In:
Angewandte Chemie International Edition, Wiley, Vol. 59, No. 43 ( 2020-10-19), p. 19202-19208
Abstract:
Distance‐dependent electromagnetic radiation and electron transfer have been commonly employed in washing‐free fluorescence and electrochemical bioassays, respectively. In this study, we combined the two distance‐dependent phenomena for sensitive washing‐free DNA detection. A distance‐dependent surface plasmonic field induces rapid photoredox catalysis of surface‐bound catalytic labels, and distance‐dependent mediated electron transfer allows for rapid electron transfer from the surface‐bound labels to the electrode. An optimal system consists of a chemically reversible acceptor (Ru(NH 3 ) 6 3+ ), a chemically reversible photoredox catalyst (eosin Y), and a chemically irreversible donor (triethanolamine). Side reactions with O 2 do not significantly decrease the efficiency of photoredox catalysis. Energy transfer quenching between the electrode and the label can be lowered by increasing the distance between them. Washing‐free DNA detection had a detection limit of approximately 0.3 n m in buffer and 0.4 n m in serum without a washing step.
Type of Medium:
Online Resource
ISSN:
1433-7851
,
1521-3773
DOI:
10.1002/anie.202007318
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
2011836-3
detail.hit.zdb_id:
123227-7