, ACBD 3 recruits TBC 1D22, a Rab33b GTP ase activating protein ( GAP ), to a large multi‐protein complex containing Golgin45 and GRASP 55. These results suggest that ACBD 3 may provide a scaffolding to organize the Golgi stacking proteins and a Rab33b‐ GAP at the medial‐Golgi.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1002/feb2.2017.591.issue-18
DOI:
10.1002/1873-3468.12780
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
1460391-3
SSG:
12
