In:
FEBS Open Bio, Wiley, Vol. 9, No. 7 ( 2019-07), p. 1184-1193
Kurzfassung:
Voltage‐dependent anion channel isoform 2 of the yeast Saccharomyces cerevisiae ( yVDAC 2) was believed for many years to be devoid of channel activity. Recently, we isolated yVDAC 2 and showed that it exhibits channel‐forming activity in the planar lipid bilayer system when in its so‐called native form. Here, we describe an alternative strategy for yVDAC 2 isolation, through heterologous expression in bacteria and refolding in vitro . Recombinant yVDAC 2, like its native form, is able to form voltage‐dependent channels. However, some differences between native and recombinant yVDAC 2 emerged in terms of voltage dependence and ion selectivity, suggesting that, in this specific case, the recombinant protein might be depleted of post‐translational modification(s) that occur in eukaryotic cells.
Materialart:
Online-Ressource
ISSN:
2211-5463
,
2211-5463
DOI:
10.1002/feb4.2019.9.issue-7
DOI:
10.1002/2211-5463.12574
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2019
ZDB Id:
2651702-4