In:
Angewandte Chemie, Wiley, Vol. 128, No. 23 ( 2016-06), p. 6810-6813
Abstract:
In recent years, technologies capable of analyzing single cells have emerged that are transforming many fields of biological research. Herein we report how DNA‐functionalized hydrogel beads can serve as a matrix to capture mRNA from lysed single cells. mRNA quantification free of pre‐amplification bias is ensured by using padlock probes and rolling circle amplification followed by hybridization with fluorescent probes. The number of transcripts in individual cells is assessed by simply counting fluorescent dots inside gel beads. The method extends the potential of existing techniques and provides a general platform for capturing molecules of interest from single cells.
Type of Medium:
Online Resource
ISSN:
0044-8249
,
1521-3757
DOI:
10.1002/ange.v128.23
DOI:
10.1002/ange.201601969
Language:
English
Publisher:
Wiley
Publication Date:
2016
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505872-7
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1479266-7
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