In:
FEBS Letters, Wiley, Vol. 592, No. 3 ( 2018-02), p. 318-331
Abstract:
Genome maintenance and integrity requires continuous alterations of the compaction state of the chromatin structure. Chromatin remodelers, among others the INO 80 complex, help organize chromatin by repositioning, reshaping, or evicting nucleosomes. We report on INO 80 nucleosome remodeling, assayed by single‐molecule Foerster resonance energy transfer on canonical nucleosomes as well as nucleosomes assembled from tailless histones. Nucleosome repositioning by INO 80 is a processively catalyzed reaction. During the initiation of remodeling, probed by the INO 80 bound state, the nucleosome reveals structurally heterogeneous states for tailless nucleosomes (in contrast to wild‐type nucleosomes). We, therefore, propose an altered energy landscape for the INO 80‐mediated nucleosome sliding reaction in the absence of histone tails.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1002/feb2.2018.592.issue-3
DOI:
10.1002/1873-3468.12973
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
1460391-3
SSG:
12